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The interplay between hormone signaling and defense gene expression in grapevine genotypes carrying genetic resistance against Plasmopara viticola 葡萄抗浆原病基因型中激素信号与防御基因表达的相互作用
Pub Date : 2021-10-26 DOI: 10.5073/VITIS.2021.60.195-206
M. D. Rossarolla, T. C. Tomazetti, L. Welter, H. P. Santos, M. Stefanini, O. Trapp, M. P. Guerra, R. Nodari
The present study aimed to investigate plant defense related pathways during Plasmopara viticola infection in Vitis vinifera varieties. Plant material consisted of 'Chardonnay' (no Rpv), 'Regent' (Rpv3-1), 'Bronner' (Rpv3-3+Rpv10), 'Calardis Blanc' (Rpv3-1+Rpv3-2), and the breeding selection GF15 (Rpv1+Rpv3-1). Gene expression analysis was carried out for the varieties 'Regent', GF15, 'Bronner', and 'Chardonnay'. Hormonal quantification was performed for jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA), indole-3-acetic acid (IAA), and trans-zeatin-ribose (tZR). The samples were collected from plants cultivated in vitro inoculated with Plasmopara viticola sporangia, and collected at 0, 1-, 3-, 5-, and 7-days post inoculation (DPI) for gene expression; and 0, 3, 5, and 7 DPI for hormonal quantification. The results showed an interaction between genotype and time post inoculation in gene expression and hormonal pathways linked with pathogen recognition. Both jasmonate and salicylic acids were involved in the resistance response. The role of stilbenes acting against the pathogen at different times was also confirmed. Changes in the expression of genes linked to cell defense were observed in all evaluated genotypes; however, genotypes with R-loci responded more quickly than the variety without R-loci, activating mechanisms of cell death, resulting in symptoms of hypersensitivity.
本研究旨在探讨葡萄品种侵染葡萄浆原菌过程中植物防御的相关途径。植株材料包括‘霞多丽’(不含Rpv)、‘摄政’(Rpv3-1)、‘布朗纳’(Rpv3-3+Rpv10)、‘Calardis Blanc’(Rpv3-1+Rpv3-2)和选材GF15 (Rpv1+Rpv3-1)。对‘Regent’、GF15、‘Bronner’和‘Chardonnay’进行了基因表达分析。测定茉莉酸(JA)、水杨酸(SA)、脱落酸(ABA)、吲哚-3-乙酸(IAA)和反式玉米蛋白核糖(tZR)的激素含量。样品采集自离体接种葡萄浆原菌孢子囊的植株,分别于接种后0、1、3、5、7 d采集,用于基因表达;0、3、5和7 DPI用于激素定量。结果表明,与病原菌识别相关的基因表达和激素通路的基因型与接种后时间存在交互作用。茉莉酸和水杨酸都参与了抗性反应。二苯乙烯在不同时间对病原菌的作用也得到了证实。在所有评估的基因型中都观察到与细胞防御相关的基因表达的变化;然而,含有r -基因座的基因型比没有r -基因座的品种反应更快,激活细胞死亡机制,导致过敏症状。
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引用次数: 1
The impact of temperature on 'Pinot Noir' berry and wine quality in a steeply sloping cool climate vineyard in South Australia 在南澳大利亚一个陡坡的凉爽气候葡萄园中,温度对“黑皮诺”浆果和葡萄酒品质的影响
Pub Date : 2021-10-26 DOI: 10.5073/VITIS.2021.60.169-178
Gavin Duley
Viticulture is particularly sensitive to climate change, as temperature is critical to the two key concepts of terroir and vintage. Vitis vinifera L. 'Pinot Noir' is known to not grow well in hot climates. A trial was run over two years in a commercial vineyard in the Adelaide Hills, South Australia, to determine the impact of higher temperatures on the 'Pinot Noir' grape and wine colour. A factorial experiment combining two sources of variation in temperature was established: three positions on a steep vineyard slope, and two thermal treatments: unheated control and heated with passive open-top transparent chambers. Elevated temperature decoupled sugars and anthocyanins in grape berries, with the heated treatment grapes producing a lower anthocyanin concentration for the same concentration of total soluble solids. Temperature effects were less defined for small batches of wines prepared from these grapes, with the wines from heated vines having higher total phenolics, and perhaps consequently lower CIELab b* values (i.e., less blue pigmentation/anthocyanins). The study provides a unique insight into temperature gradients on a steeply sloping site and the effects on colour development of 'Pinot Noir' grape berries and wine.
