木香醇叶提取物对白化Wistar大鼠的安全性评价

Q3 Pharmacology, Toxicology and Pharmaceutics Biomedical and Pharmacology Journal Pub Date : 2023-06-30 DOI:10.13005/bpj/2673
J. Raj, P. S, M. K, Srinivasan Ar
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引用次数: 0

摘要

随着自然疗法比现代医学更受青睐的趋势出现,普通人在不知道天然植物产品中含有有毒物质的情况下,食用天然植物产品会面临更大的危险。目的:评价木香叶甲醇提取物对白化Wistar大鼠的安全性。方法:本研究严格按照经济合作与发展组织(OECD)急性毒性研究指南423确定LD50和亚急性毒性研究指南407对试验溶液进行危害识别和风险评估设计。采用草酸法制备甲醇叶提取物。急性毒性试验选用12只12周龄未产未孕Wistar雌性大鼠,平均体重142±2g,每步3只。试验以3只动物300 mg/kg BW的单次试验剂量开始,持续至2000 mg/kg BW。在摄入试验剂量后,每只动物分别在头4小时和以后每天观察毒性迹象,持续2周。亚急性毒性实验选用成年Wistar大鼠30只(每只16周龄,体重250±12g),随机分为3组(1对照组和2研究组),每组10只,雄性5只,雌性5只。对照组给予1%羧甲基纤维素(CMC),剂量为10 ml/kg BW;研究组分别给予500和1000 mg/kg体重(BW)的羧甲基纤维素提取物,连续28 d。随后,所有动物都被处死,并对血液样本进行血液学和生化变化的研究。结果:木香醇叶提取物的致死剂量固定在2000 mg/kg体重以上。摄食习惯、体重、血液学、生化指标及组织病理学无明显变化。结论:木香叶甲醇提取物对白化Wistar大鼠口服是绝对安全的。
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Evaluation of Safety Profile of Costus Pictus D Don Methanolic Leaf Extract on Albino Wistar Rats
With the emerging trend of preferring naturopathy over modern medicine, common people are in more danger when consuming natural plant products without the knowledge of the toxic agents present in them. Aim and Objective: To evaluate the safety of methanolic leaf extract of Costus pictus D Don on albino Wistar rats. Methodology: The current study was designed strictly based on the organization for economic cooperation and development (OECD) guideline 423 for acute toxicity study to determine LD50 and guideline 407 for sub-acute toxicity study for hazard identification and risk assessment of the test solution. Methanolic leaf extract was prepared by soxhlation. 12 animals (each 12-week-old nulliparous, non-pregnant female Wistar rats with a mean weight of 142 ± 2 g), 3 per step were used for the acute toxicity study. The test was initiated with a single test dose of 300 mg/kg BW on three animals and continued till 2000 mg/kg BW. After ingesting the test dose each animal was observed individually for the first 4 hours and later every day for 2 weeks for signs of toxicity. For the sub-acute toxicity study, 30 adult Wistar rats (each 16-week-old rat weighing 250±12g) were randomized into 3 groups (1 control and 2 study groups) of 10 each consisting of five males and 5 females. Animals in the control group received 1% Carboxymethyl cellulose (CMC) at a dose of 10 ml/kg BW whereas the animals in the study group received 500 and 1000 mg/kg body weight (BW) of the extract respectively for 28 days. Later, all the animals were sacrificed and blood samples were studied for hematological and biochemical changes. Results: The lethal dose of Costus pictus D Don methanolic leaf extract was fixed as more than 2000 mg/kg Body weight. No obvious change was observed in feeding habits, weight, hematology, biochemical parameters, and histopathology. Conclusion: Methanolic leaf extract of Costus pictus D Don was observed to be absolutely safe when given orally in albino Wistar rats.
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来源期刊
Biomedical and Pharmacology Journal
Biomedical and Pharmacology Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
1.20
自引率
0.00%
发文量
189
期刊介绍: Biomedical and Pharmacology Journal (BPJ) is an International Peer Reviewed Research Journal in English language whose frequency is quarterly. The journal seeks to promote research, exchange of scientific information, consideration of regulatory mechanisms that affect drug development and utilization, and medical education. BPJ take much care in making your article published without much delay with your kind cooperation and support. Research papers, review articles, short communications, news are welcomed provided they demonstrate new findings of relevance to the field as a whole. All articles will be peer-reviewed and will find a place in Biomedical and Pharmacology Journal based on the merit and innovativeness of the research work. BPJ hopes that Researchers, Research scholars, Academician, Industrialists etc. would make use of this journal for the development of science and technology. Topics of interest include, but are not limited to: Biochemistry Genetics Microbiology and virology Molecular, cellular and cancer biology Neurosciences Pharmacology Drug Discovery Cardiovascular Pharmacology Neuropharmacology Molecular & Cellular Mechanisms Immunology & Inflammation Pharmacy.
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