α -(2-羟基-2-甲基丙基)-ω-[2-羟基-3-甲基丁-2-烯-1-基]聚亚甲基抗皮肤真菌活性评价Flem。

K. Sagar, G. Vidyasagar
{"title":"α -(2-羟基-2-甲基丙基)-ω-[2-羟基-3-甲基丁-2-烯-1-基]聚亚甲基抗皮肤真菌活性评价Flem。","authors":"K. Sagar, G. Vidyasagar","doi":"10.5580/19cf","DOIUrl":null,"url":null,"abstract":"A compound was isolated from ethyl acetate leaf extract of Caesalpinia bonducella (L.) Flem. and identified as ά-(2-hydroxy-2methylpropyl)-ω-[2-hydroxy-3-methylbut-2-en-1-yl] polymethylene by UV, IR, NMR, HPLC and GC/MS and evaluated for antifungal efficacy by Agar and broth dilution methods against clinical dermatophytes (viz., Trichophyton tonsurans, Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum gypseum and Epidermophyton floccosum). In agar dilution method, T. tonsurans was inhibited only at 400 μg/ml concentration of the compound, whereas all others were inhibited even at low concentrations; optimum pH was 6.0; size of the colonies of the fungi deceased when inoculum size decreased from 10 to 10 spores/ml with increase with compound concentration from 100 to 400 μg/ml. In broth dilution method, mycelial dry weight of all test fungi decreased with the increase in the concentration of the compound; optimum pH was 6.5; decline in biomass of all fungi along with increase in concentration of the compound were observed which were significantly different (p<.05). The dermatophytes used in this study are the most common and widespread of this group and since, there is no scientific evidence to support the medical use of ά-(2-hydroxy-2-methylpropyl)-ω-[2-hydroxy-3-methylbut-2-en-1-yl] polymethylene, further studies are needed in order to elucidate the mechanism(s) of action of these compounds and their derivatives, as well as the antimicrobial activity against other microbial strain in particularly antibiotic resistant dermatophytes.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Evaluation of antidermatophytic activity of ά-(2-hydroxy-2-methylpropyl)-ω-[2-hydroxy-3-methylbut-2-en-1-yl] polymethylene - the compound isolated from Caesalpinia bonducella (L.) Flem.\",\"authors\":\"K. Sagar, G. Vidyasagar\",\"doi\":\"10.5580/19cf\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A compound was isolated from ethyl acetate leaf extract of Caesalpinia bonducella (L.) Flem. and identified as ά-(2-hydroxy-2methylpropyl)-ω-[2-hydroxy-3-methylbut-2-en-1-yl] polymethylene by UV, IR, NMR, HPLC and GC/MS and evaluated for antifungal efficacy by Agar and broth dilution methods against clinical dermatophytes (viz., Trichophyton tonsurans, Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum gypseum and Epidermophyton floccosum). In agar dilution method, T. tonsurans was inhibited only at 400 μg/ml concentration of the compound, whereas all others were inhibited even at low concentrations; optimum pH was 6.0; size of the colonies of the fungi deceased when inoculum size decreased from 10 to 10 spores/ml with increase with compound concentration from 100 to 400 μg/ml. In broth dilution method, mycelial dry weight of all test fungi decreased with the increase in the concentration of the compound; optimum pH was 6.5; decline in biomass of all fungi along with increase in concentration of the compound were observed which were significantly different (p<.05). The dermatophytes used in this study are the most common and widespread of this group and since, there is no scientific evidence to support the medical use of ά-(2-hydroxy-2-methylpropyl)-ω-[2-hydroxy-3-methylbut-2-en-1-yl] polymethylene, further studies are needed in order to elucidate the mechanism(s) of action of these compounds and their derivatives, as well as the antimicrobial activity against other microbial strain in particularly antibiotic resistant dermatophytes.