葡萄栽培对气候变化特别敏感,因为温度对风土和年份这两个关键概念至关重要。葡萄属葡萄众所周知,黑皮诺在炎热的气候下生长不好。在南澳大利亚阿德莱德山的一个商业葡萄园进行了为期两年的试验,以确定高温对“黑皮诺”葡萄和葡萄酒颜色的影响。建立了一个结合两个温度变化源的析因实验:在陡峭的葡萄园斜坡上的三个位置,以及两种热处理:不加热控制和被动开顶透明室加热。升高的温度使葡萄果实中的糖和花青素分离,在相同浓度的总可溶性固体中,加热的葡萄产生较低的花青素浓度。用这些葡萄酿制的小批量葡萄酒的温度效应不太明确,从加热的葡萄酿制的葡萄酒具有更高的总酚类物质,因此可能会降低CIELab b*值(即较少的蓝色色素/花青素)。该研究提供了一个独特的视角,了解陡坡场地的温度梯度以及对“黑皮诺”葡萄浆果和葡萄酒颜色发展的影响。
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引用次数: 0
Physiological changes induced by either pre- or post-veraison deficit irrigation in 'Merlot' vines grafted on two different rootstocks 嫁接在两种不同砧木上的“梅洛”葡萄植株在转代前或转代后亏缺灌溉引起的生理变化
Pub Date : 2021-10-26 DOI: 10.5073/VITIS.2021.60.153-161
G. Palai, R. Gucci, G. Caruso, C. D'onofrio
Reduced summer precipitations and higher evapotranspiration due to elevated temperatures are expected to enhance the impact of water deficit in modern viticulture. We investigated the effect of the timing of deficit irrigation on vine growth, water relations, yield and grape composition in 'Merlot' vines grafted on 1103P or SO4. In both years we did not measure any differences between rootstocks in stem water potential (SWP). Vegetative growth was decreased by the restriction of irrigation between fruit set and veraison. Stomatal conductance (gs) was affected by irrigation, but not by the rootstock. During the pre-veraison period there was a clear inverse relationship between gs and SWP. The leaf non photochemical quenching readily responded to the stress imposed on 1103P rootstock. Vines subjected to water deficit between fruit set and veraison produced smaller berries than well irrigated ones, whereas deficit applied after veraison determined about 10 % differences in berry weight. The highest and lowest values of pH and TA were measured in berries from pre-veraison deficit irrigated vines grafted on both 1103P and SO4, respectively.
