Pub Date : 2019-01-01DOI: 10.5220/0009586001170121
A. Haskito, A. Setianingrum, F. Dameanti, M. Fatmawati
Yoghurt is a functional food with various health benefit. Goat milk yoghurt contains bioactive peptides which have antioxidant potential. Rice bran is known for its good nutrition and high fiber and rich in bioactive components as potential antioxidant. White rice bran flour fortification into yoghurt processed from goat's milk is expected to increase the yoghurt’s nutritional value, especially in fiber and antioxidants. The aim of this research was to create yoghurt from goat’s milk fortified by white rice bran flour and to analyze said yogurt with hedonic test and hedonic quality test for organoleptic properties namely sour taste, goat aroma, bran aroma, and consistency. This research’s methodology was an experimental study which used a Completely Randomized Design (CRD) method for one treatment with five repetitions. The treatment factor is fortificationby 4% white rice bran flour in pasteurized goat milk which has been added with starter of Lactobacillus bulgaricus, Streptococcus thermophilus, and Lactobacillus acidophilus 3% during incubational together until the milk transformed into yoghurt. The hedonic test and hedonic quality tests involved 30 panelists. The data is analysed by calculating average values. The results showed that panelists enjoyed overall characteristics such as sour taste, goat aroma, bran aroma, and yoghurt’s consistency. The panelists stated that fortified goat milk yogurt had sour taste, no goat meat and bran aroma, with adequately solid consistency.
{"title":"Organoleptic Properties Evaluation of Goat Milk Yogurt with White Rice Bran Flour Fortification","authors":"A. Haskito, A. Setianingrum, F. Dameanti, M. Fatmawati","doi":"10.5220/0009586001170121","DOIUrl":"https://doi.org/10.5220/0009586001170121","url":null,"abstract":"Yoghurt is a functional food with various health benefit. Goat milk yoghurt contains bioactive peptides which have antioxidant potential. Rice bran is known for its good nutrition and high fiber and rich in bioactive components as potential antioxidant. White rice bran flour fortification into yoghurt processed from goat's milk is expected to increase the yoghurt’s nutritional value, especially in fiber and antioxidants. The aim of this research was to create yoghurt from goat’s milk fortified by white rice bran flour and to analyze said yogurt with hedonic test and hedonic quality test for organoleptic properties namely sour taste, goat aroma, bran aroma, and consistency. This research’s methodology was an experimental study which used a Completely Randomized Design (CRD) method for one treatment with five repetitions. The treatment factor is fortificationby 4% white rice bran flour in pasteurized goat milk which has been added with starter of Lactobacillus bulgaricus, Streptococcus thermophilus, and Lactobacillus acidophilus 3% during incubational together until the milk transformed into yoghurt. The hedonic test and hedonic quality tests involved 30 panelists. The data is analysed by calculating average values. The results showed that panelists enjoyed overall characteristics such as sour taste, goat aroma, bran aroma, and yoghurt’s consistency. The panelists stated that fortified goat milk yogurt had sour taste, no goat meat and bran aroma, with adequately solid consistency.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86197099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009587701410146
N. Lolok, Nurlena Ikawati, F. Siharis
The issue of Indonesian population density is one of must be faced problems. Stem bark of mangrove Avicennia marina is one of many plants can be used as contraception. Recently reported, in many countries this Avicennia marina used as antifertility nevertheless there are no focus research of the topic proven. The object of this study is to determine the effect of stem bark extract of mangrove Avicennia marina on number, motility and viability of spermatozoa of male white rats and also to assess the effect of certain treatment group of some fractions compared to control CMC-Na. This study is experimentally designed in laboratorium. Stem bark of Avicennia marina was extracted by soxhlet extraction with methanol solvent, while animal testing were classified to 4 group (each group for 6 rats randomly). The subject were treated along 34 days everyday, the group 1: 1% CMC-Na as a control; Group 2: low dose methanol suspension extract of 1.25%; Group 3: medium dose methanol suspension extract of 2.5%; and Group 4: high dose methanol suspension extract of 5%.Motility test was conducted by putting a drop of sperm on improved neubauer counting chamber and observed by Olympus BX53F microscope (400x magnifying). Then the data analyzed by ANOVA (p value 95%) prior to post hoc test by Duncan (SPSS 20).Motility index of each group 5%, 2.5%, 1.25% and controlwere35%, 47%, 67% and 79% respectively. While, for viability index in group 5%, 2.5%,1.25% and control were 62%, 76%, 73% and 88% respectively.The treatment of stem bark extract of mangrove Avicennia marina in male white rats had significantly related to motility and viability index of male rats sperm (p <0.005). This results means that stem bark extract of mangrove Avicennia marina showed antifertility effect in male rats.
