{"title":"pH对两种水稻羟胺、肼和甲磺酸乙酯诱变特异性和诱变效率的影响","authors":"V.J. Augustine, K. Palanichamy, E.A. Siddiq","doi":"10.1016/S0033-7560(75)80026-3","DOIUrl":null,"url":null,"abstract":"<div><p>The influence of treatment pH on the relative mutagenic efficiency and specificity of the base-specific chemicals hydrazine (HZ) and hydroxylamine (HA) in comparison to that of the potent alkylating agent ethyl methanesulfonate (EMS) was studied, under five different pH levels employing two different test systems <em>viz.</em>, the popular rice variety IR-8 and its viable, chlorophyll-deformed mutant, <em>virescent</em>. No mutations were observed in the M<sub>1</sub> generation itself. EMS was found to be the most potent of the three mutagens inducing the highest frequency of mutations under all the pH levels. HZ and HA were more cytotoxic and less mutagenic. However, the mutagenic effects of HZ and HA were much more pronounced in <em>virescent</em> as compared to their effect on IR-8. Thus, the efficiency of the mutagens were found to be dependent on the treatment pH as well as the test systems employed.</p></div>","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1975-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0033-7560(75)80026-3","citationCount":"5","resultStr":"{\"title\":\"Influence of pH on mutagenic specificity and efficiency of hydroxylamine, hydrazine and ethyl methanesulfonate in two rice types\",\"authors\":\"V.J. Augustine, K. Palanichamy, E.A. Siddiq\",\"doi\":\"10.1016/S0033-7560(75)80026-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The influence of treatment pH on the relative mutagenic efficiency and specificity of the base-specific chemicals hydrazine (HZ) and hydroxylamine (HA) in comparison to that of the potent alkylating agent ethyl methanesulfonate (EMS) was studied, under five different pH levels employing two different test systems <em>viz.</em>, the popular rice variety IR-8 and its viable, chlorophyll-deformed mutant, <em>virescent</em>. No mutations were observed in the M<sub>1</sub> generation itself. EMS was found to be the most potent of the three mutagens inducing the highest frequency of mutations under all the pH levels. HZ and HA were more cytotoxic and less mutagenic. However, the mutagenic effects of HZ and HA were much more pronounced in <em>virescent</em> as compared to their effect on IR-8. Thus, the efficiency of the mutagens were found to be dependent on the treatment pH as well as the test systems employed.</p></div>\",\"PeriodicalId\":20794,\"journal\":{\"name\":\"Radiation Botany\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1975-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0033-7560(75)80026-3\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Radiation Botany\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0033756075800263\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiation Botany","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0033756075800263","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Influence of pH on mutagenic specificity and efficiency of hydroxylamine, hydrazine and ethyl methanesulfonate in two rice types
The influence of treatment pH on the relative mutagenic efficiency and specificity of the base-specific chemicals hydrazine (HZ) and hydroxylamine (HA) in comparison to that of the potent alkylating agent ethyl methanesulfonate (EMS) was studied, under five different pH levels employing two different test systems viz., the popular rice variety IR-8 and its viable, chlorophyll-deformed mutant, virescent. No mutations were observed in the M1 generation itself. EMS was found to be the most potent of the three mutagens inducing the highest frequency of mutations under all the pH levels. HZ and HA were more cytotoxic and less mutagenic. However, the mutagenic effects of HZ and HA were much more pronounced in virescent as compared to their effect on IR-8. Thus, the efficiency of the mutagens were found to be dependent on the treatment pH as well as the test systems employed.
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