由于气温升高,夏季降水减少和蒸散量增加,预计将加剧现代葡萄栽培中水分亏缺的影响。研究了亏缺灌溉时机对嫁接在1103P和SO4上的‘梅洛’葡萄生长、水分关系、产量和葡萄成分的影响。在这两年中,我们都没有测量到不同砧木在茎水势(SWP)方面的差异。坐果至花期限灌,营养生长受到抑制。灌水对气孔导度有影响,而对砧木没有影响。在变前期,gs与SWP呈明显的负相关。叶片非光化学猝灭对施加在1103P砧木上的胁迫有明显的响应。在坐果期和灌浆期之间缺水的葡萄藤比灌浆后缺水的葡萄藤结出的果实要小,而灌浆后缺水的葡萄藤的果实重量差异约为10%。在1103P和SO4两种处理下,分别测定了旱前亏缺灌溉葡萄藤的果实pH和TA的最高和最低值。
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引用次数: 8
Three-dimensional approach for identification of red grape cultivars by fingerprint of wine anthocyanins 葡萄酒花青素指纹图谱三维鉴别红葡萄品种方法研究
Pub Date : 2021-10-26 DOI: 10.5073/VITIS.2021.60.163-168
N. Shakulashvili, T. Chavchanidze, N. Ksovreli
The authenticity of grape cultivars is an important tool for wine controlling systems. The identification of red grape cultivars by wine anthocyanin spectra is a well-known method, but the analysis of applied parameters does not produce exact results. This work aimed at identifying substantive parameters, which, if applied simultaneously, will allow identifying numerous cultivars with high certainty. Liquid chromatography has been applied to obtain anthocyanin profiles of single-cultivar red wines made of various cultivars. Ratios of the peak areas of individual anthocyanins were identified. The proportions of anthocyanins depend on many factors, but their constancy is predetermined genetically. Certain ratios of peak areas of individual anthocyanins can be estimated as constant and typical for concrete cultivars. Revealed combinations of three ratios were estimated as unique for each cultivar. Moreover, different clones of certain cultivars have different combinations of constant ratios. Analysed samples were distributed in groups according to the combination of constancies of three ratios manifested in chromatographic peaks. The three-dimensional model produced allows data distribution in different groups. Each group corresponds to individual cultivars or different clones of one cultivar. Their belonging to concrete groups makes it possible to efficiently identify unknown single-cultivar wines and detect forgery.
葡萄品种的真实性是葡萄酒控制系统的重要工具。葡萄酒花青素光谱鉴别红葡萄品种是一种公认的方法,但对应用参数的分析并不能得出准确的结果。这项工作旨在确定实质性参数,如果同时应用这些参数,将能够高度确定地识别许多品种。采用液相色谱法对不同品种的单品种红葡萄酒进行了花青素谱分析。确定了各个花青素的峰面积比值。花青素的比例取决于许多因素,但它们的稳定性是由基因决定的。个别花青素峰面积的一定比例可以估计为恒定和典型的具体品种。结果表明,三个比例的组合对每个品种来说都是独一无二的。此外,某些品种的不同无性系具有不同的恒比组合。根据色谱峰中所显示的三个比值的常数组合,将分析样品分组分布。生成的三维模型允许数据在不同组中分布。每个类群对应于单个品种或一个品种的不同无性系。它们属于具体的群体,使得有效地识别未知的单一品种葡萄酒和检测伪造成为可能。
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引用次数: 0
Global warming allows two grape crops a year, with about two months apart in ripening dates and with very different grape composition - The forcing vine regrowth to obtain two crops a year 全球变暖使得葡萄一年可以收获两次,成熟期相差两个月,葡萄的成分也大不相同——这迫使葡萄树一年要再生两次
Pub Date : 2021-07-12 DOI: 10.5073/VITIS.2021.60.119-124
F. Toda
With the aim of delaying the ripening of grapes by around two months, a technique has been proposed based on forcing vine regrowth (Gu et al. 2012, Martinez de Toda et al. 2019). It is a bold method to fight against climate warming that could be only developed in really warm viticultural regions. It consists in shortening the growing shoots to several nodes with the aim of forcing vine regrowth; in order to force budbreak, shoot regrowth, and cropping, the source of inhibition needs to be eliminated and to this end, lateral shoots, leaves, and primary clusters, if they exist, are removed.But the main drawback of the forcing vine regrowth technique is loss of yield. In order to avoid this loss of yield and not to eliminate the primary clusters already formed in the main shoots, it is possible to force the development of buds of the fifth and sixth nodes, but maintaining the clusters of the main shoots. In this way, the yield of the forced buds would be added to the normal or primary yield of the shoots. The experiences carried out with 'Grenache', 'Tempranillo' and 'Maturana Tinta' varieties in La Rioja (Spain) are presented.
