需氧细菌浓度对其在氧气环境下生存过程的影响

С. Бандеры
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引用次数: 0

摘要

本研究的目的是研究好氧微生物在不同初始含量的氧气环境中的生存能力。比较气体对单位体积水中不同浓度细菌的影响。以蜡样芽孢杆菌属需氧菌为研究对象。模型水介质是在蒸馏脱氧水的基础上,加入特定类型的细菌。在整个过程中,氧气以0.2 cm3/s的速率冒泡进入微生物水中。研究时间为2小时,总用气量为1.4 dm3。通过计数培养皿上生长的菌落来测定实验前后的微生物数量。在所有实验中,有氧细菌暴露于氧气的两个阶段过程-在单位体积水中其数量的积累和减少。在该过程的第一阶段,NM在1800 ~ 3600 s呈上升趋势,随后呈下降趋势(II阶段)。当水中微生物负荷从102 CFU/cm3增加到104 CFU/cm3时,细菌积累过程的持续时间缩短了两倍。研究了水中细菌在低浓度条件下的活跃繁殖,以及在高浓度条件下的活跃还原,这可以解释为在恒定的氧气供应速率下细胞的破坏。阐述了氧对水生培养基中需氧微生物数量变化的影响。研究了氧对水中细菌的作用将其生存过程分为两个阶段:积累阶段(I阶段)和减少阶段(II阶段)。结果表明,细菌在氧气气氛中积累过程的持续时间取决于其在水中的初始数量,即随着水单位体积初始NM的增加,微生物积累阶段的持续时间显著缩短。
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Influence of Aerobic Bacteria Concentration on the Process of its Survival in the Presence of Oxygen
Purpose of the study is to study the viability of aerobic microorganisms in an oxygen atmosphere with different initial content in the aquatic medium. Compare the effect of gas on different concentrations of bacteria per unit volume of the water. Methods. Aerobic bacteria of the genus Bacillus cereus bacteria type were the studied microorganisms. Model aqueous media were created on the basis of distilled deaerated water with the addition of bacteria of a particular type. Oxygen was bubbled into the microbial water throughout the process at a rate of 0.2 cm3/s. The duration of the study was 2 hours, during which the total gas consumption corresponded to 1.4 dm3. The number of microorganisms (NM) before and after the experiments was determined by counting the colonies that grew on the Petri dishes. Results. A two-stage process of oxygen exposure to aerobic bacteria was detected - accumulation and reduction of its number per unit volume of water during all experiments. At the first stage of the process, there was an increase of NM during 1800-3600 s with its subsequent decrease (II stage). With an increase in the microbial load in the water from 102 to 104 CFU/cm3, the duration of the process of bacterial accumulation was decreased in two times. An active reproduction of bacterial cells was investigated at the low concentration of bacteria in the water, and its active reduction - at the high concentration that is explained by cells destruction under conditions of constant supply of oxygen of the established rate. Conclusions. The oxygen influence on the change of the number of aerobic microorganisms in the aquatic medium is explained. It is investigated that the oxygen action on bacteria in the water divides the process of its viability into two stages: accumulation (I stage) and reduction of its number (II stage). It is shown that the duration of the process of bacteria accumulation in the oxygen atmosphere depends on its initial amount in the water, namely with increasing of the initial NM per unit volume of the water, the duration of the stage of microorganisms accumulation decreases significantly.
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