Pub Date : 2021-09-30DOI: 10.24843/ijbb.2021.v09.i01.p04
N. K. Dewi, I. Wirawan, M. Sritamin
Reishi (Ganoderma lucidum (Curtis) P. Karst) and shiitake (Lentinula edodes (Berk.) Pegler) mushrooms have high economic value and contain bioactive metabolites that provide heatlh benefits. Shiitake mushrooms have a delightfully unique taste as an edible mushroom. Mushroom production can be increased by utilizing microbes known as MGPB (Mushroom Growth Promoting Bacteria). This study aimed to determine the presence of bacteria associated with shiitake and reishi mushrooms in the baglog and determine how these bacteria influence the growth of shiitake and reishi mycelium. Bacteria was isolated from the mushroom baglog then were purified and centrifuged to obtain the cell-free extract. Furthermore, the bacterial cell-free extracts were poured on PDA media, then shiitake and reishi mycelium were inoculated to test the effect of bacteria isolates on fungal growth. The results showed that three isolates have the potential as MGPB in reishi mushroom, i.e., isolate S1C, S1D, and S2A. Reishi mycelium grown on isolate S2A achieved the fastest maximum growth on day 6th. In shiitake mushroom, it was seen that all isolates showed a negative effect on mycelium growth. Shiitake mycelium in isolate S1C, S1D, S2A, and S2B were seen to form a clear zone on PDA medium.
灵芝(Ganoderma lucidum (Curtis) P. Karst)和香菇(Lentinula edodes (Berk.))蘑菇具有很高的经济价值，并含有对健康有益的生物活性代谢物。作为一种可食用的蘑菇，香菇有一种令人愉快的独特味道。蘑菇产量可以通过利用被称为MGPB(蘑菇生长促进菌)的微生物来增加。本研究旨在确定袋子中与香菇和灵芝相关的细菌的存在，并确定这些细菌如何影响香菇和灵芝菌丝体的生长。从蘑菇袋中分离出细菌，然后进行纯化和离心，得到无细胞提取物。将细菌无细胞提取液灌注于PDA培养基上，接种香菇和灵芝菌丝体，观察分离菌对真菌生长的影响。结果表明，3株分离物S1C、S1D和S2A在灵芝中具有作为MGPB的潜力。在分离物S2A上生长的灵芝菌丝体在第6天生长最快。在香菇中，所有菌株对菌丝生长均有负影响。分离的香菇菌丝体S1C、S1D、S2A和S2B在PDA培养基上形成一个清晰的区域。
{"title":"MORPHOLOGICAL IDENTIFICATION OF BACTERIA ISOLATED FROM MUSHROOM BAGLOG AND ITS EFFECT ON SHIITAKE (Lentinula edodes (Berk.) Pegler) AND REISHI (Ganoderma lucidum (Curtis) P. Karst) MYCELIAL GROWTH","authors":"N. K. Dewi, I. Wirawan, M. Sritamin","doi":"10.24843/ijbb.2021.v09.i01.p04","DOIUrl":"https://doi.org/10.24843/ijbb.2021.v09.i01.p04","url":null,"abstract":"Reishi (Ganoderma lucidum (Curtis) P. Karst) and shiitake (Lentinula edodes (Berk.) Pegler) mushrooms have high economic value and contain bioactive metabolites that provide heatlh benefits. Shiitake mushrooms have a delightfully unique taste as an edible mushroom. Mushroom production can be increased by utilizing microbes known as MGPB (Mushroom Growth Promoting Bacteria). This study aimed to determine the presence of bacteria associated with shiitake and reishi mushrooms in the baglog and determine how these bacteria influence the growth of shiitake and reishi mycelium. Bacteria was isolated from the mushroom baglog then were purified and centrifuged to obtain the cell-free extract. Furthermore, the bacterial cell-free extracts were poured on PDA media, then shiitake and reishi mycelium were inoculated to test the effect of bacteria isolates on fungal growth. The results showed that three isolates have the potential as MGPB in reishi mushroom, i.e., isolate S1C, S1D, and S2A. Reishi mycelium grown on isolate S2A achieved the fastest maximum growth on day 6th. In shiitake mushroom, it was seen that all isolates showed a negative effect on mycelium growth. Shiitake mycelium in isolate S1C, S1D, S2A, and S2B were seen to form a clear zone on PDA medium.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73659212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.24843/ijbb.2021.v09.i01.p01
Mohamad Aji Ikhrami, J. Widada, I. D. Puspita, Masagus Muhammad Prima Putra
