白色念珠菌激活的脾细胞在小鼠乳腺癌模型中促进强免疫反应

Siavash Mashhouri, E. Yarahmadi, S. M. A. Froushani
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摘要

背景:白色念珠菌调节抗原呈递细胞成熟的潜力已经在过去的研究中得到证实。树突状细胞是协调适应性免疫反应的关键调节剂,与髓细胞亚型一起,在抗原向T细胞的呈递中发挥重要作用。本研究的目的是评估加热后的4T1细胞提取物激活的脾细胞和酵母形式的白色念珠菌在体内对乳腺癌生长的影响。方法:将4T1细胞皮下注射于BALB/c雌性小鼠左侧(n=40)。当可触及的肿瘤已经形成时,实验组在一周的过渡期内接种两次免疫,要么用加热的4T1提取物激活脾细胞,要么用灭活的白色念珠菌酵母形式的制剂或两者的结合。第二次注射后,一半的动物(n=20)被安乐死,以研究免疫反应情况。结果:与其他荷瘤小鼠相比,联合给药活化的脾细胞具有良好的生存曲线和较慢的肿瘤发展速度。此外,联合治疗显著增加了脾细胞群中IFN-γ的分泌、呼吸爆发和一氧化氮的产生,相反地减少了IL-4、IL-10和TGF-β的分泌。结论:由于小鼠4T1细胞系与人类乳腺癌晚期相似,我们推测加热后的4T1细胞和酵母样白色念珠菌提取物激活的脾细胞可以减少荷瘤小鼠的肿瘤发展。
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Candida Albicans Activated Splenocytes Promote Strong Immune Responses in a Murine Model of Breast Cancer
Background: The potential of Candida albicans to modulate antigen-presenting cells maturation has been documented in past studies. Dendritic cells are critical modulators in the orchestration of adaptive immune responses alongside myeloid subtypes, which play an important role in the presentation of antigens to T cells. The aim of this study was to evaluate the efficacy of splenocytes activated with the extract of heated 4T1 cells and the yeast form of C. albicans against breast cancer growth in vivo . Methods: 4T1 cells were subcutaneously injected into the left flanks of female BALB/c mice (n=40). At a time when palpable tumors had developed, experimental groups were immunized twice at one-week interim with either activated splenocytes with the extract of heated 4T1 or the killed preparation of yeast form of C. albicans or a combination of the two-One week after the second injection, one-half of animals (n=20) were euthanized to investigate the immune response profile. Results: Administration of activated splenocytes with the combination protocol caused a favorable survival curve and slower rates of tumor development compared to other tumor-bearing mice. Moreover, combination therapy significantly increased the secretion of IFN-γ, respiratory burst and nitric oxide production and conversely diminished the secretion of IL-4, IL-10 and TGF-β in the splenocyte population. Conclusion: Since the murine 4T1 cell line is similar to the final stage of human breast carcinoma, we postulate that activated splenocytes with the extract of heated 4T1 cells and yeast form of C. albicans can reduce tumor development in tumor-bearing mice.
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