{"title":"膳食脂质水平对成年大鲤鱼脂质积累和健康状况的影响","authors":"","doi":"10.1016/j.aaf.2023.07.008","DOIUrl":null,"url":null,"abstract":"<div><p>The effects of different dietary lipid levels on lipid accumulation, inflammatory response, serum bio-chemical index and histological features of intestine and hepatopancreas of <em>O.</em> <em>macrolepis</em> was experimentally evaluated in an eight-weeks study. Fish (initial weight 50.11 ± 2.86 g) were fed with five iso-nitrogenous diets (around 390 g/kg protein) varying with lipid level (5%, 7%, 9%, 11%, 13%, being LL5, LL7, LL9, LL11, and LL13 respectively) in triplicates. Results showed that the content of crude lipid in carcass and hepatopancreas were not affected by dietary lipid levels (<em>P ></em> 0.05). Serum ALT, TP, HDL-c and MDA, etc., were not significantly affected by diets (<em>P ></em> 0.05), while serum total antioxidant capacity in LL9 and LL11 groups were significantly higher than the other groups (<em>P <</em> 0.05). Histological features of hepatopancreas and intestine showed no significant difference among the five diets (<em>P ></em> 0.05), while the height of intestine villus showed the higher trend in LL9 compared with other groups. The relative expression of lipid metabolism related genes (<em>ppar α</em>, <em>cpt-1α</em>, <em>fas,</em> and <em>hsl</em>) and immune response related genes (<em>tlr 22</em>, <em>nrf 2</em>, <em>tnfα,</em> and <em>il-γ</em>) in the hepatopancreas of fish fed diets differing in their crude lipid levels were not significantly different (<em>P ></em> 0.05). The results suggested that a proper dietary lipid level of 9%–11% could maintain higher antioxidant and health status of adult <em>O.macrolepis</em>.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468550X23000990/pdfft?md5=56ced0f7a0bed700ce09df77e324afb3&pid=1-s2.0-S2468550X23000990-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Effects of dietary lipid levels on lipid accumulation and health status of adult Onychostoma macrolepis\",\"authors\":\"\",\"doi\":\"10.1016/j.aaf.2023.07.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The effects of different dietary lipid levels on lipid accumulation, inflammatory response, serum bio-chemical index and histological features of intestine and hepatopancreas of <em>O.</em> <em>macrolepis</em> was experimentally evaluated in an eight-weeks study. Fish (initial weight 50.11 ± 2.86 g) were fed with five iso-nitrogenous diets (around 390 g/kg protein) varying with lipid level (5%, 7%, 9%, 11%, 13%, being LL5, LL7, LL9, LL11, and LL13 respectively) in triplicates. Results showed that the content of crude lipid in carcass and hepatopancreas were not affected by dietary lipid levels (<em>P ></em> 0.05). Serum ALT, TP, HDL-c and MDA, etc., were not significantly affected by diets (<em>P ></em> 0.05), while serum total antioxidant capacity in LL9 and LL11 groups were significantly higher than the other groups (<em>P <</em> 0.05). Histological features of hepatopancreas and intestine showed no significant difference among the five diets (<em>P ></em> 0.05), while the height of intestine villus showed the higher trend in LL9 compared with other groups. The relative expression of lipid metabolism related genes (<em>ppar α</em>, <em>cpt-1α</em>, <em>fas,</em> and <em>hsl</em>) and immune response related genes (<em>tlr 22</em>, <em>nrf 2</em>, <em>tnfα,</em> and <em>il-γ</em>) in the hepatopancreas of fish fed diets differing in their crude lipid levels were not significantly different (<em>P ></em> 0.05). The results suggested that a proper dietary lipid level of 9%–11% could maintain higher antioxidant and health status of adult <em>O.macrolepis</em>.</p></div>\",\"PeriodicalId\":36894,\"journal\":{\"name\":\"Aquaculture and Fisheries\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-08-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2468550X23000990/pdfft?md5=56ced0f7a0bed700ce09df77e324afb3&pid=1-s2.0-S2468550X23000990-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquaculture and Fisheries\",\"FirstCategoryId\":\"1091\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2468550X23000990\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture and Fisheries","FirstCategoryId":"1091","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2468550X23000990","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Effects of dietary lipid levels on lipid accumulation and health status of adult Onychostoma macrolepis
The effects of different dietary lipid levels on lipid accumulation, inflammatory response, serum bio-chemical index and histological features of intestine and hepatopancreas of O.macrolepis was experimentally evaluated in an eight-weeks study. Fish (initial weight 50.11 ± 2.86 g) were fed with five iso-nitrogenous diets (around 390 g/kg protein) varying with lipid level (5%, 7%, 9%, 11%, 13%, being LL5, LL7, LL9, LL11, and LL13 respectively) in triplicates. Results showed that the content of crude lipid in carcass and hepatopancreas were not affected by dietary lipid levels (P > 0.05). Serum ALT, TP, HDL-c and MDA, etc., were not significantly affected by diets (P > 0.05), while serum total antioxidant capacity in LL9 and LL11 groups were significantly higher than the other groups (P < 0.05). Histological features of hepatopancreas and intestine showed no significant difference among the five diets (P > 0.05), while the height of intestine villus showed the higher trend in LL9 compared with other groups. The relative expression of lipid metabolism related genes (ppar α, cpt-1α, fas, and hsl) and immune response related genes (tlr 22, nrf 2, tnfα, and il-γ) in the hepatopancreas of fish fed diets differing in their crude lipid levels were not significantly different (P > 0.05). The results suggested that a proper dietary lipid level of 9%–11% could maintain higher antioxidant and health status of adult O.macrolepis.