为了将葡萄成熟期推迟两个月左右,研究人员提出了一种基于强迫葡萄再生的技术(Gu et al. 2012, Martinez de Toda et al. 2019)。这是一种对抗气候变暖的大胆方法,只有在真正温暖的葡萄种植区才能发展。它包括缩短生长的芽到几个节,目的是迫使藤蔓再生;为了迫使发芽、新梢再生和种植,需要消除抑制源,为此目的,去除侧芽、叶片和初级簇,如果它们存在的话。但强迫葡萄再生技术的主要缺点是产量损失。为了避免这种产量损失,并且不消除主枝上已经形成的初级簇,可以强迫第五和第六节的芽发育,但保持主枝的簇。这样,强迫芽的产量将增加到芽的正常或初级产量。介绍了在西班牙拉里奥哈种植“歌海娜”、“丹魄”和“马图拉纳”的经验。
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引用次数: 5
Frateuria defendens reduces yellows disease symptoms in grapevine under field conditions 在田间条件下,枸杞子可减少葡萄黄病症状
Pub Date : 2021-07-12 DOI: 10.5073/VITIS.2021.60.109-117
V. Naor, T. Zahavi, R. Barkai, N. Weiss, Netta Mozes-Daube, O. Dror, C. Finkelstein, S. Aharon, Ofir Bahar, E. Zchori‐Fein, Lilach Iasur-Kruh
Yellows diseases in grapevine, associated with the presence of different phytoplasmas, are a major problem for growers, with no environmentally friendly means of control. Frateuria defendens (Frd), a bacterium with endophytic traits, has been shown to reduce yellows symptoms in grapevine plantlets under laboratory conditions. The objective of this study was to test whether similar effects could be achieved under field conditions. A trial was conducted in a heavily infected vineyard in northern Israel for two consecutive years. A suspension of Frd cells (108·mL-1) was applied bi-weekly by foliar spray on grapevines from bud burst to leaf senescence. Frd penetrated the leaves during the growing period but not during leaf senescence and could be detected in the leaves by PCR analysis up to 14 days post-spraying. The rate of yellows infection was lower in the treated grapevines compared to its increase in untreated grapevines and the yield of symptomatic plants was improved by 10 to 20 %. Taken together, the results suggest Frd acted as a biological control agent in vineyards under the experimental conditions tested.
葡萄黄病与不同植物原体的存在有关,对种植者来说是一个主要问题,没有环境友好的控制手段。防御葡萄球菌(Frd)是一种具有内生特性的细菌,在实验室条件下已被证明可以减少葡萄藤植株的黄色症状。本研究的目的是测试在野外条件下是否能达到类似的效果。在以色列北部一个感染严重的葡萄园里进行了连续两年的试验。以108·mL-1的Frd细胞为悬浮液,每两周喷施一次,从萌发到叶片衰老。喷施后14 d,在叶片中仍可检测到Frd的存在,但在叶片衰老期未检测到。与未处理的葡萄相比,处理过的葡萄的黄病感染率较低,有症状植株的产量提高了10%至20%。综上所述,结果表明,在实验条件下,Frd在葡萄园中起到了生物防治剂的作用。
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引用次数: 1
Erinea in the 'Ansonica' grapevine cultivar: trichome complement, histological effects and analysis of chlorophyll fluorescence in affected leaves 葡萄品种“安尼卡”的毛癣:毛体补体、组织学效应和叶片叶绿素荧光分析
Pub Date : 2021-07-12 DOI: 10.5073/VITIS.2021.60.101-108
M. Fambrini, M. Landi, C. Pugliesi
Grapevine leaves are usually characterized by trichomes, specialized epidermal cells. They are interesting in ampelography and also important for the plant ecological responses in biotic and abiotic interactions. In nature, the trichome development is a genetic trait but it can be modified by pests as eriophyid mites. Colomerus vitis is quite common and its economic value is sometime substantial. Here, we studied the leaf erineum induced by C. vitis on 'Ansonica' ('Inzolia'), an important grapevine cultivar characterized by a low level of leaf trichome coating. To date, the interaction between C. vitis and grape has been investigated in few pedo-climatic conditions and no data are reported in 'Ansonica'. Therefore, our objectives were: (1) the analysis, in a Tuscan environment, of the morphology and histology of trichomes in 'Ansonica' leaves unaffected or affected by C. vitis; (2) evaluation, in mature leaves, of the effects of the mite both on pigment content and chlorophyll a fluorescence parameters. 