决明子瘘管的抗微生物、抗氧化潜能及其植物化学评价研究

M. Sharif, F. Ansari, Najamul Hassan
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摘要

决明子是一种开花植物,是豆科植物。摘要本研究旨在研究鸢尾草乙醇提取物的抑菌、抗氧化及植物化学活性。采用盘式扩散法和孔式扩散法,对革兰氏阳性分离株蜡样芽孢杆菌、金黄色葡萄球菌和革兰氏阴性分离株伤寒沙门氏菌、肺炎克雷伯菌、大肠杆菌、铜绿假单胞菌进行抑菌试验。其中,25 mg叶提取物(CF-05)对伤寒沙门菌的抑制区最大,为21mm; 50 mg果提取物(CF-13)对伤寒沙门菌的抑制区最大,为17 mm。定性分析和测定了不同浓度下的抗氧化活性。植物化学分析显示存在生物碱、碳水化合物、脂肪、单宁、类黄酮、皂苷、萜类和甾醇。对2,2 -二苯基-1-吡啶酰肼(DPPH)自由基的清除活性显示了其独特的抗氧化活性。
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Anti-microbial, antioxidant potential of Cassia fistula and study their phytochemical assessments
Cassia fistula (C. fistula) is a flowering plant and a member of Fabaceae family. This study was designed to examine the antibacterial, antioxidant and phytochemical activity of ethanolic extract of C. fistula plant. The microbial inhibitory effect of ethanolic extracts of C. fistula was tested against Gram positive isolates such as Bacillus cereus, Staphylococcus aureus and Gram-negative isolates such as Salmonella typhi, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa using disc diffusion method and well diffusion method. The 25 mg extract of C. fistula leaves (CF-05) showed more zone of inhibition against Salmonella typhi, i.e., (21mm) and in 50 mg extract of CF-13 fruit showed best zone of inhibition against Salmonella typhi, i.e., 17 mm. Qualitative analysis and antioxidant activity at various concentrations was also measured. The phytochemical analysis showed the presence of alkaloids, carbohydrates, fats, tannins, flavonoids, saponins, terpenoids, and sterols. The antioxidant activity in 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity revealed the distinguished antioxidant activity of C. fistula.
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