Pub Date : 2020-04-15DOI: 10.17576/mjas-2018-2202-04
Natalia Urzúa, G. Prieto, C. Lüders, M. P. Tonini, E. Picco, C. Errecalde
Marbofloxacin (MBX) is an antimicrobial for exclusive veterinary use, relatively new and of which there are few analytical methods for the extraction, identification and quantification in plasma and edible tissues (muscle, liver, kidney, lung, skin) in broiler chickens. The objective of developing a simple, sensitive and efficient extraction and detection method through highperformance liquid chromatography (HPLC) with fluorescence detection. For the preparation of the samples, known concentrations of MBX, enrofloxacin (ENX) as internal standard, methanol, water and perchloric acid, were added to plasma or tissues. Chromatographic conditions were: fluorescence detector at λex 295 and λem 490 nm, mobile phase composed of deionized water, acetonitrile and triethylamine adjusted to pH 3.0. For all matrices, the method was linear (r= 0.99) for MAR concentrations (0.039 to 2.5 μg/ml or gr), with an average retention time for MBX in the different matrices of 3.97 ± 0.144 minutes, and 4.99 ± 0.24 minutes for the internal standard (enrofloxacin). Detection limit in all matrices was less than 0.006 μg/ml, while recoverability percentage was 89 ± 4.65% and variation coefficients in intraday-interday assay ≤ 1.4%, determining the accuracy of the study. The analytical method applied is efficient, sensitive and reliable for future residue determinations or pharmacokinetic studies of marbofloxacin in plasma, muscle, liver, kidney, skin and lung in broiler chickens.
{"title":"Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues","authors":"Natalia Urzúa, G. Prieto, C. Lüders, M. P. Tonini, E. Picco, C. Errecalde","doi":"10.17576/mjas-2018-2202-04","DOIUrl":"https://doi.org/10.17576/mjas-2018-2202-04","url":null,"abstract":"Marbofloxacin (MBX) is an antimicrobial for exclusive veterinary use, relatively new and of which there are few analytical methods for the extraction, identification and quantification in plasma and edible tissues (muscle, liver, kidney, lung, skin) in broiler chickens. The objective of developing a simple, sensitive and efficient extraction and detection method through highperformance liquid chromatography (HPLC) with fluorescence detection. For the preparation of the samples, known concentrations of MBX, enrofloxacin (ENX) as internal standard, methanol, water and perchloric acid, were added to plasma or tissues. Chromatographic conditions were: fluorescence detector at λex 295 and λem 490 nm, mobile phase composed of deionized water, acetonitrile and triethylamine adjusted to pH 3.0. For all matrices, the method was linear (r= 0.99) for MAR concentrations (0.039 to 2.5 μg/ml or gr), with an average retention time for MBX in the different matrices of 3.97 ± 0.144 minutes, and 4.99 ± 0.24 minutes for the internal standard (enrofloxacin). Detection limit in all matrices was less than 0.006 μg/ml, while recoverability percentage was 89 ± 4.65% and variation coefficients in intraday-interday assay ≤ 1.4%, determining the accuracy of the study. The analytical method applied is efficient, sensitive and reliable for future residue determinations or pharmacokinetic studies of marbofloxacin in plasma, muscle, liver, kidney, skin and lung in broiler chickens.","PeriodicalId":22844,"journal":{"name":"The Malaysian Journal of Analytical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88949505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.17576/MJAS-2019-2305-05
Noorlaila Ahmad, L. Heng, S. A. Hanifah, F. Salam
{"title":"Polymeric films as matrixes for the immobilization of mixed natural dyes for optical ph sensor","authors":"Noorlaila Ahmad, L. Heng, S. A. Hanifah, F. Salam","doi":"10.17576/MJAS-2019-2305-05","DOIUrl":"https://doi.org/10.17576/MJAS-2019-2305-05","url":null,"abstract":"","PeriodicalId":22844,"journal":{"name":"The Malaysian Journal of Analytical Sciences","volume":"150 1","pages":"799-811"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74714353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.17576/MJAS-2019-2304-20