{"title":"The Potency of Antifertility Effect of Stem Bark Extract of Mangrove (Avicennia Marina) on Male White Rats (Rattus Novergicus)","authors":"N. Lolok, Nurlena Ikawati, F. Siharis","doi":"10.5220/0009587701410146","DOIUrl":"https://doi.org/10.5220/0009587701410146","url":null,"abstract":"The issue of Indonesian population density is one of must be faced problems. Stem bark of mangrove Avicennia marina is one of many plants can be used as contraception. Recently reported, in many countries this Avicennia marina used as antifertility nevertheless there are no focus research of the topic proven. The object of this study is to determine the effect of stem bark extract of mangrove Avicennia marina on number, motility and viability of spermatozoa of male white rats and also to assess the effect of certain treatment group of some fractions compared to control CMC-Na. This study is experimentally designed in laboratorium. Stem bark of Avicennia marina was extracted by soxhlet extraction with methanol solvent, while animal testing were classified to 4 group (each group for 6 rats randomly). The subject were treated along 34 days everyday, the group 1: 1% CMC-Na as a control; Group 2: low dose methanol suspension extract of 1.25%; Group 3: medium dose methanol suspension extract of 2.5%; and Group 4: high dose methanol suspension extract of 5%.Motility test was conducted by putting a drop of sperm on improved neubauer counting chamber and observed by Olympus BX53F microscope (400x magnifying). Then the data analyzed by ANOVA (p value 95%) prior to post hoc test by Duncan (SPSS 20).Motility index of each group 5%, 2.5%, 1.25% and controlwere35%, 47%, 67% and 79% respectively. While, for viability index in group 5%, 2.5%,1.25% and control were 62%, 76%, 73% and 88% respectively.The treatment of stem bark extract of mangrove Avicennia marina in male white rats had significantly related to motility and viability index of male rats sperm (p <0.005). This results means that stem bark extract of mangrove Avicennia marina showed antifertility effect in male rats.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85869477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009587200580061
H. Pratiwi, Analis Wisnu Wardana, A. P. D. A. Okatvianie, A. Haryo, N. Fauziah, Rizky Pamwidya Abqariyyan, Rifkytri Aditia, Syadza Afra
: The zygote develops into an embryo after going through the process of organogenesis. During organogenesis, there is also the process of angiogenesis, the formation of blood vessels. However, the process of angiogenesis can be hampered by several factors including the presence of foreign compounds that enter the parent body. One compound that is believed to have an angiogenesis inhibiting effect is curcumin from the turmeric rhizome simplisia. Inhibition of angiogenesis due to curcumin induction can have an impact on embryonic organogenesis in terms of embryonic body weigth and thicness so this study was conducted to determine the teratogenic effects of curcumin of turmeric rhizome simplisia on chicken embryos. This study used fifteen unincubated chicken eggs divided into three groups, the negative control group, the group injected with turmeric rhizome simplisia at a dose of 0.48 mg/gr and DMSO 2% as much as 0.25 mL, and the group injected with turmeric rhizome simplisia at a dose of 0.96 mg/gr and DMSO 2% as much as 0.25 mL. All groups were incubated for 10 days at 38 °C. The parameters measured were embryo body weight use digital scales and measurement of the body thicness with calipers. The results showed that the turmeric rhizome simplisia at dose of 0,48,mg/gr cause died in one of incubated chicken egg and at dose of 0.96 mg/gr cause two of five incubated chicken egg was died. The left eggs from group of dose 0.48 mg/gr and 0.98 mg/gr have decreased of body weight and thicness compared with negative control group. It was showed that turmeric rhizome simplicial in this doses have effect in decrease body weight and thickness of chicken embryos.
