木霉和木腐菌对甘蓝根病原菌的抑制作用并促进白菜植株生长

I. K. Suada, A. Suwastika, I. Pradnyana, N. Shchegolkova, R. Poloskin, O. Gladkov, O. Yakimenko, A. Stepanov
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引用次数: 4

摘要

白菜作物的高经济价值促使农民采取各种措施来抑制对植物有害的棒状根病菌。需要提出的努力必须是环境安全的。一种对环境友好的方法是利用拮抗微生物对病原体进行生物控制。因此,利用像木霉这样被广泛研究的微生物是很重要的,因为它能够抑制棍棒病的发生并促进植物生长。同时,为了最大限度地提高植物生长对植物养分的需求,需要输入木质素等有机物质,以保存土壤养分,改善土壤结构,提高植物对生物和非生物胁迫的抵抗力。前人对该方案的研究发现了一种有效的本地木霉抑制棒根病,因此本研究采用该方案。本研究的目的是寻找一种木霉与木质素的联合治疗方法,以抑制棒根病,促进植物生长。本试验采用2因素、3个重复的随机区组设计。木霉浓度为3个水平,即0个孢子(对照)、1 × 106个孢子。2x106个孢子,每株3x106个孢子,悬浮在150ml水中。木质素处理分别为0.01、0.02、0.05、0.1、0.5、1.0和2%。结果表明:木质素处理对木霉种群数量、病发率、根总干重、根干重有交互作用,对林冠干重无交互作用;以3 × 106孢子(15 g)的木霉种群为最适宜的组合处理,组合比例为0.5%。这一组合导致病害发生率最低,根茎总干重和根干重最低,冠层干重最高。当浓度达到0.5%时,木质素浓度越高,微生物(真菌和细菌)的生长数量越高,但当浓度超过0.5%时,微生物数量下降。
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APPLICATION OF Trichoderma spp. AND LIGNOHUMATE TO SUPPRESS A PATHOGEN OF CLUBROOT (Plasmodiophora brassicae WOR.) AND PROMOTE PLANT GROWTH OF CABBAGE
The high economic value of cabbage crop leads farmers to make various efforts to suppress the pathogen of club root which is detrimental to plants. Efforts that need to be put forward must be environmentally safe. One way that is environmentally friendly is to control a pathogen biologically using antagonistic microbes. Therefore, the use of microbes such as Trichoderma which has been widely studied is important because it was able to suppress clubroot incidence and stimulate plant growth as well. Meanwhile, the need for plant nutrients to maximize plant growth requires an input of organic materials such as lignohumate which preserve soil nutrients, improve soil structure and increase plant resistance to biotic and abiotic stresses. The previous study on this scheme found an effective indigenous Trichoderma to suppress clubroot, therefore it is used in the current study. The objective of this study was to find out a combination treatment of Trichoderma and lignohumate which can suppress clubroot and increase plant growth. This experiment used a Randomized Block Design with 2 factors and 3 replications. Trichoderma concentration consisted of 3 levels, namely 0 spores (control), 1x106 spores. 2x106 spores, and 3x106 spores per plant which were suspended in 150 ml of water. The lignohumate treatments were 0.01, 0.02, 0.05, 0.1, 0.5, 1.0, and 2%. The results showed that lignohumate treatment was interact with Trichoderma population number on disease incidence, total clubroot, root dry weight, but not to canopy dry weight. The most suitable combination of treatments was the Trichoderma population of 3x106 spores (15 g) in combination to lignohumate of 0.5%.  This combination resulted in the lowest disease incidence, the lowest total clubroot, root dry weight, and the highest canopy dry weight. The higher the lignohumate concentration up to 0.5%, the higher the number of microbes (fungi and bacteria) growth, howeverit decreased above the concentration of 0.5%.
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