S. Torabi, N. Abbaspour, F. Rahmani, N. Mohammadkhani
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引用次数: 1
摘要
葡萄藤被归类为对盐度中等敏感的植物。用不同浓度的氯化钾(0.1、0.3、0.5、1、5、10 mM)和NaCl(0、25、50、100 mM NaCl)对3个基因型葡萄(Vitis vinifera L.)进行水培处理。盐胁迫下,各基因型植株各器官Cl-和Na+含量均显著升高。本研究通过盐胁迫下的筛选实验,选择了敏感型(‘GhezelUzum’)、耐型(‘Gharashani’)和半耐型(‘Chawga’)葡萄基因型。与敏感品种相比,‘Gharashani’积累了更高的Na+和Cl- in。即使在高盐度条件下,‘Chawga’根系和茎部也积累了与Na+相似的高K+。对‘Chawga’根系和地上部吸收K+和Na+的Km计算表明,K+和Na+通过根系竞争进入植株。胁迫条件下,两种KUP/KT/ hak型钾转运蛋白在葡萄体内高表达。VvK1.1可能在K+向葡萄组织的加载中起主要作用。不同KCl浓度盐胁迫下,‘Chawga’基因型根系中VvKUP1、VvKUP2转运体和VvK1.1通道的表达显著增加(P < 0.05)。我们的研究结果显示耐盐基因型和敏感基因型之间存在显著差异,并强调了特定转录物的积累与耐盐程度之间的密切关系。
Effects of salinity on potassium absorption and expression of K+transporter genes at different concentrations of potassium in Grape (Vitis vinifera L.)
Grapevine is classified as a moderately sensitive plant to salinity. Hydroponically three grape genotypes (Vitis vinifera L.) were treated with different concentrations of KCl (0.1, 0.3, 0.5, 1, 5, 10 mM KCl) and NaCl (0, 25, 50,100 mM NaCl). Cl- and Na+ contents were significantly increased in different plant organs of all the genotypes under salinity. In this study, sensitive ('GhezelUzum'), tolerant ('Gharashani') and semi-resistant ('Chawga') grape genotypes were selected based on screening experiments under salinity. 'Gharashani' accumulated higher Na+ and Cl- in roots compared to the sensitive one. 'Chawga' accumulated high K+ similar to Na+ in root and shoot even at high salinity. Km calculation for K+ and Na+ uptake in root and shoot of 'Chawga' showed that K+ and Na+ compete to enter the plant through roots. Two KUP/KT/HAK-type potassium transporters are expressed highly in the grapevine during stress. VvK1.1 could play a major role in K+ loading into grape tissues. The expression of VvKUP1 and VvKUP2 transporters and VvK1.1 channel in roots of 'Chawga' genotype increased significantly (P < 0.05) at different KCl concentrations under salinity stress. Our results showed a significant difference between tolerant and sensitive genotypes and highlighted a strong relationship between the accumulation of specific transcripts and the degree of salinity tolerance.