\",\"PeriodicalId\":22514,\"journal\":{\"name\":\"The Internet journal of microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Internet journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5580/19cf\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/19cf","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

从山葵叶乙酸乙酯提取物中分离得到一个化合物。Flem。通过UV、IR、NMR、HPLC和GC/MS鉴定为α -(2-羟基-2-甲基丙基)-ω-[2-羟基-3-甲基-2-烯-1-基]聚乙烯,并通过琼脂和肉汤稀释法评价其对临床皮肤真菌(即:毛癣毛癣菌、mentagrophytes毛癣菌、rubrum毛癣菌、gypseum小孢子菌和絮状表皮癣菌)的抑菌效果。琼脂稀释法中,仅在浓度为400 μg/ml时,该化合物对褐斑单胞菌有抑制作用,而其他化合物在低浓度下均有抑制作用;最适pH为6.0;当接种量从10 ~ 10个孢子/ml,随着化合物浓度从100 ~ 400 μg/ml的增加,菌落死亡。在肉汤稀释法中,所有试验真菌的菌丝干重都随着化合物浓度的增加而降低;最适pH为6.5;随着化合物浓度的增加,真菌生物量呈下降趋势,差异有统计学意义(p< 0.05)。本研究中使用的皮肤植物是这一群体中最常见和最广泛的,由于没有科学证据支持α -(2-羟基-2-甲基丙基)-ω-[2-羟基-3-甲基丁-2-烯-1-基]聚乙烯的医疗用途,因此需要进一步研究以阐明这些化合物及其衍生物的作用机制,以及对其他微生物菌株的抗菌活性,特别是抗生素耐药皮肤植物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Evaluation of antidermatophytic activity of ά-(2-hydroxy-2-methylpropyl)-ω-[2-hydroxy-3-methylbut-2-en-1-yl] polymethylene - the compound isolated from Caesalpinia bonducella (L.) Flem.
A compound was isolated from ethyl acetate leaf extract of Caesalpinia bonducella (L.) Flem. and identified as ά-(2-hydroxy-2methylpropyl)-ω-[2-hydroxy-3-methylbut-2-en-1-yl] polymethylene by UV, IR, NMR, HPLC and GC/MS and evaluated for antifungal efficacy by Agar and broth dilution methods against clinical dermatophytes (viz., Trichophyton tonsurans, Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum gypseum and Epidermophyton floccosum). In agar dilution method, T. tonsurans was inhibited only at 400 μg/ml concentration of the compound, whereas all others were inhibited even at low concentrations; optimum pH was 6.0; size of the colonies of the fungi deceased when inoculum size decreased from 10 to 10 spores/ml with increase with compound concentration from 100 to 400 μg/ml. In broth dilution method, mycelial dry weight of all test fungi decreased with the increase in the concentration of the compound; optimum pH was 6.5; decline in biomass of all fungi along with increase in concentration of the compound were observed which were significantly different (p<.05). The dermatophytes used in this study are the most common and widespread of this group and since, there is no scientific evidence to support the medical use of ά-(2-hydroxy-2-methylpropyl)-ω-[2-hydroxy-3-methylbut-2-en-1-yl] polymethylene, further studies are needed in order to elucidate the mechanism(s) of action of these compounds and their derivatives, as well as the antimicrobial activity against other microbial strain in particularly antibiotic resistant dermatophytes.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
A Case Of Fatal Diphtheria In A Paediatric Patient Identification and Characterization of a Putative Chemotaxis Protein, CheY, from the Oral Pathogen Campylobacter rectus. Oro-Pharyngeal Carriage And Antimicrobial Susceptibility Of Streptococcus Pneumoniae From Healthy Children Isolation, Electron Microscopy And Physicochemical Characterization Of A Brucellaphage Against Brucella Abortus Vaccine Strain S19 Comparison Of Prevalence And Antimicrobial Sensitivity Of Salmonella typhimurium In Apparently Healthy Cattle And Goat In Sango-Ota, Nigeria
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1