'Ansonica' was devoid of glandular trichomes but it has been established the presence of few simple trichomes strictly associated with the veins. In the erineal sectors, a dense proliferation of simple trichomes in the abaxial epidermis and the development of hyperplasia in the adaxial surface were observed. Moreover, the leaf sections in the erineal regions were thicker due to an abnormal development of the lacunar parenchyma, and trichome proliferation was also extended to interveinal regions. Leaves with erinea showed a deficient content of carotenoids, in comparison to unaffected leaves. In 'Ansonica' leaves, C. vitis induced a decrease in the steady-state operational efficiency of photosystem II associated to a reduction in photochemical quenching and an increase in non-photochemical quenching values. In leaves with erinea, the reduction of photosystem II efficiency was extended to foliar areas not directly affected by galls. The collected results highlight that 'Ansonica' is susceptible to attacks by C. vitis and in the case of widespread leaf attacks the productive damage should not be underestimated.
葡萄藤的叶子通常以毛状体和特化的表皮细胞为特征。它们对植物在生物和非生物相互作用中的生态响应也很重要。在自然界中,毛状体的发育是一种遗传性状,但它可以被害虫如叶面螨所改变。葡萄花柱很常见,其经济价值有时是可观的。本文研究了葡萄球菌(C. vitis)对‘anonica’('Inzolia')叶片的诱导作用,anonica是一种重要的葡萄品种,其叶片毛状膜覆盖水平较低。迄今为止,葡萄球菌与葡萄之间的相互作用已经在少数土壤气候条件下进行了研究,但在“anonica”中没有报道数据。因此,我们的目标是:(1)在托斯卡纳环境中分析未受葡萄球菌感染或影响的“anonica”叶片中毛状体的形态和组织学;(2)评价螨对成熟叶片色素含量和叶绿素a荧光参数的影响。' anonica '是缺乏腺毛,但它已经建立了一些简单的毛与静脉严格相关的存在。在阴部,观察到单毛在背面表皮密集增生,在正面表面增生。阴部的叶切片由于腔隙薄壁组织的异常发育而变厚,毛状体增生也扩展到脉间区。与未受影响的叶子相比,有雌蕊的叶子显示出类胡萝卜素含量不足。在' anonica '叶片中,C. vitis诱导光系统II稳态运行效率的降低,这与光化学猝灭值的减少和非光化学猝灭值的增加有关。在有雌蕊的叶片中,光系统II效率的降低扩展到不受瘿瘤直接影响的叶区。收集的结果突出表明,' anonica '易受葡萄球菌的攻击,在广泛的叶片攻击的情况下,生产损害不应低估。
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引用次数: 0
Pruning cuts affect wood necrosis but not the percentage of budburst or shoot development on spur pruned vines for different grapevine varieties 对不同品种的葡萄品种,剪枝对树干坏死有影响,但对芽、芽发育无影响
Pub Date : 2021-07-12 DOI: 10.5073/VITIS.2021.60.137-141
P. Faúndez-López, J. Delorenzo-Arancibia, G. Gutiérrez-Gamboa, Y. Moreno-Simunovic
Two experimental studies were performed in this trial. In the first, the aim was to quantify wood necrosis generated by pruning cuts on aboveground permanent (arms and trunks) and non-permanent (spurs) woody structures of 'Cabernet Sauvignon' vines. In the second, the goal was to evaluate the effect of cutting distance from the basal end of the shoot in spur pruned vines on budburst and further shoot development for 'Grenache', 'Cabernet Franc' and 'Malbec' varieties. Based upon the first experiment, the area and depth of wood necrosis was highly influenced by the distance where the pruning cut was performed over the node. Furthermore, the diameter of the spur that was cut was not significantly related to either the area or the depth of the necrotic wood generated after the cut. Aboveground vine wood necrotic area ranged from 9 to 44 % of the total wood area measured in 'Cabernet Sauvignon' cordon trained spur pruned 25-year-old grapevines. For each vine a larger proportion of the necrotic wood (20 to 46 % of necrotic area) was present in the arms when compared to the trunks (1 to 28 % of necrotic area). As a result of the second experiment, spur budburst and further shoot development was not affected by the distance from the node where the pruning cut was performed for any of the cultivars considered in the study contrary to what is commonly believed.
