寄生兰提取物酚类含量、类黄酮含量及抗氧化能力的研究

Nurul Fatihah Muhamad Pithonah, Mohd Adzim Khalili Rohin, Nor Aishah Fatiha Jailani, N. Ridzwan, Mimie Noratiqah Jumli, Norhayati Abd Hadi, Ruziana Ishak, Atif Amin Baig
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摘要

寄生Lansium parasiticum (Osbeck)或其同义词Epicharis parasitica来自Meliaceae家族,传统上用于各种医学应用。本研究旨在测定不同提取物的总酚含量、总黄酮含量及抗氧化活性。采用50%乙醇、100%水、100%丙酮和100%氯仿四种不同的溶剂提取杜库肉。采用福林-环钙铝-氯化铝比色法测定总酚和类黄酮含量。DPPH法和ABTS法评价其抗氧化活性。结果表明,50%乙醇提取率最高,为10.81%±0.004%。水提物总酚含量最高,为152.910 mg GAE/100 g±22.143,总黄酮含量为1669.723±370.091 mg QE/100 g,抗氧化活性DPPH为68.51%±2.730,ABTS为6.063 U/ml±0.721。总酚含量与ABTS的相关性(r = 0.719, p = 0.029)有统计学意义。因此,这种水果的提取物在未来的食品工业中作为廉价的天然抗氧化剂有很大的潜力。研究人员将在未来的体外或体内生物学研究中进一步研究这些结果。
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Study on phenolic content, flavonoid content, and antioxidant capacity of extracts from Lansium parasiticum (Osbeck)
Lansium parasiticum (Osbeck) or its synonym Epicharis parasitica is from the Meliaceae family and has traditionally been used for various medical applications. This study aimed to determine the total phenolic content, total flavonoid content, and antioxidant activity of different duku extracts. A total of four different solvents, 50% ethanol, 100% aqueous, 100% acetone, and 100% chloroform, were used to extract duku flesh. The total phenolic and flavonoid content was determined using the Folin-Ciocaltealuminumminium chloride colorimetric method. The DPPH and ABTS method evaluated the antioxidant activity. The result showed that 50% ethanol presented the highest extraction yield, 10.81% ± 0.004. It is also revealed that aqueous extract exhibited the highest amount of total phenolic content of 152.910 mg GAE/100 g ± 22.143, total flavonoid content of 1669.723 ± 370.091 mg QE/100 g, and antioxidant activity DPPH of 68.51 % ± 2.730 and ABTS of 6.063 U/ml ± 0.721 compared to other extracts. The correlation between total phenolic content and ABTS (r = 0.719, p = 0.029) showed a statistically significant result. Therefore, the extracts of this fruit have promising potential as cheap sources of future natural antioxidant agents in the food industry. Researchers will further these results for future in-vitro or in-vivo biological studies.
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