CDH18基因的单核苷酸多态性影响湖羊的生长性状

Tianyi Liu, Yazhen Bi, Jingjing Bao, Mingyu Shang, Wenping Hu, Li Zhang
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摘要

生长性状是绵羊的重要经济性状。遗传多态性对绵羊性状的改良有很大影响。本研究旨在分析钙粘蛋白18 (CDH18)基因多态性对湖羊生长性状的影响。利用Illumina Ovine SNP 50K BeadChip对湖羊CDH18基因的单核苷酸多态性进行了鉴定。在CDH18基因中筛选出5个snp,其中SNP1 (rs423955510)位于外显子,SNP2 (rs412944692)、SNP3 (rs416959317)、SNP4 (rs398980439)和SNP5 (rs428685044)位于内含子。采用实时定量聚合酶链反应分析了CDH18基因在湖羊组织中的表达,并利用生物信息学技术分析了该基因的结构和系统发育。结果显示,SNP1、SNP2、SNP4和SNP5与体重和体尺寸显著相关(p 0.33)。CDH18基因在不同月龄湖羊肌肉组织中表达。断奶和4月龄肌肉组织的相对表达水平较高。生物信息学分析显示SNP1存在于5 '非翻译区,这可能会影响翻译效率。以上结果提示,这些SNP位点可能影响生长性状,可作为改善湖羊生长性能的潜在分子标记,为羊的育种奠定分子基础,加快羊的育种步伐。
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Single nucleotide polymorphisms in the CDH18 gene affect growth traits in Hu sheep
Growth traits are critical economic traits in sheep. Genetic polymorphism has a great influence on the improvement of sheep traits. The aim of this study was to analyze the effect of cadherin 18 (CDH18) gene polymorphisms on growth traits in Hu sheep. The single nucleotide polymorphisms (SNPs) of the CDH18 gene in Hu sheep were identified by Illumina Ovine SNP 50K BeadChip. Five SNPs were screened out within the CDH18 gene, where SNP1 (rs423955510) was located in exon and SNP2 (rs412944692), SNP3 (rs416959317), SNP4 (rs398980439) and SNP5 (rs428685044) were located in intron. The expression of the CDH18 gene in Hu sheep tissue was analyzed using quantitative real‐time polymerase chain reaction, and the structure and phylogeny of the gene were analyzed using bioinformatics techniques. The results showed that SNP1, SNP2, SNP4, and SNP5 were significantly associated with body weight and body size (p < 0.05). Meanwhile, there were strong linkage disequilibrium relationships between SNP1 and SNP2 (r2 > 0.33). The CDH18 gene was expressed in the muscle tissues of Hu sheep at different months. The relative expression levels at weaning and 4‐month muscle tissue were higher. Bioinformatic analysis revealed that SNP1 existed in the 5′ untranslated regions, which might affect the efficiency of translation. The above findings suggested that these SNP loci might affect growth traits and could be regarded as potential molecular markers for improving the growth performance of Hu sheep, which lay a molecular foundation for the breeding of sheep and accelerate the pace of sheep breeding.
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