{"title":"The Body Weight and Thickness in Chicken Embryo Incubated for 10 Days Induced by Turmeric Rhizome Simplisia","authors":"H. Pratiwi, Analis Wisnu Wardana, A. P. D. A. Okatvianie, A. Haryo, N. Fauziah, Rizky Pamwidya Abqariyyan, Rifkytri Aditia, Syadza Afra","doi":"10.5220/0009587200580061","DOIUrl":"https://doi.org/10.5220/0009587200580061","url":null,"abstract":": The zygote develops into an embryo after going through the process of organogenesis. During organogenesis, there is also the process of angiogenesis, the formation of blood vessels. However, the process of angiogenesis can be hampered by several factors including the presence of foreign compounds that enter the parent body. One compound that is believed to have an angiogenesis inhibiting effect is curcumin from the turmeric rhizome simplisia. Inhibition of angiogenesis due to curcumin induction can have an impact on embryonic organogenesis in terms of embryonic body weigth and thicness so this study was conducted to determine the teratogenic effects of curcumin of turmeric rhizome simplisia on chicken embryos. This study used fifteen unincubated chicken eggs divided into three groups, the negative control group, the group injected with turmeric rhizome simplisia at a dose of 0.48 mg/gr and DMSO 2% as much as 0.25 mL, and the group injected with turmeric rhizome simplisia at a dose of 0.96 mg/gr and DMSO 2% as much as 0.25 mL. All groups were incubated for 10 days at 38 °C. The parameters measured were embryo body weight use digital scales and measurement of the body thicness with calipers. The results showed that the turmeric rhizome simplisia at dose of 0,48,mg/gr cause died in one of incubated chicken egg and at dose of 0.96 mg/gr cause two of five incubated chicken egg was died. The left eggs from group of dose 0.48 mg/gr and 0.98 mg/gr have decreased of body weight and thicness compared with negative control group. It was showed that turmeric rhizome simplicial in this doses have effect in decrease body weight and thickness of chicken embryos.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88322903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009587501010104
M. Fatmawati, D. K. Mayashinta, M. Padaga, A. Setianingrum, Fidi Nur Aini
Collaboration research is interdisciplinary effort that seek optimal health for people and animal. Toxoplamosia is zoonotic disease that caused by Toxoplasma gondii. It’s intracelluer protozoan infection that could be found in meat or milk from infected animal. The epidemiology, prevention and control strategies are reviewed, with the objective of integrative reseach. To reduce the disease in human, intervention are needed in the animal reservoir, necessitating close collaboration between booth the human and veterinary medical sectors. This study showed that toxoplamosis in human are positive that infected 3 farmers (from 14 farmer). Prevalence toxopalmosis in goat are between 22% 100%. A high prevalence on one farm allows for human infected with toxoplamosis.
{"title":"Colaboration Reseach of Seroprevalensi Toxoplamosis in Goat and Animal","authors":"M. Fatmawati, D. K. Mayashinta, M. Padaga, A. Setianingrum, Fidi Nur Aini","doi":"10.5220/0009587501010104","DOIUrl":"https://doi.org/10.5220/0009587501010104","url":null,"abstract":"Collaboration research is interdisciplinary effort that seek optimal health for people and animal. Toxoplamosia is zoonotic disease that caused by Toxoplasma gondii. It’s intracelluer protozoan infection that could be found in meat or milk from infected animal. The epidemiology, prevention and control strategies are reviewed, with the objective of integrative reseach. To reduce the disease in human, intervention are needed in the animal reservoir, necessitating close collaboration between booth the human and veterinary medical sectors. This study showed that toxoplamosis in human are positive that infected 3 farmers (from 14 farmer). Prevalence toxopalmosis in goat are between 22% 100%. A high prevalence on one farm allows for human infected with toxoplamosis.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89994602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009588100790083
U. Yanuhar, M. Musa, D. Arfiati, N. Caesar, N. S. Junirahma