Streptomyces is a Gram-positive bacteria that produces the largest secondary metabolite compounds. The results of whole-genome sequence analysis showed that Streptomyces can carry more than 30 Biosynthetic Gene Clusters (BGC) encoding secondary metabolites that have the potential to be explored in the exploration for new bioactive compounds. However, not all BGC can be expressed in the laboratory scale and requires a specific activation method. This study aims to explore the potential of chitosan as an elicitor compound to activate and or increase the antibacterial activity of Streptomyces sp. GMR-22 was tested against histamine-producing bacteria (HPB) Morganella morganii TK7 and Citrobacter freundii CK1. Chitosan was added to the fermentation medium with the final concentration of 250, 500, and 750 µg/ml while without the addition of chitosan used as control. Fermentation was carried out for 10 days at room temperature, with constant agitation 200 rpm. The supernatant was separated by centrifugation at 3500 rpm for 15 minutes, then fractionation with ethyl acetate, concentrated by vacuum rotary evaporator, and freeze-dried. The test for antibacterial activity was carried out by the microdilution method with an extract concentration of 100 mg/ml. The test results of the microdilution method showed that the addition of chitosan successfully increases the antibacterial activity with the highest activity shown by the water fraction of 250 µg/ml addition of chitosan which effective in inhibiting the growth of Morganella morganii TK7 and Citrobacter freundii CK1 by 97,29% and 97,92% respectively.
{"title":"THE POTENCY OF CHITOSAN AS AN ELICITOR ON ANTIBACTERIAL ACTIVITY OF Streptomyces sp. GMR-22 AGAINST HISTAMINE-PRODUCING BACTERIA","authors":"Mohamad Aji Ikhrami, J. Widada, I. D. Puspita, Masagus Muhammad Prima Putra","doi":"10.24843/ijbb.2021.v09.i01.p01","DOIUrl":"https://doi.org/10.24843/ijbb.2021.v09.i01.p01","url":null,"abstract":"Streptomyces is a Gram-positive bacteria that produces the largest secondary metabolite compounds. The results of whole-genome sequence analysis showed that Streptomyces can carry more than 30 Biosynthetic Gene Clusters (BGC) encoding secondary metabolites that have the potential to be explored in the exploration for new bioactive compounds. However, not all BGC can be expressed in the laboratory scale and requires a specific activation method. This study aims to explore the potential of chitosan as an elicitor compound to activate and or increase the antibacterial activity of Streptomyces sp. GMR-22 was tested against histamine-producing bacteria (HPB) Morganella morganii TK7 and Citrobacter freundii CK1. Chitosan was added to the fermentation medium with the final concentration of 250, 500, and 750 µg/ml while without the addition of chitosan used as control. Fermentation was carried out for 10 days at room temperature, with constant agitation 200 rpm. The supernatant was separated by centrifugation at 3500 rpm for 15 minutes, then fractionation with ethyl acetate, concentrated by vacuum rotary evaporator, and freeze-dried. The test for antibacterial activity was carried out by the microdilution method with an extract concentration of 100 mg/ml. The test results of the microdilution method showed that the addition of chitosan successfully increases the antibacterial activity with the highest activity shown by the water fraction of 250 µg/ml addition of chitosan which effective in inhibiting the growth of Morganella morganii TK7 and Citrobacter freundii CK1 by 97,29% and 97,92% respectively.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"124 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91298335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.24843/ijbb.2021.v09.i01.p02
Gresia Eldina, I. Wirawan, M. Sritamin
Cymbidium sp. is one of the most popular orchid desired in public, it means high productivity is needed in its fulfillment. One of the obstacle of Cymbidium sp. orchid in order to fullfilment of productivities are the stress and orchid’s seed size which is very small. Vesicular Arbuscular Mycorrhizae is a symbiont fungus that can help absorption of nutrients in stress phase. VAM help Cymbidium sp. to maintenance and increase the productivity. This research aimed to determined VAM species and colonization in rhizospher of Cymbidium sp. orchid. This research has been conducted from November 2020 untill April 2021. Spore isolation was conducted by using wet shievings method, meanwhile VAM colonization was conducted by using roots staining techniques. The result showed there were 4 genus consisted by 6 different type of spores which has been found. There was Glomus (2 types), Dentiscutata (2 types), Acaulospora (1 type), and Gigaspora (1 type). Colonization of VAM in sample plant has been known by presence of VAM special structure, there were vesicular and internal hyphae.