本试验进行了两项实验研究。首先,我们的目标是量化赤百纳(Cabernet Sauvignon)葡萄的地上永久性(臂和树干)和非永久性(刺)木质结构上的修剪所产生的木材坏死。第二项研究的目标是评估距直枝修剪葡萄藤茎基部的修剪距离对“歌海娜”、“品丽珠”和“马尔贝克”品种的芽和进一步的芽发育的影响。在第一次试验的基础上,木材坏死的面积和深度受到节点上进行修剪的距离的高度影响。此外,被切割的骨刺直径与切割后产生的坏死木材的面积或深度均无显著相关。在“赤霞珠”(Cabernet Sauvignon)用栅栏修剪过的25岁葡萄藤中,地上葡萄藤木材坏死面积占总木材面积的9%至44%。与树干(坏死面积的1 - 28%)相比,每根藤的枝部坏死木材的比例(坏死面积的20% - 46%)更大。第二次试验的结果表明,与通常认为的相反,在研究中考虑的任何品种中,距修剪节点的距离不影响骨刺芽和进一步的芽发育。
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引用次数: 7
Global transcriptome analysis of heat stress response of grape variety 'Fantasy Seedless' under different irrigation regimens 不同灌溉方式下无籽葡萄品种‘幻想’热胁迫响应的转录组分析
Pub Date : 2021-07-12 DOI: 10.5073/VITIS.2021.60.143-151
A. Upadhyay, A. Upadhyay
Grapevine (Vitis vinifera L.), a commercially important fruit crop worldwide, faces several challenging conditions during its growth cycle. Among many abiotic stresses, heat and moisture stresses have major impact on grapevine productivity and fruit quality. Transcriptome analysis of heat stress response of grape variety 'Fantasy Seedless' grown under different irrigation regimens identified large number of differentially expressed genes. Genes belonging to chaperone mediated protein folding and cell-wall modification pathways were found to play a significant role in plant response to heat as well as moisture stress. Subsurface irrigation helped minimize the adverse effects of stress through modulation of genes involved in cell homeostasis. The study has given critical insight into grapevine response to heat stress arising due to aberrant weather conditions.
