葡萄籽提取物和/或水飞蓟素对硫代乙酰胺诱导大鼠肝纤维化的保护作用

S. Nada, Ayah M H Gowifel, E. S. El-Denshary, A. Salama, M. Khalil, K. Ahmed
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引用次数: 25

摘要

本研究旨在评价GSE(100和200 mg/kg)和/或水飞蓟素对taa诱导大鼠肝纤维化的肝保护和抗氧化作用。本研究旨在探讨葡萄籽提取物(GSE)和/或水飞蓟素对硫代乙酰胺(TAA)诱导的大鼠肝纤维化的保护作用。将成年雄性Sprague-Dawley大鼠随机分为7组,每组8只,实验组1为对照组,口服生理盐水;2 ~ 7组大鼠每周2次腹腔注射TAA (100 mg/Kg),连续6周诱导肝纤维化。第二组,作为阳性对照;3 ~ 5组每日口服水飞蓟素(50 mg/kg)、GSE (100 mg/kg)和GSE (200 mg/kg)。6 ~ 7组分别给予水飞蓟素与GSE (100 mg/kg)或GSE (200 mg/kg)联合处理。结果表明,TAA引起肝脏匀浆中羟脯氨酸(Hyp)、丙二醛(MDA)和一氧化氮(NO)含量显著升高,血清转氨酶(AST、ALT)、碱性磷酸酶(ALP)和总胆红素水平升高。而taa单独处理可显著降低血清总蛋白和肝脏匀浆中谷胱甘肽(GSH)含量。GSE(100和200 mg/kg)和/或水飞蓟素可减轻taa诱导的肝纤维化,改善酶活性,降低氧化应激,呈剂量依赖性。GSE(100和200 mg/kg)和/或水飞蓟素同时治疗可不同程度地改善肝组织的组织病理结构。综上所述,GSE (200 mg/kg)与水飞蓟素(50 mg/kg)联合使用具有较强的肝保护作用。
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Protective Effect of Grape Seed Extract and/or Silymarin Against Thioacetamide-induced Hepatic Fibrosis in Rats
The aim of the present study was designed to evaluate the hepatoprotective and antioxidant potentials of GSE (100 and 200 mg/kg) and/or silymarin against TAA-induced liver fibrosis in rats. This study was designed to investigate the protective effect of grape seed extract (GSE) and/or silymarin against thioacetamide (TAA)-induced hepatic fibrosis in Sprague-Dawley rats. Mature male Sprague-Dawley rats were divided into 7 equal groups (8 rats each) and treated as follows: Group 1, kept as control group and orally given saline; groups 2-7 were injected intraperitoneally (i.p.) with TAA (100 mg/Kg) twice weekly for 6 weeks to induce hepatic fibrosis. Group 2, kept as control positive; groups 3-5 were administered daily oral doses of silymarin (50 mg/kg), GSE (100 mg/kg) and GSE (200 mg/kg), respectively. While groups 6-7 were administered combined treatments of silymarin and GSE (100 mg/kg) or GSE (200 mg/kg), respectively. Our results indicated that TAA caused significant elevation of hydroxyproline (Hyp), malondialdehyde (MDA) and nitric oxide (NO) contents in liver homogenate and increased serum levels of: aminotransferases (AST and ALT), alkaline phosphatase (ALP) and total bilirubin. While, TAA-treatment alone significantly decreased serum total protein and reduced glutathione (GSH) content in liver homogenate. Administration of GSE (100 and 200 mg/kg) and/or silymarin attenuated TAA-induced hepatic fibrosis, improved enzymes and reduced the oxidative stress in dose dependant manner Histopathological study showed disruption of the hepatic architecture and collagen fibers deposition in the portal tract of TAA-injected group. Concomitant treatment with GSE (100 and 200 mg/kg) and/or silymarin significantly improved histopathological structure of liver tissue in variable degrees. In conclusion, the combined effect of GSE (200 mg/kg) with silymarin (50 mg/kg) had powerful hepatoprotective effect than any other studied doses.
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