{"title":"MICROSCOPIC IDENTIFICATION OF VESICULAR ARBUSCULAR MYCORRHIZAE (VAM) ASSOCIATED WITH ORCHID Cymbidium sp.","authors":"Gresia Eldina, I. Wirawan, M. Sritamin","doi":"10.24843/ijbb.2021.v09.i01.p02","DOIUrl":"https://doi.org/10.24843/ijbb.2021.v09.i01.p02","url":null,"abstract":"Cymbidium sp. is one of the most popular orchid desired in public, it means high productivity is needed in its fulfillment. One of the obstacle of Cymbidium sp. orchid in order to fullfilment of productivities are the stress and orchid’s seed size which is very small. Vesicular Arbuscular Mycorrhizae is a symbiont fungus that can help absorption of nutrients in stress phase. VAM help Cymbidium sp. to maintenance and increase the productivity. This research aimed to determined VAM species and colonization in rhizospher of Cymbidium sp. orchid. This research has been conducted from November 2020 untill April 2021. Spore isolation was conducted by using wet shievings method, meanwhile VAM colonization was conducted by using roots staining techniques. The result showed there were 4 genus consisted by 6 different type of spores which has been found. There was Glomus (2 types), Dentiscutata (2 types), Acaulospora (1 type), and Gigaspora (1 type). Colonization of VAM in sample plant has been known by presence of VAM special structure, there were vesicular and internal hyphae.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85049462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.24843/ijbb.2021.v09.i01.p06
Shara Yulita Harianja, I. Wirawan, M. Sritamin
Phenol is an organic compound that is toxic to humans and the environment. This compound was contained in the hospital liquid waste and petrochemical-based industrial sectors. This study aims to obtain hydrocarbonoclastic bacterial isolates that act as biodegradation agent in waste handling. Samples obtained from the biofilter tank of liquid waste treatment Bhayangkara Hospital Denpasar,Bali, Indonesia then carried out isolation and inoculation of bacteria in selective media NA (Nutrient Agar) containing Phenol. The bacteria obtained was performed the viability test on the media with various Phenol concentration levels, obtained three isolates of bacteria capable of growing to the concentration Phenol 500 mg / L, i.e. isolates IB2, IB5, and IB10. Therefore, it was performed the degradation ability and rate test on those three isolates. After 72 hours of incubation periods, the change of Phenol concentrations was analyzed using the folinciocalteau method and examined using the spectrophotometer with 660 nm of wavelength. Based on the analysis of final phenol content, the IB2 isolate has the highest degradation capability in the concentration of 600 mg/L (153,4 mg) with a degradation rate of 2,13 mg/h. IB5 isolates have the highest degradation capability in the concentrations of 700 mg/L (109,45 mg) with a degradation rate of 1,52 mg/h. Meanwhile, IB10 isolates show the lowest ability and degradation rate in both concentrations. Through morphological identification and Gram stain, three isolates belong to the Gram-negative rod-shaped bacteria, which has similarities with the Pseudomonas genus.
