黑曲霉利用天然和预处理的木质纤维素废料生产纤维素酶

A. Sridevi, G. Narasimha, B. R. Reddy
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引用次数: 23

摘要

黑曲霉(Aspergillus niger)在Czapek-dox培养基上的生长,该培养基中添加了天然木质纤维素,如锯末、麦秸、甘蔗秸秆和米糠,用于黑曲霉生产纤维素酶。在这些含有0.5%麦秸的Czapek-dox培养基中,培养14天后产生更多的纤维素酶,FPase (2.9 U/ml), CMCase (2.9 U/ml)和β-葡萄糖苷酶(0.93 U/ml)。预处理后的木质纤维素提高了纤维素酶的产量。在处理过的培养基中,添加0.5%锯末的Czapek-dox培养基在培养7天后产生了6.3 U/ml的FPase, CMCase (7.2 U/ml),添加米糠的培养基在培养21天后产生了1.04 U/ml的β-葡萄糖苷酶。
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Production of Cellulase by Aspergillus niger on natural and pretreated lignocellulosic wastes
Growth of Aspergillus niger on Czapek-dox medium supplemented with native lignocellulosics like Sawdust, wheat straw, Sugarcane baggage and Ricebran used for the production of cellulase by A.niger. Of these Czapek-dox medium with 0.5% Wheat straw had yielded more cellulase, FPase (2.9 U/ml), CMCase (2.9 U/ml) and β-glucosidase (0.93 U/ml) after 14-days of incubation. The lignocellulosics after pre-treatment improved cellulase production. Among the treated substrates, Czapek-dox medium with 0.5% saw dust had yielded FPase (6.3 U/ml), CMCase (7.2 U/ml) after 7-days of incubation and the medium with treated rice bran produced β-glucosidase of 1.04 U/ml after 21-days of incubation.
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