新硫杆菌氧化硫代硫酸盐的机理

Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation Pub Date : 1966-12-14 DOI:10.1016/0926-6593(66)90012-9

A. Michael Charles , Isamu Suzuki

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引用次数: 94

摘要

1.1. 新硫杆菌(Thiobacillus novellus)的全细胞和无细胞提取物将硫代硫酸盐氧化为硫酸盐，没有中间聚硫酸盐的硫积累，每氧化1mol硫代硫酸盐消耗2mol O2。四硫酸盐被全细胞氧化为硫酸盐，而非细胞提取物则不能。亚硫酸盐被整个细胞和提取物迅速氧化为硫酸盐，每氧化1mol亚硫酸盐消耗0.5 mol O2。实验结果表明，亚硫酸盐氧化酶(亚硫酸盐:细胞色素c氧化还原酶)和细胞色素氧化酶(细胞色素c: O2氧化还原酶，EC 1.9.3.1)催化了氧化反应。单质硫被全细胞氧化为硫酸盐，但被提取物氧化需要添加GSH.5.5。罗丹斯(硫代硫酸盐:氰化物硫转移酶，EC 2.8.1.1)仅在氰化物存在的情况下才能在硫代硫酸盐中还原细胞色素c。本文提出了以下几种反应来催化T. novellus氧化硫代硫酸盐:

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Mechanism of thiosulfate oxidation by Thiobacillus novellus

1.
1. Whole cells and cell-free extracts of Thiobacillus novellus oxidized thiosulfate to sulfate without intermediary accumulation of sulfur of polythionates, consuming 2 moles of O₂ for every mole of thiosulfate oxidized.
2.
2. Tetrathionate was oxidized to sulfate by whole cells, but not by cell-free extracts.
3.
3. Sulfite was rapidly oxidized by whole cells and extracts to sulfate, consuming 0.5 mole of O₂ for every mole of sulfite oxidized. The oxidation was catalyzed by sulfite oxidase (sulfite: cytochrome $c$ oxidoreductase) and cytochrome oxidase (cytochrome $c$ : O₂ oxidoreductase, EC 1.9.3.1) as shown by manometric as well as spectrophotometric experiments.
4.
4. Elemental sulfur was oxidized by whole cells to sulfate, but the oxidation by extracts required the addition of GSH.
5.
5. Rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1) was found in the extracts which reduced cytochrome $c$ within thiosulfate only in the presence of cyanide.
6.
6. The following reactions are proposed for the oxidation of thiosulfate by T. novellus:

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Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation

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