{"title":"Kesan kaedah pengekstrakan fukoidan terhadap hasil, kandungan fukosa dan ketulenan fukoidan daripada Sargassum sp. Dari pulau langkawi, Malaysia","authors":"Bibi Marliana Baba, Wan Mustapha, Seng Joe Lim","doi":"10.17576/MJAS-2018-2201-11","DOIUrl":null,"url":null,"abstract":"The objective of this study was to determine the effect of extraction methods on the yield, fucose content and purity of fucoidan that had been isolated from brown seaweed, Sargassum sp. The extraction of fucoidan was carried out based on the factorial design assigned, which involved three different parameters including two types of extraction (acid and alkaline), three different extraction temperatures (45, 65 and 85 °C) and three different extraction time (1, 3 and 5 hours). Results obtained showed that the best extraction parameter that gave the highest yield of fucoidan extract was the alkaline extraction at 65 °C for 3 hours. Alkaline extraction also produced higher purity and better quality of fucoidan compared to the fucoidan extracted by hydrochloric acidic.","PeriodicalId":22844,"journal":{"name":"The Malaysian Journal of Analytical Sciences","volume":"88 1","pages":"87-94"},"PeriodicalIF":0.0000,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Malaysian Journal of Analytical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17576/MJAS-2018-2201-11","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Kesan kaedah pengekstrakan fukoidan terhadap hasil, kandungan fukosa dan ketulenan fukoidan daripada Sargassum sp. Dari pulau langkawi, Malaysia
The objective of this study was to determine the effect of extraction methods on the yield, fucose content and purity of fucoidan that had been isolated from brown seaweed, Sargassum sp. The extraction of fucoidan was carried out based on the factorial design assigned, which involved three different parameters including two types of extraction (acid and alkaline), three different extraction temperatures (45, 65 and 85 °C) and three different extraction time (1, 3 and 5 hours). Results obtained showed that the best extraction parameter that gave the highest yield of fucoidan extract was the alkaline extraction at 65 °C for 3 hours. Alkaline extraction also produced higher purity and better quality of fucoidan compared to the fucoidan extracted by hydrochloric acidic.