Grouper (Cromileptes altivelis) is a species of fish with important economic values both in the national and international markets. The disease that has been reported by researchers is Viral Nervous Necrosis (VNN) which can cause mass death in groupers, especially in larval and juvenile stadia. Based on the problems, a research is needed on haematological analysis of groupers (Cromileptes altivelis) infected with Viral Nervous Necrosis by in-vivo testing using protein fragments of C. vulgaris. This research employed an experiment method using 5 treatments, namely (A) healthy fish, (B) VNN-infected fish, (C) VNN-infected fish with administration of C.vulgaris crude extracts of 17 μg mL-1, (D) VNN-infected fish with administration of C.vulgaris crude extract of 33 μg mL-1, and (E) VNN-infected fish with administration of C.vulgaris crude extract of 50 μg mL-1. Observations of haematological parameters included erythrocytes, leukocytes, haemoglobin, and haematocrit. The observation results showed an erythrocyte value of 97 x 104 cells/mm3 in treatment (C), 107 x 104 cells/mm3 in treatment (D), and 94 x 104 cells/mm3 in treatment (E). The observation results of leukocyte values were 150,000 cells/mm3 in treatment (C), 133,300 cells/mm3 in treatment (D), and 139,000 cells/mm3 in treatment (E). Furthermore, the observation results of haemoglobin showed a value of 5 gr/100 ml in treatment (C), 6 gr/100 ml in treatment (D), and 5 gr/100 ml in treatment (E). As for the haematocrit parameter, the results obtained from the observation were 18% in treatment (C), 22% in treatment (D), and 15% in treatment (E). Based on this research, the haematological status of VNNinfected groupers was not good. However, the results of the in-vivo testing conducted showed that administration of C. vulgaris extract gave a positive result on improving the haematological status of groupers (C. altivelis) infected with VNN with the optimal dose of 33 μg mL-1.
{"title":"In-vivo Test of Chlorella Protein Fragments as Nucleotide Vaccine Candidates in Grouper Viral Nervous Necrosis (VNN) Infection against Haematological Response","authors":"U. Yanuhar, M. Musa, D. Arfiati, N. Caesar, N. S. Junirahma","doi":"10.5220/0009588100790083","DOIUrl":"https://doi.org/10.5220/0009588100790083","url":null,"abstract":"Grouper (Cromileptes altivelis) is a species of fish with important economic values both in the national and international markets. The disease that has been reported by researchers is Viral Nervous Necrosis (VNN) which can cause mass death in groupers, especially in larval and juvenile stadia. Based on the problems, a research is needed on haematological analysis of groupers (Cromileptes altivelis) infected with Viral Nervous Necrosis by in-vivo testing using protein fragments of C. vulgaris. This research employed an experiment method using 5 treatments, namely (A) healthy fish, (B) VNN-infected fish, (C) VNN-infected fish with administration of C.vulgaris crude extracts of 17 μg mL-1, (D) VNN-infected fish with administration of C.vulgaris crude extract of 33 μg mL-1, and (E) VNN-infected fish with administration of C.vulgaris crude extract of 50 μg mL-1. Observations of haematological parameters included erythrocytes, leukocytes, haemoglobin, and haematocrit. The observation results showed an erythrocyte value of 97 x 104 cells/mm3 in treatment (C), 107 x 104 cells/mm3 in treatment (D), and 94 x 104 cells/mm3 in treatment (E). The observation results of leukocyte values were 150,000 cells/mm3 in treatment (C), 133,300 cells/mm3 in treatment (D), and 139,000 cells/mm3 in treatment (E). Furthermore, the observation results of haemoglobin showed a value of 5 gr/100 ml in treatment (C), 6 gr/100 ml in treatment (D), and 5 gr/100 ml in treatment (E). As for the haematocrit parameter, the results obtained from the observation were 18% in treatment (C), 22% in treatment (D), and 15% in treatment (E). Based on this research, the haematological status of VNNinfected groupers was not good. However, the results of the in-vivo testing conducted showed that administration of C. vulgaris extract gave a positive result on improving the haematological status of groupers (C. altivelis) infected with VNN with the optimal dose of 33 μg mL-1.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80469371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009587901520156
P. Surjowardojo, Rachmad Dharmawan, .. Rifai, Ike Ambarwati
The research aimed to determine the storage duration effectiveness of Moringaoleiferaleaf extract to inhibit the growth of Streptococcus agalactiaeand Escherichia coli causemastitis on dairy cows. The materials were Streptococcus agalactiae and Escherichia coli from Bacteriology Laboratory Agriculture Faculty, Brawijaya University counted as 108 CFU/ml and Moringa oleifera leaf. This research method was an experiment Completely Randomized Design 5 treatments and 5 replications. Storage duration treatment was P0 (control), P1 (2nd day), P2 (4th day), P3 (6th day), P4 (8th day) on the same concentrations 70%. The variable measured was the diameter of the inhibition zone. The data analyzed using ANOVA followed by the Duncan test. The results showed that Moringa oleifera leaf extract had difference highly significant capability to inhibit the growth of Streptococcus agalactiae and Escherichia coli (P < 0.01). The Capability of Moringa leaf extract to maintain bacterial growth inhibition until day 2 for Streptococcus agalactiae and Escherichia coli. Maximum storage time until day 2 to maintain the effectiveness of Moringa leaf extract in inhibiting the growth of Streptococcus agalactiae and Escherichia coli.