葡萄藤(Vitis vinifera L.)是一种全球重要的商业水果作物,在其生长周期中面临着几个具有挑战性的条件。在众多非生物胁迫中,湿热胁迫对葡萄产量和果实品质影响最大。通过对不同灌溉条件下无籽葡萄品种‘幻想无籽’热胁迫响应的转录组分析,发现了大量差异表达基因。研究发现,伴侣蛋白介导的蛋白质折叠和细胞壁修饰途径的基因在植物对高温和水分胁迫的响应中起着重要作用。地下灌溉通过调节参与细胞稳态的基因,帮助减少应激的不利影响。这项研究为葡萄藤对异常天气条件引起的热应激的反应提供了关键的见解。
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引用次数: 1
Texture properties and phenol extractability indices of the grape berry under pre- and post-veraison water deficit (Vitis vinifera L. 'Kékfrankos') 改良前后水分亏缺条件下葡萄果实的质地特性和酚提取性指标。“Kekfrankos”)
Pub Date : 2021-07-12 DOI: 10.5073/VITIS.2021.60.125-135
Zs. Zsófi, X. Pálfi, Sz. Villangó
Optimal water supply is one of the most important factors in quality wine making. However, water availability is limited in several wine regions and water shortage is getting even worse due to climate change, especially under arid climate conditions. Therefore, proper water management of the vineyards, the amount and the timing of irrigation will play a crucial role in sustainable viticulture in the near future. In this study, the effect of timing of moderate water deficit on berry texture characteristics and phenolic maturity were investigated. 'Kekfrankos' grapevines were submitted to different water regimes: moderate water deficit from berry set until veraison (WD1), moderate water deficit from veraison until harvest (WD2), no water deficit (C). Concentration of the phenolic components of the grape berry skins and berry mixture for the Glories indices were measured by spectrophotometer (UVmini-1240 CE UV-VIS, Shimadzu, Japan). Cell and seed maturity indices (CMI%, SMI%) were also calculated. Berry texture characteristics were monitored by a TA.XT Plus Texture Analyser (Stable Micro System, UK). Berry skin and seed texture properties were affected by water regimes. Skin and seed hardness of WD1 were significantly lower than those of WD2. Significant differences were found in berry hardness between the treatments (C>WD1>WD2). Berry gumminess, resilience and chewiness of WD2 were lower compared to C and WD1. Anthocyanin extractability was higher when plants were not subjected to post-veraison water limitations (WD1). Furthermore, the seed maturity index was lower when water deficit occurred between berry set and veraison. Pre-veraison water deficit resulted in delayed ripening, softer seeds and thus higher phenol extractability compared to WD2 and C. Late seasonal water deficit resulted in thicker skins, which was accompanied by lower anthocyanin extractability than in WD1 and C. It seems that anthocyanin extractability is influenced by the actual water status of the grapevine rather than skin textural properties. However, there are some connections between skin texture parameters and maturity indices.
最佳供水是保证葡萄酒质量的重要因素之一。然而,在一些葡萄酒产区,水资源供应有限,而且由于气候变化,特别是在干旱气候条件下,水资源短缺问题变得更加严重。因此,在不久的将来,适当的葡萄园水管理、灌溉的数量和时间将在可持续葡萄栽培中发挥至关重要的作用。本研究研究了适度亏水时机对浆果质地特征和酚类成熟度的影响。将‘Kekfrankos’葡萄植株进行不同的水分处理:从果实结实到果实成熟(WD1)中度水分亏缺,从果实成熟到收获(WD2)中度水分亏缺,无水分亏缺(C)。用分光光度计(UVmini-1240 CE UV-VIS, Shimadzu, Japan)测量葡萄浆果皮和浆果混合物中酚类成分的浓度。计算细胞和种子成熟度指数(CMI%, SMI%)。用TA监测浆果的质地特征。XT Plus纹理分析仪(英国稳定微系统公司)。浆果果皮和种子的质地特性受水分状况的影响。WD1的果皮和种子硬度显著低于WD2。不同处理间浆果硬度差异显著(C>WD1>WD2)。与C和WD1相比,WD2的浆果黏性、弹性和咀嚼性较低。不受改良后水分限制(WD1)时,花青素提取率较高。结果表明,坐果期和转代期水分亏缺导致种子成熟指数降低。与WD1和c相比,催熟前水分不足导致成熟延迟,种子更软,因此苯酚的可提取性更高。季节后期水分不足导致果皮更厚,同时花青素的可提取性低于WD1和c。似乎花青素的可提取性受葡萄实际水分状况而非果皮质地特性的影响。然而,皮肤纹理参数与成熟度指标之间存在一定的联系。
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引用次数: 1
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Vitis: Journal of Grapevine Research
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