Determination of the Differences in Penetration of Tigecycline and Vancomycin Into Bronchial Epithelium of Normal and Cystic Fibrosis Lung

Objective: We aimed to investigate the dose and time course of tigecycline and vancomycin for effective intracellular concentration in bronchial epithelium of normal and cystic fibrosis lung. Methods: Differences between vancomycin and tigecycline in terms of their doseand time-dependent intracellular concentrations were determined with in vitro cell culture and the high performance liquid chromatography techniques. Results: The penetration of tigecycline was found significantly higher in cystic fibrosis cells compared to normal bronchial cells. The optimal dose for tigecycline was 30 μg/mL and the optimal time was 8 h in comparison to given dose and penetrating amount into the cells. Vancomycin dose needed to be given for minimal penetration into the cell was extremely higher than the minimum inhibitor concentration described in the guidelines. Conclusions: Penetration of tigecycline into cells of cystic fibrosis lung was found higher than normal lung cells, whereas cell penetration of vancomycin into both normal and cystic fibrosis cells was limited. These data suggest that tigecycline has a high therapeutic potential in lung infections of patients with cystic fibrosis. Klimik Dergisi 2018; 31(2): 139-43.