{"title":"Storage Duration Effect of Kelor Leaf (Moringa oleifera) Extracts with Methanol against Growth of Streptococcus agalactiae and Escherichia coli Caused Mastitis in Dairy Cattle","authors":"P. Surjowardojo, Rachmad Dharmawan, .. Rifai, Ike Ambarwati","doi":"10.5220/0009587901520156","DOIUrl":"https://doi.org/10.5220/0009587901520156","url":null,"abstract":"The research aimed to determine the storage duration effectiveness of Moringaoleiferaleaf extract to inhibit the growth of Streptococcus agalactiaeand Escherichia coli causemastitis on dairy cows. The materials were Streptococcus agalactiae and Escherichia coli from Bacteriology Laboratory Agriculture Faculty, Brawijaya University counted as 108 CFU/ml and Moringa oleifera leaf. This research method was an experiment Completely Randomized Design 5 treatments and 5 replications. Storage duration treatment was P0 (control), P1 (2nd day), P2 (4th day), P3 (6th day), P4 (8th day) on the same concentrations 70%. The variable measured was the diameter of the inhibition zone. The data analyzed using ANOVA followed by the Duncan test. The results showed that Moringa oleifera leaf extract had difference highly significant capability to inhibit the growth of Streptococcus agalactiae and Escherichia coli (P < 0.01). The Capability of Moringa leaf extract to maintain bacterial growth inhibition until day 2 for Streptococcus agalactiae and Escherichia coli. Maximum storage time until day 2 to maintain the effectiveness of Moringa leaf extract in inhibiting the growth of Streptococcus agalactiae and Escherichia coli.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75779208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009586500490052
A. Firmawati, Herawati, H. Pratiwi, L. ‘Ainiyah, A. S. R. A. Rozzaaq
: Oviduct Specific Glycoprotein (OVGP1) is a glycoprotein that has been identified as a protein secreted from unciliated secretory epithelial cells in oviducts with a molecular weight of 65 kDa in goats. The expression of this protein is very dependent on estrogen levels and the oestrus phase of the species. On the other hand, glycoprotein plays an important role in the process of oocyte maturation, spermatozoa capitation, and early embryonic development. The purpose of this research was to find the location of OVGP1 expression in oocytes and follicles of kacang goat (Capra hircus), an endogenous goat from Indonesian . The methods used in this study are: immunocytochemical techniques to see OVGP1 expression on oocytes and on goat follicles. OVGP1 expression in goat oocytes (Capra hircus) observed using immunocytochemical techniques was detected in the cumulus ooporus section, the zona pellucida, perivitelline space, and plasma oocyte membrane, whereas in the follicle the OVGP1 expression observed using immunohistochemical methods showed that OVGP1 was expressed in granulosa cells, external and internal theca cells.
