荧光标记DNA切割过程的荧光相关光谱数值分析

Goro Nishimura, Rudolf Rigler, Masataka Kinjo
{"title":"荧光标记DNA切割过程的荧光相关光谱数值分析","authors":"Goro Nishimura,&nbsp;Rudolf Rigler,&nbsp;Masataka Kinjo","doi":"10.1002/1361-6374(199709)5:3<129::AID-BIO6>3.0.CO;2-6","DOIUrl":null,"url":null,"abstract":"<p>The cleaving process of rhodamine labeled DNA with T7-exonuclease was monitored using the fluorescence correlation method. The fluorescence correlation function, formulated so as to take into account the fluorescence efficiency of the fragment, was derived to allow analysis of the two-component system. The reaction was well represented by the two-component model, allowing monitoring of the progressive cleavage of DNA. The analysis yielded not only the size of the polymer fragment but also the efficiency of the incorporation of rhodamine and the ratio of its quantum yield in DNA and in solution.</p>","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199709)5:3<129::AID-BIO6>3.0.CO;2-6","citationCount":"12","resultStr":"{\"title\":\"Number analysis of fluorescence correlation spectroscopy for the cleaving process of fluorescence labeled DNA\",\"authors\":\"Goro Nishimura,&nbsp;Rudolf Rigler,&nbsp;Masataka Kinjo\",\"doi\":\"10.1002/1361-6374(199709)5:3<129::AID-BIO6>3.0.CO;2-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The cleaving process of rhodamine labeled DNA with T7-exonuclease was monitored using the fluorescence correlation method. The fluorescence correlation function, formulated so as to take into account the fluorescence efficiency of the fragment, was derived to allow analysis of the two-component system. The reaction was well represented by the two-component model, allowing monitoring of the progressive cleavage of DNA. The analysis yielded not only the size of the polymer fragment but also the efficiency of the incorporation of rhodamine and the ratio of its quantum yield in DNA and in solution.</p>\",\"PeriodicalId\":100176,\"journal\":{\"name\":\"Bioimaging\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-05-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/1361-6374(199709)5:3<129::AID-BIO6>3.0.CO;2-6\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioimaging\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199709%295%3A3%3C129%3A%3AAID-BIO6%3E3.0.CO%3B2-6\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioimaging","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199709%295%3A3%3C129%3A%3AAID-BIO6%3E3.0.CO%3B2-6","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 12

摘要

采用荧光相关法监测t7外切酶标记的罗丹明DNA的切割过程。推导了考虑片段荧光效率的荧光相关函数,以便对双组分体系进行分析。该反应由双组分模型很好地表示,允许监测DNA的渐进切割。分析不仅得到了聚合物片段的大小,还得到了罗丹明的掺入效率及其在DNA和溶液中的量子产率之比。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Number analysis of fluorescence correlation spectroscopy for the cleaving process of fluorescence labeled DNA

The cleaving process of rhodamine labeled DNA with T7-exonuclease was monitored using the fluorescence correlation method. The fluorescence correlation function, formulated so as to take into account the fluorescence efficiency of the fragment, was derived to allow analysis of the two-component system. The reaction was well represented by the two-component model, allowing monitoring of the progressive cleavage of DNA. The analysis yielded not only the size of the polymer fragment but also the efficiency of the incorporation of rhodamine and the ratio of its quantum yield in DNA and in solution.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Magnetic Particle Imaging Magnetic Resonance Imaging Quantitative evaluation of light microscopes based on image processing techniques Confocal microscopy of single molecules of the green fluorescent protein Heavy metal contrast enhancement for the selective detection of gold particles in electron microscopical sections using electron spectroscopic imaging
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1