桄榔子叶瘿蛋白及同工酶的电泳图谱

R. Choudhary, Sanjay Kumar, B. Kumar, Devendra Kumar
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引用次数: 0

摘要

采用电泳方法研究了红杉叶瘿中蛋白质和同工酶(过氧化物酶和多酚氧化酶)的含量。结果表明,叶片瘿与正常叶片的sds蛋白和同工酶活性带型存在一定差异。剥蚀叶片样品每行共观察到20条条带,而实验植物正常叶片粗蛋白谱图则少观察到1条条带。我们观察到,与正常叶片相比,剥蚀叶片中蛋白质带的强度更高。多酚氧化酶和过氧化物酶活性在剥蚀叶片中比在正常叶片中高。
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Electrophoretic Profile of Protein and Isozymes in Leaf Gall Of Pongamia Pinnata (L.)
In the present investigation the electrophoretic studies of protein and isozymes (peroxidase and polyphenol oxidase) in leaf galls of Pongamia pinnata induced by Aceria pongamiae were carried out. The results showed some variation in SDS-protein and isozymes activities banding patterns among leaf galls and normal leaf. Total twenty bands were observed in each lane of galled leaf sample, in comparison one less band was observed in normal leaf crude protein profiling of experimental plant. We observed that the intensity of protein bands was higher in galled leaf as compare to normal leaf protein. Intensity of polyphenol oxidase and peroxidase activity was found to be very high in galled leaves as compared to their normal counterparts.
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