蜂蜜蒸发红火果皮提取物的抗菌活性研究

Dimas Wahyu Pradana, A. Manab, M. Sawitri
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引用次数: 0

摘要

合成酸奶含有益生菌和益生元。红火果果皮含有10.79%的果胶,可作为益生元。红龙果皮合成酸奶回味不佳。蜂蜜可以增强龙果皮合成酸奶的功能特性。添加益生元的酸奶可以提高益生菌酸奶的功能特性,提高消化道细菌的活力。本研究旨在测定蒸发红龙果皮提取物与蜂蜜合成酸奶的抑菌活性。这项研究是在布拉维贾亚大学畜牧学院动物产品技术实验室进行的。研究材料为体重在150 ~ 180 g之间的3月龄雄性褐家鼠15只,分为益生菌酸奶、添加红龙果皮提取物(Hylocereus polyrhizus) 20%(由10%脱脂乳和含有保加利亚乳杆菌和嗜热链球菌的酸奶发酵剂(1:1)、添加2%蜂蜜的合成酸奶3组。分别饲喂益生菌酸奶(P0)、20%红龙果皮提取物合成酸奶(P1)和20%红龙果皮提取物加2%蜂蜜甜味剂合成酸奶(P2)。试验期为30 d。然后解剖大鼠,取回肠粪便进行检测。所得资料采用描述性定性分析。结果表明,P0能减少大肠菌群和肠球菌的数量,但能增加总细菌、霉菌、酵母和大肠杆菌的数量。在合成酸奶(P1)中添加蒸发红龙果皮提取物,减少了细菌总数、大肠菌群和肠球菌,增加了霉菌、酵母和大肠杆菌。蜂蜜(P2)的添加降低了霉菌、酵母和肠球菌的总菌落,但增加了大肠杆菌。红龙果皮提取物合成酸奶中添加蜂蜜对酸奶中细菌总数、霉菌和酵母菌总数、大肠菌群总数、大肠杆菌总数和肠球菌总数均有影响。
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Antibacterial Activity of Synbiotic Yoghurt Peel Extract of Red Dragon Fruit (Hylocereus polyrhizus) Evaporation with Honey
Synbiotic yogurt contains both probiotics and prebiotics. The peel of red dragon fruit contains 10.79% pectin, which can be used as a prebiotic. Red dragon fruit peel synbiotic yoghurt has an unpleasant aftertaste. Honey can enhance the functional properties of dragon fruit peel synbiotic yogurt. Yogurt with the addition of prebiotics can increased the functional properties of probiotic yogurt and increase the viability of bacteria the digestive tract. The purpose of this study was to determine the antibacterial activity of synbiotic yogurt with evaporated red dragon fruit peel extract and honey. This research was conducted at the Laboratory of Animal Products Technology, Faculty of Animal Husbandry, Universitas Brawijaya. The research material is 3-month-old male 15 white rats (Rattus norvegicus) weighing between 150-180 g and divided into 3 groups, probiotic yoghurt, synbiotic yoghurt with addition of red dragon fruit peel extract (Hylocereus polyrhizus) 20% made from 10% skim milk and yoghurt starter containing Lactobacillus bulgaricus and Streptococcus thermophilus (1:1), and the addition of 2% honey to synbiotic. Animals were given probiotic yogurt (P0), 20% red dragon fruit peel extract synbiotic yogurt (P1), and 20% red dragon fruit peel extract synbiotic yogurt with 2% honey sweetener (P2). The duration of experimenting is 30 days. Afterward, white rats will be dissected and the faeces in the ileum will be taken for testing. The data obtained were analyzed using descriptive qualitative. The results showed that P0 did decrease coliform and Enterococcus spp but increase total bacteria, mold and yeast, and Escherichia coli. The addition of evaporated red dragon fruit peel extract in synbiotic yogurt (P1) did decrease total bacteria, coliform, and Enterococcus spp but increase in mold and yeast and Escherichia coli. The addition of honey (P2) did decrease total decrease, mold and yeast, and Enterococcus spp but increase Escherichia coli. The addition of honey to the synbiotic yogurt of red dragon fruit peel extract can affect the total number of bacteria, mold and yeast, coliform, Escherichia coli, and Enterococcus spp.
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