{"title":"Study of Oviduct Expression Specificct Glycoprotein1 (OVGP1) on Oocyte and Goat Follicles (Capra hircus)","authors":"A. Firmawati, Herawati, H. Pratiwi, L. ‘Ainiyah, A. S. R. A. Rozzaaq","doi":"10.5220/0009586500490052","DOIUrl":"https://doi.org/10.5220/0009586500490052","url":null,"abstract":": Oviduct Specific Glycoprotein (OVGP1) is a glycoprotein that has been identified as a protein secreted from unciliated secretory epithelial cells in oviducts with a molecular weight of 65 kDa in goats. The expression of this protein is very dependent on estrogen levels and the oestrus phase of the species. On the other hand, glycoprotein plays an important role in the process of oocyte maturation, spermatozoa capitation, and early embryonic development. The purpose of this research was to find the location of OVGP1 expression in oocytes and follicles of kacang goat (Capra hircus), an endogenous goat from Indonesian . The methods used in this study are: immunocytochemical techniques to see OVGP1 expression on oocytes and on goat follicles. OVGP1 expression in goat oocytes (Capra hircus) observed using immunocytochemical techniques was detected in the cumulus ooporus section, the zona pellucida, perivitelline space, and plasma oocyte membrane, whereas in the follicle the OVGP1 expression observed using immunohistochemical methods showed that OVGP1 was expressed in granulosa cells, external and internal theca cells.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78553294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009586200330036
A. Haryo, R. Rahman
Pigeons are commonly maintained using a simple cage system, this system also easier to transmit disease such as helminthiasis and aspergillosis to infect pigeons. The air of this study was to know and diagnose changes in macroscopic and microscopic with histopathology method in pigeons. The organs examined are proventriculus, intestine, liver, and skin. Macroscopic changes were seen in liver change color into brownish-yellow on right lobes of the liver, hiperemi proventriculus, hemorrhage, and swelling intestine, baldness, and crust in the upper neck skin. Microscopic changes seen in hepar are white blood cell infiltration in triad portal, congesti and ulcer mucosa gland proventriculus, intestine shown epithelial erosion, rupture villi, hemorrhage and hyperplasia of cell goblet and skin shown black colored infiltration in dermis allegedly infected by Aspergillosis sp. From examination and observation, it can be concluded that macroscopic and microscopic changes lead to the helminthiasis and suspected Aspergillosis sp.
{"title":"Helminthiasis and Aspergillosis Suspect Examination in Pigeon","authors":"A. Haryo, R. Rahman","doi":"10.5220/0009586200330036","DOIUrl":"https://doi.org/10.5220/0009586200330036","url":null,"abstract":"Pigeons are commonly maintained using a simple cage system, this system also easier to transmit disease such as helminthiasis and aspergillosis to infect pigeons. The air of this study was to know and diagnose changes in macroscopic and microscopic with histopathology method in pigeons. The organs examined are proventriculus, intestine, liver, and skin. Macroscopic changes were seen in liver change color into brownish-yellow on right lobes of the liver, hiperemi proventriculus, hemorrhage, and swelling intestine, baldness, and crust in the upper neck skin. Microscopic changes seen in hepar are white blood cell infiltration in triad portal, congesti and ulcer mucosa gland proventriculus, intestine shown epithelial erosion, rupture villi, hemorrhage and hyperplasia of cell goblet and skin shown black colored infiltration in dermis allegedly infected by Aspergillosis sp. From examination and observation, it can be concluded that macroscopic and microscopic changes lead to the helminthiasis and suspected Aspergillosis sp.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75579703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009586901260132
D. E. Putri, A. Aulanni'am, A. Srihardyastuti
Cardiovascular disease is a disease that occurs due to a disturbance in the function of the heart and blood vessels such as coronary heart disease (PJK), hypertension and stroke. One cause of PJK is a condition of hypercholesterolemia. Hypercholesterolemia is a condition which cholesterol level more than 200 mg / dL. High cholesterol dietary can cause an increase in free radicals that cause oxidative stress. Fermented milk Lactobacillus casei thought to contain biopeptida as antioxsidant. This study was to determine the effect of fermented milk Lactobacillus casei in lowering levels of MDA and repairing protein profiles of heart. Animal that used is a rat (Rattus norvegicus, males, aged 2-3 months, weightabout 100-250 gram. Rats divided into normal rats, hypercholesterolemia rats , hypercholesterolemia rats and therapy 1 mL and 2 mL . High cholesterol dietary used egg yolk, cholesterol pure and cholic acid that give in forcefeeding for 4 weeks.Therapy of Lactobacillus casei fermented milk given for 2 weeks in. Level of MDA was measured using the method of TBA and cardiac protein bands was tested using SDS-PAGE. The result of Research obtained showed Lactobacillus casei fermented milk therapy was significantly (p <0.05) lower levels of MDA and affect cardiac protein bands of rats. Doses of 2 mL is the best dose with decreased levels of MDA by 43.11% and is able to restore protein band profiles such as Lactobacillus casei normal. In conclusion tha fermented milk can be used as an alternative treatment of hypercholesterolemia
