利用亲水溶剂对朊芽孢杆菌叶片进行植物化学筛选

Rakesh P. Singh, D. Joshi, R. Bhadauria
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引用次数: 0

摘要

亲水剂被描述为离子有机盐,它们分别通过“盐入”或“盐出”作用来帮助增加或减少溶质在给定溶剂中的溶解度。显示非电解质“盐”的盐被称为“亲水盐”,这种现象被称为“亲水性”。印度大约有45000种植物,集中在东喜马拉雅地区。Barleriaprionitis L.的整株植物采集自纳特瓦拉邦的Shrinathji制药研究所,该植物经R.L. Bhardwaj博士鉴定。对朊芽孢杆菌(Barleria Prionitis L)植物叶片的颜色、形状、大小和表面特征等形态学特征进行了研究。植物材料(1 kg)首先用石油醚脱脂,然后用索氏装置用混合物RP-1至RP-6提取。用水浴法蒸发溶剂后,植物提取物的收率约为20g。HPTLC分析结果表明,采用亲水共混液萃取是一种既环保又经济的提取方法。所有在以前的文献中使用有机溶剂报道的植物成分也存在于亲水溶剂中。
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Phytochemical Screening of Barleria Prionitis L Leaves Using Hydrotropic Solvents
Hydrotropic agents are stated as ionic organic salts which help to increase or decrease the solubility of solute in a given solvent via ‘salt in’ or ‘salt out’ effects, respectively. Salts which show ‘salt in’ of non-electrolytes are called “hydrotropic salts” and the phenomenon is known as “hydrotropism. There are about 45,000 plant species in India, with concentrated hotspots in the region of Eastern Himalayas. Entire plants of Barleriaprionitis L. collected from Shrinathji Institute of pharmacy, Nathdwara and the plant was authenticated by Dr. R.L. Bhardwaj. The leaves of Barleria Prionitis L plant was characterized by its morphological features like colour, shape, size and surface characteristics has been studies. The plant materials (1 kg) were initially defatted with petroleum ether and then extracted with blends RP-1 to RP-6 using a Soxhlet apparatus. The yield of the plant extracts measured about 20 g each after evaporating the solvent using water bath. As per result of HPTLC, we found that the hydrotropic blends solution aur ecoferindly and cost effective method for the extraction purpose. All the phytoconstitutents which was earlier reported in previous literature by the use of organic solvents which were also present for the use of hydrotropic solvents.
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