马来西亚吉兰丹一农场鹌鹑中沙门氏菌的分离及抗生素谱分析

Palanisamy H
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引用次数: 9

摘要

沙门氏菌病是世界各地影响动物和人类的一个重大公共卫生问题。采用粪拭子法、标准沙门氏菌分离技术和标准纸片扩散法进行抗生素敏感性试验,对马来西亚吉兰丹市某商业养殖场鹌鹑中沙门氏菌的流行情况和分离菌株的抗生素谱进行了研究。采用无菌棉签和运输介质对年龄为3周龄和2月龄的鹌鹑90只进行取样,每组45只。结果表明,鹌鹑中沙门氏菌的流行率为11.11% (CI= 6.19, 19.28),所有分离株均对氨苄西林耐药。3周龄雏鸟沙门氏菌感染率与2月龄雏鸟的χ 2 χ 2差异无统计学意义(P < 0.05)。由于与沙门氏菌有关的食物中毒病例不断增加,鹌鹑中沙门氏菌的阳性鉴定可能具有公共卫生意义。沙门氏菌对氨苄西林(人类和动物中常见的一种抗生素)的耐药性增加了对全球人类和动物谨慎使用抗生素的日益增长的呼吁。农场及食物处理人员应始终保持严格的卫生,以保障公众健康。
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Isolation and Antibiogram of Salmonella spp. from Quails in a Farm from Kelantan, Malaysia -
Salmonellosis is a major public health problem around the world affecting both animals and humans. A study was carried out to elucidate the prevalence of Salmonella spp. and antibiogram of the isolates in quails in a commercial farm located in Kelantan, Malaysia using cloacal swabs and standard isolation techniques for Salmonella species and the standard disk diffusion method for the antibiotic sensitivity tests. Ninety quails in two groups of 45 each, aged 3 weeks and 2 months, were sampled using sterile cotton swabs and transport media. The results showed that the prevalence of Salmonella spp. in the quails was 11.11% (CI= 6.19, 19.28) and all the isolates were resistant to ampicillin. There was no significant difference (P>0.05) between the prevalence of Salmonella spp. in birds aged 3 weeks compared with the birds aged 2 months using Chi square at 95% confidence level. The positive identification of Salmonella spp. in quails may have public health implications due to the rising outbreak of Salmonella spp. associated food poisoning cases. The resistance of the Salmonella spp. to ampicillin which is a common antibiotic in man and animals adds weight to the growing call for the prudent use of antibiotics in human and animal populations around the globe. Farms and food handlers should maintain strict hygiene to protect public health at all times.
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