Shinjiro Kobayashi, M. Nomura, Tatsuo Takahashi, Miho Suzuki, R. Nagai, N. Hagino
{"title":"Nε-(羧甲基)赖氨酸诱导的脉络膜血管生成电位促进晚期糖尿病大鼠视网膜新生血管的体外形成","authors":"Shinjiro Kobayashi, M. Nomura, Tatsuo Takahashi, Miho Suzuki, R. Nagai, N. Hagino","doi":"10.2174/1874143600802010079","DOIUrl":null,"url":null,"abstract":"N -(Carboxymethyl)lysine (CML) over-stimulates choroidal neovascularization in vitro in streptozotocin (STZ)-diabetic rat. In this study, we investigated the effects of CML-induced choroidal angiogenic potential on retinal neovascularization during the course of STZ-induced diabetes in rats. Retinal and choroidal explants were isolated from the same eyeball of early-diabetic, advanced-diabetic and age-matched normal rats. When retinal explant was co-cultured with early-diabetic choroidal explant, the number of retinal microvessels was significantly decreased. When retinal ex- plant was co-cultured with advanced-diabetic choroidal explant, the number of retinal microvessels was significantly in- creased. Anti-CML antibody blocked the retinal changes caused by co-culture with both early-diabetic and advanced- diabetic choroidal explant. Antibodies against TNF and VEGF reduced the number of retinal microvessels in the co- culture with advanced-diabetic choroidal explant. These results indicate that the CML-induced choroidal activity is asso- ciated with the angiogenic actions of TNF and VEGF on retinal capillaries in advanced diabetes. During the course of diabetic retinopathy, different actions on retinal neovascularization may operate.","PeriodicalId":22907,"journal":{"name":"The Open Pharmacology Journal","volume":"93 1","pages":"79-86"},"PeriodicalIF":0.0000,"publicationDate":"2008-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Nε-(Carboxymethyl)Lysine-Induced Choroidal Angiogenic Potential Facilitates Retinal Neovascularization in Advanced-Diabetic Rat In Vitro\",\"authors\":\"Shinjiro Kobayashi, M. Nomura, Tatsuo Takahashi, Miho Suzuki, R. Nagai, N. Hagino\",\"doi\":\"10.2174/1874143600802010079\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"N -(Carboxymethyl)lysine (CML) over-stimulates choroidal neovascularization in vitro in streptozotocin (STZ)-diabetic rat. In this study, we investigated the effects of CML-induced choroidal angiogenic potential on retinal neovascularization during the course of STZ-induced diabetes in rats. Retinal and choroidal explants were isolated from the same eyeball of early-diabetic, advanced-diabetic and age-matched normal rats. When retinal explant was co-cultured with early-diabetic choroidal explant, the number of retinal microvessels was significantly decreased. When retinal ex- plant was co-cultured with advanced-diabetic choroidal explant, the number of retinal microvessels was significantly in- creased. Anti-CML antibody blocked the retinal changes caused by co-culture with both early-diabetic and advanced- diabetic choroidal explant. Antibodies against TNF and VEGF reduced the number of retinal microvessels in the co- culture with advanced-diabetic choroidal explant. These results indicate that the CML-induced choroidal activity is asso- ciated with the angiogenic actions of TNF and VEGF on retinal capillaries in advanced diabetes. During the course of diabetic retinopathy, different actions on retinal neovascularization may operate.\",\"PeriodicalId\":22907,\"journal\":{\"name\":\"The Open Pharmacology Journal\",\"volume\":\"93 1\",\"pages\":\"79-86\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-08-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Open Pharmacology Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1874143600802010079\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Pharmacology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874143600802010079","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Nε-(Carboxymethyl)Lysine-Induced Choroidal Angiogenic Potential Facilitates Retinal Neovascularization in Advanced-Diabetic Rat In Vitro
N -(Carboxymethyl)lysine (CML) over-stimulates choroidal neovascularization in vitro in streptozotocin (STZ)-diabetic rat. In this study, we investigated the effects of CML-induced choroidal angiogenic potential on retinal neovascularization during the course of STZ-induced diabetes in rats. Retinal and choroidal explants were isolated from the same eyeball of early-diabetic, advanced-diabetic and age-matched normal rats. When retinal explant was co-cultured with early-diabetic choroidal explant, the number of retinal microvessels was significantly decreased. When retinal ex- plant was co-cultured with advanced-diabetic choroidal explant, the number of retinal microvessels was significantly in- creased. Anti-CML antibody blocked the retinal changes caused by co-culture with both early-diabetic and advanced- diabetic choroidal explant. Antibodies against TNF and VEGF reduced the number of retinal microvessels in the co- culture with advanced-diabetic choroidal explant. These results indicate that the CML-induced choroidal activity is asso- ciated with the angiogenic actions of TNF and VEGF on retinal capillaries in advanced diabetes. During the course of diabetic retinopathy, different actions on retinal neovascularization may operate.