David M. Greenberg, Bui-Duy Tam, Eduard Jenny, Benjamin Payes
{"title":"小牛胸腺高纯度二氢叶酸还原酶","authors":"David M. Greenberg, Bui-Duy Tam, Eduard Jenny, Benjamin Payes","doi":"10.1016/0926-6593(66)90035-X","DOIUrl":null,"url":null,"abstract":"<div><p>A procedure has been developed for the preparation of essentially pure dihydrofolate reductase (5,6,7,8-tetrahydrofolate: NAD(P)<sup>+</sup> oxidoreductase, EC 1.5.1.3) from calf-thymus glands. The following criteria of purity were observed: The preparation exhibited only a single protein component by sedimentation ultracentrifugation, sedimentation equilibrium, sucrose gradient centrifugation, and Sephadex chromatography; with a mean mol. wt. of 33 500.</p><p>Electrophoresis on acrylamide gel showed only a single boundary. The pH-activity curve, relative substrate specificity, Michaelis constants of the substrates, and the SH character of the enzyme have been determined. Monovalent cations and Mg<sup>2+</sup> enhanced the enzyme activity. Other divalent cations and sulfhydryl reagents were inhibitory. No stimulation of enzyme activity could be obtained by organic mercurials or urea.</p></div>","PeriodicalId":100160,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1966-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6593(66)90035-X","citationCount":"22","resultStr":"{\"title\":\"Highly purified dihydrofolate reductase of calf thymus\",\"authors\":\"David M. Greenberg, Bui-Duy Tam, Eduard Jenny, Benjamin Payes\",\"doi\":\"10.1016/0926-6593(66)90035-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A procedure has been developed for the preparation of essentially pure dihydrofolate reductase (5,6,7,8-tetrahydrofolate: NAD(P)<sup>+</sup> oxidoreductase, EC 1.5.1.3) from calf-thymus glands. The following criteria of purity were observed: The preparation exhibited only a single protein component by sedimentation ultracentrifugation, sedimentation equilibrium, sucrose gradient centrifugation, and Sephadex chromatography; with a mean mol. wt. of 33 500.</p><p>Electrophoresis on acrylamide gel showed only a single boundary. The pH-activity curve, relative substrate specificity, Michaelis constants of the substrates, and the SH character of the enzyme have been determined. Monovalent cations and Mg<sup>2+</sup> enhanced the enzyme activity. Other divalent cations and sulfhydryl reagents were inhibitory. No stimulation of enzyme activity could be obtained by organic mercurials or urea.</p></div>\",\"PeriodicalId\":100160,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1966-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6593(66)90035-X\",\"citationCount\":\"22\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/092665936690035X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/092665936690035X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Highly purified dihydrofolate reductase of calf thymus
A procedure has been developed for the preparation of essentially pure dihydrofolate reductase (5,6,7,8-tetrahydrofolate: NAD(P)+ oxidoreductase, EC 1.5.1.3) from calf-thymus glands. The following criteria of purity were observed: The preparation exhibited only a single protein component by sedimentation ultracentrifugation, sedimentation equilibrium, sucrose gradient centrifugation, and Sephadex chromatography; with a mean mol. wt. of 33 500.
Electrophoresis on acrylamide gel showed only a single boundary. The pH-activity curve, relative substrate specificity, Michaelis constants of the substrates, and the SH character of the enzyme have been determined. Monovalent cations and Mg2+ enhanced the enzyme activity. Other divalent cations and sulfhydryl reagents were inhibitory. No stimulation of enzyme activity could be obtained by organic mercurials or urea.
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