{"title":"Therapy of Fermented Milk Lactobacillus Casei Strain Shirota to Level of Malondialdehyde (MDA) and Proteind Bands the Hearth the White Rats (Rattus Norvegicus) That given High Cholesterol Dietary","authors":"D. E. Putri, A. Aulanni'am, A. Srihardyastuti","doi":"10.5220/0009586901260132","DOIUrl":"https://doi.org/10.5220/0009586901260132","url":null,"abstract":"Cardiovascular disease is a disease that occurs due to a disturbance in the function of the heart and blood vessels such as coronary heart disease (PJK), hypertension and stroke. One cause of PJK is a condition of hypercholesterolemia. Hypercholesterolemia is a condition which cholesterol level more than 200 mg / dL. High cholesterol dietary can cause an increase in free radicals that cause oxidative stress. Fermented milk Lactobacillus casei thought to contain biopeptida as antioxsidant. This study was to determine the effect of fermented milk Lactobacillus casei in lowering levels of MDA and repairing protein profiles of heart. Animal that used is a rat (Rattus norvegicus, males, aged 2-3 months, weightabout 100-250 gram. Rats divided into normal rats, hypercholesterolemia rats , hypercholesterolemia rats and therapy 1 mL and 2 mL . High cholesterol dietary used egg yolk, cholesterol pure and cholic acid that give in forcefeeding for 4 weeks.Therapy of Lactobacillus casei fermented milk given for 2 weeks in. Level of MDA was measured using the method of TBA and cardiac protein bands was tested using SDS-PAGE. The result of Research obtained showed Lactobacillus casei fermented milk therapy was significantly (p <0.05) lower levels of MDA and affect cardiac protein bands of rats. Doses of 2 mL is the best dose with decreased levels of MDA by 43.11% and is able to restore protein band profiles such as Lactobacillus casei normal. In conclusion tha fermented milk can be used as an alternative treatment of hypercholesterolemia","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77303414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009586300370040
A. W. Wardhana, A. Firmawati, A. Haryo, Kevin Ersananda, Nabila Safira, Tiara Balqhis
Fetal development in domestic cat can be monitored using ultrasonography to control viability, maintain the nutrition care, and drug use. Due to control the viability of foetuses, this study observed the correlation of gestational sac diameter, fetal head diameter, and fetal heart diameter compared to gestational age. This study was performed by using 9 queens that divided into three groups. The first group measured the gestational sac diameter, the second group measured the fetal head diameter, and the third group measured the fetal heart diameter. Gestational sac diameter and fetal head diameter are measured by specific formula, while the fetal heart diameter calculated by measuring point to point of heart edge and compare to head diameter to verify the gestational age. As the result, the gestational sac diameter, the feal head diameter, and the fetal heart rate of domestic cat in Indonesia have correlation to gestational age and also can be used to estimate the gestational edge.
{"title":"Correlation of Gestational Sac Diameter, Fetal Heart Diameter, and Fetal Head Diameter to Gestational Age of Local Cats (Felis domesticus) Pregnancy in Indonesia","authors":"A. W. Wardhana, A. Firmawati, A. Haryo, Kevin Ersananda, Nabila Safira, Tiara Balqhis","doi":"10.5220/0009586300370040","DOIUrl":"https://doi.org/10.5220/0009586300370040","url":null,"abstract":"Fetal development in domestic cat can be monitored using ultrasonography to control viability, maintain the nutrition care, and drug use. Due to control the viability of foetuses, this study observed the correlation of gestational sac diameter, fetal head diameter, and fetal heart diameter compared to gestational age. This study was performed by using 9 queens that divided into three groups. The first group measured the gestational sac diameter, the second group measured the fetal head diameter, and the third group measured the fetal heart diameter. Gestational sac diameter and fetal head diameter are measured by specific formula, while the fetal heart diameter calculated by measuring point to point of heart edge and compare to head diameter to verify the gestational age. As the result, the gestational sac diameter, the feal head diameter, and the fetal heart rate of domestic cat in Indonesia have correlation to gestational age and also can be used to estimate the gestational edge.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75839246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：