胃肠道间质瘤的免疫应答

V. Herlea, Alex, R. Roşulescu, Andreea Iorgescu, S. Dima, T. Dumitrașcu, V. Brașoveanu, C. Stroescu, CatalinVasilescu, I. Popescu
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引用次数: 2

摘要

背景:胃肠道间质瘤(胃肠道间质瘤)是消化道最常见的间质肿瘤。肿瘤内免疫细胞的评估可以提供有价值的预后信息,并可能有助于开发针对特定病例的靶向免疫疗法。在这里,我们评估了胃肠道间质肿瘤的炎症浸润,细胞毒性和辅助T细胞的比例及其预后意义。方法:回顾性分析25例胃肠道间质瘤合并胃肠外间质瘤的病例。免疫组化检测CD3、CD4、CD8、CD20和CD68,强调免疫细胞。利用ImageJ软件对炎症细胞进行定量分析。统计分析免疫反应与临床病理和预后变量之间的相关性。结果:所有病例均有不同数量的免疫细胞浸润。分布呈弥漫性或聚集性,多见于血管周围。胃肿瘤中炎症浸润最多,egist最低。瘤内免疫细胞以淋巴细胞为主,浆细胞、组织细胞、嗜酸性粒细胞、中性粒细胞和肥大细胞较少。CD3+淋巴细胞是最常见的亚型。9例CD8+/CD4+比值亚单位。组织细胞数量的增加与疾病进展的高风险相关。免疫细胞与其他预后因素之间没有其他相关性。结论:胃肠道间质瘤是多种免疫细胞与肿瘤细胞复杂相互作用的场所。CD68+细胞的积累与高危gist相关。本文对该肿瘤类型的炎症进行了综述,需要进一步的研究以获得更全面的结果。
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The Immune Response in Gastrointestinal Stromal Tumors
Background: Gastrointestinal stromal tumors (GISTs) are the most frequent tumors with mesenchymal origin at the level of the digestive tract. Assessment of intratumoral immune cells can provide valuable prognostic information and may contribute to the development of targeted immune therapies for selected cases. Here in we evaluated the inflammatory infiltrate in gastrointestinal stromal tumors, the ratios between cytotoxic and helper T cells and their prognostic significance. Methods: We retrospectively analysed 25 cases of GISTs and extragastrointestinal stromal tumors (EGISTs). Immunohistochemical testing for CD3, CD4, CD8, CD20 and CD68 was performed to emphasize the immune cells. Inflammatory cells were quantified with the help of ImageJ software. Statistical analysis was performed to search for correlation between the immune response and clinical-pathological and prognostic variables. Results: GISTs were in all cases infiltrated with immune cells in variable amount. The pattern of distribution was diffuse or in aggregates, most frequent around blood vessels. Gastric tumors had the largest amount of inflammatory infiltrate and EGISTs the lowest. The dominant intratumoral immune cells were represented by lymphocytes, with fewer plasma cells, histiocytes, eosinophils, neutrophils and mast cells. CD3+ lymphocytes were the most common subtype. In 9 cases the CD8+/CD4+ ratio was subunitary. An increased number of histiocytes were associated with a high risk of disease progression. No other correlation between immune cells and other prognostic factors were established. Conclusion: Gastrointestinal stromal tumors represent the site of complex interactions between various types of immune cells and neoplastic cells. Accumulation of CD68+ cells correlates with high risk GISTs. Our paper provides an overview on the inflammation in this tumor type and further studies are necessary for more comprehensive results.
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