卵母细胞和山羊卵泡输卵管表达特异性糖蛋白1 (OVGP1)的研究

A. Firmawati, Herawati, H. Pratiwi, L. ‘Ainiyah, A. S. R. A. Rozzaaq
{"title":"卵母细胞和山羊卵泡输卵管表达特异性糖蛋白1 (OVGP1)的研究","authors":"A. Firmawati, Herawati, H. Pratiwi, L. ‘Ainiyah, A. S. R. A. Rozzaaq","doi":"10.5220/0009586500490052","DOIUrl":null,"url":null,"abstract":": Oviduct Specific Glycoprotein (OVGP1) is a glycoprotein that has been identified as a protein secreted from unciliated secretory epithelial cells in oviducts with a molecular weight of 65 kDa in goats. The expression of this protein is very dependent on estrogen levels and the oestrus phase of the species. On the other hand, glycoprotein plays an important role in the process of oocyte maturation, spermatozoa capitation, and early embryonic development. The purpose of this research was to find the location of OVGP1 expression in oocytes and follicles of kacang goat (Capra hircus), an endogenous goat from Indonesian . The methods used in this study are: immunocytochemical techniques to see OVGP1 expression on oocytes and on goat follicles. OVGP1 expression in goat oocytes (Capra hircus) observed using immunocytochemical techniques was detected in the cumulus ooporus section, the zona pellucida, perivitelline space, and plasma oocyte membrane, whereas in the follicle the OVGP1 expression observed using immunohistochemical methods showed that OVGP1 was expressed in granulosa cells, external and internal theca cells.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":"19 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Study of Oviduct Expression Specificct Glycoprotein1 (OVGP1) on Oocyte and Goat Follicles (Capra hircus)\",\"authors\":\"A. Firmawati, Herawati, H. Pratiwi, L. ‘Ainiyah, A. S. R. A. Rozzaaq\",\"doi\":\"10.5220/0009586500490052\",\"DOIUrl\":null,\"url\":null,\"abstract\":\": Oviduct Specific Glycoprotein (OVGP1) is a glycoprotein that has been identified as a protein secreted from unciliated secretory epithelial cells in oviducts with a molecular weight of 65 kDa in goats. The expression of this protein is very dependent on estrogen levels and the oestrus phase of the species. On the other hand, glycoprotein plays an important role in the process of oocyte maturation, spermatozoa capitation, and early embryonic development. The purpose of this research was to find the location of OVGP1 expression in oocytes and follicles of kacang goat (Capra hircus), an endogenous goat from Indonesian . The methods used in this study are: immunocytochemical techniques to see OVGP1 expression on oocytes and on goat follicles. OVGP1 expression in goat oocytes (Capra hircus) observed using immunocytochemical techniques was detected in the cumulus ooporus section, the zona pellucida, perivitelline space, and plasma oocyte membrane, whereas in the follicle the OVGP1 expression observed using immunohistochemical methods showed that OVGP1 was expressed in granulosa cells, external and internal theca cells.\",\"PeriodicalId\":20550,\"journal\":{\"name\":\"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering\",\"volume\":\"19 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5220/0009586500490052\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5220/0009586500490052","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

输卵管特异性糖蛋白(OVGP1)是一种糖蛋白,由山羊输卵管内的非纤毛分泌上皮细胞分泌,分子量为65 kDa。这种蛋白的表达非常依赖于雌激素水平和物种的发情期。另一方面,糖蛋白在卵母细胞成熟、精子头化和早期胚胎发育过程中起着重要作用。本研究的目的是寻找OVGP1在印尼内源山羊卡仓山羊(Capra hircus)卵母细胞和卵泡中的表达位置。本研究使用的方法是:免疫细胞化学技术观察OVGP1在卵母细胞和山羊卵泡中的表达。在山羊卵母细胞(Capra hircus)中,OVGP1在卵孔积云切片、透明带、卵泡周围间隙和卵浆膜中表达,而在卵泡中,OVGP1在颗粒细胞、外膜细胞和内膜细胞中表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Study of Oviduct Expression Specificct Glycoprotein1 (OVGP1) on Oocyte and Goat Follicles (Capra hircus)
: Oviduct Specific Glycoprotein (OVGP1) is a glycoprotein that has been identified as a protein secreted from unciliated secretory epithelial cells in oviducts with a molecular weight of 65 kDa in goats. The expression of this protein is very dependent on estrogen levels and the oestrus phase of the species. On the other hand, glycoprotein plays an important role in the process of oocyte maturation, spermatozoa capitation, and early embryonic development. The purpose of this research was to find the location of OVGP1 expression in oocytes and follicles of kacang goat (Capra hircus), an endogenous goat from Indonesian . The methods used in this study are: immunocytochemical techniques to see OVGP1 expression on oocytes and on goat follicles. OVGP1 expression in goat oocytes (Capra hircus) observed using immunocytochemical techniques was detected in the cumulus ooporus section, the zona pellucida, perivitelline space, and plasma oocyte membrane, whereas in the follicle the OVGP1 expression observed using immunohistochemical methods showed that OVGP1 was expressed in granulosa cells, external and internal theca cells.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
In Silico Prediction of High Potential Jararhagin Inhibitor: Comparison of Batimastat, EDTA and Hydroxytyrosol Sumbawa Horse Milk as a Prevention of Inflammatory Bowel Disease (IBD) in Animal Model based on IL-6 Expression and Gastric Histopathology The Differences of Electrophoretic Profile and Snake Venom Phospholipase A2 (svPLA2) Activity from the Venom of Javan Spitting Cobra, Naja sputatrix, based on Body Scales Color and Storage Condition Helminthiasis and Aspergillosis Suspect Examination in Pigeon Storage Duration Effect of Kelor Leaf (Moringa oleifera) Extracts with Methanol against Growth of Streptococcus agalactiae and Escherichia coli Caused Mastitis in Dairy Cattle
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1