大豆叶和根对阿南布拉市市售腐坏蔬菜病原菌的植物化学和抑菌效果

Ejimofor, Chiamaka Frances, N. Enoch, O. Johnson, Afam-Ezeaku, Chikaodili Eziamaka, Mbaukwu, Onyinye Ann
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摘要

研究了豆叶和豆根对阿南布拉市市售腐坏蔬菜中病原菌的植物化学和抗真菌活性。通过将100g、100ml无菌蒸馏水和乙醇与每种植物部位混合,从每种植物成分中产生两(2)种不同浓度的提取物。使用几种常规技术对这些植物进行植物化学筛选,确定了所有植物中存在的生物碱、皂苷、单宁、类黄酮、植酸盐、草酸盐和苯酚,但含量不同。比较常用抗生素(氟康唑30 g/ml)与植物提取物的效果。经致病性试验,好菜腐病主要由黑曲霉、茄枯菌、青霉、根霉、毛霉等病原菌引起,以豆根乙醇提取物效果较好。提取溶剂、提取浓度、检测病原菌等因素均对提取物的药效有影响。随着提取物浓度的增加,真菌生长抑制量也增加。所有试验真菌的菌丝发育均受到豆根的有效/高度抑制,但水提取物的抑制程度较低。农民应该使用豆根乙醇提取物,而不是合成的或商业化的杀菌剂,因为它们对蔬菜储藏真菌有潜在的真菌毒性。
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The Phytochemical and Antifungal Efficiency of Bean Leaf and Root against Some Pathogenic Fungi Isolated from Spoilt Vegetables Sold within Anambra Metropolies
Investigated in vitro were the phytochemical and antifungal properties of bean leaf and root against certain pathogenic fungi isolated from spoiled vegetables marketed in Anambra metropolises. By combining 100g, 100ml of sterile distilled water, and ethanol with each plant part, two (2) distinct extract concentrations were produced from each plant component. The plants were subjected to phytochemical screening using several conventional techniques, which identified the presence of alkaloids, saponins, tannins, flavonoids, phytate, oxalate, and phenol in all of the plants but at various amounts. The effects of common antibiotics (Fluconazole 30 g/ml) were compared to those of plant extracts. According to a pathogenicity test, good vegetables rot was caused by Aspergillus niger, Fusarium solani, Penicillium sp, Rhizopus sp, and Mucor spp. The ethanol extract from beans root turned out to be more effective. The extraction solvent, extract concentration, and test pathogens all had an impact on the extract's effectiveness. When extract concentration rose, the amount of fungal growth inhibition also rose. All of the test fungi's mycelia development was effectively/highly inhibited by beans root, although water extracts exhibited a lower degree of inhibition. Farmers should utilise bean root ethanolic extracts instead of synthetic or commercial fungicides due to their fungitoxic potential against vegetable storage fungi.
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