Jacqueline Chong Kar Xin, Sharina Abu Hanifah, Norashikin Salleh, Normazida Rozi, Norfaizan Padli
{"title":"Analisis kandungan kepekatan logam berat dalam air balast dan air laut di pelabuhan tanjung pelepas","authors":"Jacqueline Chong Kar Xin, Sharina Abu Hanifah, Norashikin Salleh, Normazida Rozi, Norfaizan Padli","doi":"10.17576/MJAS-2019-2304-20","DOIUrl":"https://doi.org/10.17576/MJAS-2019-2304-20","url":null,"abstract":"","PeriodicalId":22844,"journal":{"name":"The Malaysian Journal of Analytical Sciences","volume":"110 1","pages":"736-747"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72673889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.17576/MJAS-2019-2304-21
Andi Rifki Rosandy, Y. Ying, Cheah Yoke Kqueen, Muntaz Abu Bakar, Azira Muhamad, R. Khalid
{"title":"Aktinobakteria psikrotoleran Barrientosiimonas humi 39T sebagai sumber sebatian diketopiperazin","authors":"Andi Rifki Rosandy, Y. Ying, Cheah Yoke Kqueen, Muntaz Abu Bakar, Azira Muhamad, R. Khalid","doi":"10.17576/MJAS-2019-2304-21","DOIUrl":"https://doi.org/10.17576/MJAS-2019-2304-21","url":null,"abstract":"","PeriodicalId":22844,"journal":{"name":"The Malaysian Journal of Analytical Sciences","volume":"9 1","pages":"748-762"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82437205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.17576/MJAS-2019-2304-16
Nurul Izzaty Hassan, Douglas Philp
{"title":"Sintesis dan sifat pengikatan makrosiklik tetrabutil-amida bagi penghasilan pseudorotazen","authors":"Nurul Izzaty Hassan, Douglas Philp","doi":"10.17576/MJAS-2019-2304-16","DOIUrl":"https://doi.org/10.17576/MJAS-2019-2304-16","url":null,"abstract":"","PeriodicalId":22844,"journal":{"name":"The Malaysian Journal of Analytical Sciences","volume":"2 1","pages":"690-702"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89786532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.17576/MJAS-2019-2304-14
A. R. Rosandy, Anis Asmi Azman, R. Khalid, Rizafizah Othaman, Mohamad Azwani Shah Mat Lazim, Iqbal M. Choudary, Yana M. Syah, Jalifah Latip, Ikram M. Said, Muntaz Abu Bakar
{"title":"Pemencilan komponen rotundona terbaharu daripada akar croton hirtus (euphorbiaceae)","authors":"A. R. Rosandy, Anis Asmi Azman, R. Khalid, Rizafizah Othaman, Mohamad Azwani Shah Mat Lazim, Iqbal M. Choudary, Yana M. Syah, Jalifah Latip, Ikram M. Said, Muntaz Abu Bakar","doi":"10.17576/MJAS-2019-2304-14","DOIUrl":"https://doi.org/10.17576/MJAS-2019-2304-14","url":null,"abstract":"","PeriodicalId":22844,"journal":{"name":"The Malaysian Journal of Analytical Sciences","volume":"31 1","pages":"677-681"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83905169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Solid polymer electrolyte (SPE) films were developed from poly(L-lactic acid)-poly(propylene glycol) (PLLA-PPG) with ethylene carbonate (EC) or propylene carbonate (PC) as plasticizer and lithium perchlorate (LiClO4) salt using solution casting method. The conductivity behaviours of the samples prepared were studied by electron impedance spectroscopy (EIS). From the EIS analysis, PLLA-PPG with 30 wt.% of EC and 25 wt.% of LiClO4 shows the highest conductivity value of 4.57 × 10 -5 S cm at room temperature. The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) confirm that there is chemical interaction between polymer host and lithium cation from lithium perchlorate. The shift of wavenumber for carbonyl (C=O) and ether (C-O-C) can be seen in ATR-FTIR spectrum. The crystallinity of PLLA was studied using X-ray diffraction (XRD) analysis. The absence of diffraction peaks corresponding to LiClO4 in SPE, which indicates that LiClO4 solvates well in the PLLA-PPG film. Thermal study by TGA indicated that PLLA-PPG SPE was thermally stable up to 270 oC.
{"title":"Kesan pemplastik dan litium perklorat terhadap elektrolit polimer pepejal poli(L-asid laktik)-poli(propilena glikol)","authors":"Siti Munirah Manap, Azizan Ahmad, Mohd Sani Sarjadi, Farah Hannan Anuar","doi":"10.17576/MJAS-2019-2304-17","DOIUrl":"https://doi.org/10.17576/MJAS-2019-2304-17","url":null,"abstract":"Solid polymer electrolyte (SPE) films were developed from poly(L-lactic acid)-poly(propylene glycol) (PLLA-PPG) with ethylene carbonate (EC) or propylene carbonate (PC) as plasticizer and lithium perchlorate (LiClO4) salt using solution casting method. The conductivity behaviours of the samples prepared were studied by electron impedance spectroscopy (EIS). From the EIS analysis, PLLA-PPG with 30 wt.% of EC and 25 wt.% of LiClO4 shows the highest conductivity value of 4.57 × 10 -5 S cm at room temperature. The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) confirm that there is chemical interaction between polymer host and lithium cation from lithium perchlorate. The shift of wavenumber for carbonyl (C=O) and ether (C-O-C) can be seen in ATR-FTIR spectrum. The crystallinity of PLLA was studied using X-ray diffraction (XRD) analysis. The absence of diffraction peaks corresponding to LiClO4 in SPE, which indicates that LiClO4 solvates well in the PLLA-PPG film. Thermal study by TGA indicated that PLLA-PPG SPE was thermally stable up to 270 oC.","PeriodicalId":22844,"journal":{"name":"The Malaysian Journal of Analytical Sciences","volume":"23 1","pages":"703-714"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86149688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.17576/MJAS-2019-2304-18
Siti Aishah Mohd Ali, Satirah Zainalabidin, Jalifah Latip
Phenolics content of two roselle varieties, H. sabdariffa var. UKMR-2 and H. sabdariffa var. UMKL-1 was determined based on high performance liquid chromatography method using a photodiode array detector (HPLC-PDA) with gradient elution. Roselle calyces were extracted with water via sonication (50 C, 30 minutes). The chromatographic separation was carried out on Purospher STAR RP-18e LichroCART column (250 mm × 4.6 mm × 5 μm) and detection was carried out at three different wavelengths (265, 320 and 520 nm). The developed HPLC method provided high percentage of recovery (95 102%), good linearity at R > 0.99, low limit of detection (LOD) and low limit of quantitation (LOQ). Two predominant anthocyanins were detected by HPLC-PDA in both H. sabdariffa varieties and were identified as delphinidin-3-O-sambubioside and cyanidin-3-Osambubioside. In addition, chlorogenic acid, caffeic acid and ascorbic acid were also detected. High content of phenolics and ascorbic acid were detected in UKMR-2 as compared to UMKL-1, suggested this variety have high potential for commercial cultivation.
采用光电二极管阵列检测器(HPLC-PDA) -梯度洗脱的高效液相色谱法测定了2个玫瑰茄品种H. sabdariffa var. UKMR-2和H. sabdariffa var. UMKL-1的酚类物质含量。水超声提取玫瑰花萼(50℃，30分钟)。色谱柱为Purospher STAR RP-18e LichroCART (250 mm × 4.6 mm × 5 μm)，检测波长为265、320和520 nm。该方法回收率高(95 102%)，线性关系好(R > 0.99)，具有低检出限和低定量限。用高效液相色谱- pda测定了两种主要的花青素，分别为delphinidin-3- o -sambubio苷和cyanidin-3- osambubio苷。此外，还检测出绿原酸、咖啡酸和抗坏血酸。与UMKL-1相比，UKMR-2中酚类物质和抗坏血酸含量较高，表明该品种具有较高的商业化栽培潜力。
{"title":"Analisis kuantitatif bagi sebatian fenolik dalam dua varieti roselle (Hibiscus sabdariffa) menggunakan kromatografi cecair berprestasi tinggi","authors":"Siti Aishah Mohd Ali, Satirah Zainalabidin, Jalifah Latip","doi":"10.17576/MJAS-2019-2304-18","DOIUrl":"https://doi.org/10.17576/MJAS-2019-2304-18","url":null,"abstract":"Phenolics content of two roselle varieties, H. sabdariffa var. UKMR-2 and H. sabdariffa var. UMKL-1 was determined based on high performance liquid chromatography method using a photodiode array detector (HPLC-PDA) with gradient elution. Roselle calyces were extracted with water via sonication (50 C, 30 minutes). The chromatographic separation was carried out on Purospher STAR RP-18e LichroCART column (250 mm × 4.6 mm × 5 μm) and detection was carried out at three different wavelengths (265, 320 and 520 nm). The developed HPLC method provided high percentage of recovery (95 102%), good linearity at R > 0.99, low limit of detection (LOD) and low limit of quantitation (LOQ). Two predominant anthocyanins were detected by HPLC-PDA in both H. sabdariffa varieties and were identified as delphinidin-3-O-sambubioside and cyanidin-3-Osambubioside. In addition, chlorogenic acid, caffeic acid and ascorbic acid were also detected. High content of phenolics and ascorbic acid were detected in UKMR-2 as compared to UMKL-1, suggested this variety have high potential for commercial cultivation.","PeriodicalId":22844,"journal":{"name":"The Malaysian Journal of Analytical Sciences","volume":"16 1","pages":"715-724"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84349614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-01DOI: 10.17576/mjas-2018-2206-15
Fatin Afifah Ahmad Kuthi, Nurulhuda Mohd Yunus, Goh Kae Horng, Khairiah Haji Badri
{"title":"Penentuan kandungan glukosa dalam Kenaf","authors":"Fatin Afifah Ahmad Kuthi, Nurulhuda Mohd Yunus, Goh Kae Horng, Khairiah Haji Badri","doi":"10.17576/mjas-2018-2206-15","DOIUrl":"https://doi.org/10.17576/mjas-2018-2206-15","url":null,"abstract":"","PeriodicalId":22844,"journal":{"name":"The Malaysian Journal of Analytical Sciences","volume":"10 1","pages":"1048-1056"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87347311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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