{"title":"ISOLATION AND ABILITY TEST OF PHENOL-DEGRADING BACTERIA FROM THE LIQUID WASTE IN BHAYANGKARA HOSPITAL, DENPASAR CITY, BALI, INDONESIA","authors":"Shara Yulita Harianja, I. Wirawan, M. Sritamin","doi":"10.24843/ijbb.2021.v09.i01.p06","DOIUrl":"https://doi.org/10.24843/ijbb.2021.v09.i01.p06","url":null,"abstract":"Phenol is an organic compound that is toxic to humans and the environment. This compound was contained in the hospital liquid waste and petrochemical-based industrial sectors. This study aims to obtain hydrocarbonoclastic bacterial isolates that act as biodegradation agent in waste handling. Samples obtained from the biofilter tank of liquid waste treatment Bhayangkara Hospital Denpasar,Bali, Indonesia then carried out isolation and inoculation of bacteria in selective media NA (Nutrient Agar) containing Phenol. The bacteria obtained was performed the viability test on the media with various Phenol concentration levels, obtained three isolates of bacteria capable of growing to the concentration Phenol 500 mg / L, i.e. isolates IB2, IB5, and IB10. Therefore, it was performed the degradation ability and rate test on those three isolates. After 72 hours of incubation periods, the change of Phenol concentrations was analyzed using the folinciocalteau method and examined using the spectrophotometer with 660 nm of wavelength. Based on the analysis of final phenol content, the IB2 isolate has the highest degradation capability in the concentration of 600 mg/L (153,4 mg) with a degradation rate of 2,13 mg/h. IB5 isolates have the highest degradation capability in the concentrations of 700 mg/L (109,45 mg) with a degradation rate of 1,52 mg/h. Meanwhile, IB10 isolates show the lowest ability and degradation rate in both concentrations. Through morphological identification and Gram stain, three isolates belong to the Gram-negative rod-shaped bacteria, which has similarities with the Pseudomonas genus.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88048600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-25DOI: 10.24843/IJBB.2021.V08.I02.P01
A. Novita, Mohammad Basyuni, A. R. Cemda, S. Nora, M. Mariana
In order to alleviate the deleterious effects of salinity, different types of phytohormones have been used. Among them, GA3 has been the main focus of some plant scientists. This study aimed to evaluate the effect of gibberellic acid and salinity on growth and production of roselle (Hibiscus sabdariffa L). This research was conducted in a greenhouse of Agriculture Faculty, University of Sumatera Utara, Medan. The study used utterly randomized design with two factors. The first factor was gibberellic acids. They were gibberellic acid (5 mg L-1) and no gibberellic acid. The second factor was salinity. They were 0 dsm-1 and 4-5 dsm-1. The interaction between gibberellic acid and salinity acid indicated a significant effect on the percentage of germination, chlorophyll a and b and beta-carotene. The application of giberellic acid helped in the tolerance of plants to salinity. Thus, application of GA3 become essential to improve overall productivity in salinity.
{"title":"THE EFFECT of GA3 AND SALINITY on GROWTH of ROSELLE (Hibiscus sabdariffa L.)","authors":"A. Novita, Mohammad Basyuni, A. R. Cemda, S. Nora, M. Mariana","doi":"10.24843/IJBB.2021.V08.I02.P01","DOIUrl":"https://doi.org/10.24843/IJBB.2021.V08.I02.P01","url":null,"abstract":"In order to alleviate the deleterious effects of salinity, different types of phytohormones have been used. Among them, GA3 has been the main focus of some plant scientists. This study aimed to evaluate the effect of gibberellic acid and salinity on growth and production of roselle (Hibiscus sabdariffa L). This research was conducted in a greenhouse of Agriculture Faculty, University of Sumatera Utara, Medan. The study used utterly randomized design with two factors. The first factor was gibberellic acids. They were gibberellic acid (5 mg L-1) and no gibberellic acid. The second factor was salinity. They were 0 dsm-1 and 4-5 dsm-1. The interaction between gibberellic acid and salinity acid indicated a significant effect on the percentage of germination, chlorophyll a and b and beta-carotene. The application of giberellic acid helped in the tolerance of plants to salinity. Thus, application of GA3 become essential to improve overall productivity in salinity.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76329159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-25DOI: 10.24843/IJBB.2021.V08.I02.P04
M. Rupa, L. Lehar, I. Agung
A study was conducted during two planting seasons, namely planting season 1 (PS-1) 2017/2018 and (PS-2): 2018/2019, in Oelnasi Village, Central Kupang sub-district, Kupang District, East Nusa Tenggara, Indonesia. This study aimed to evaluate the rotation pattern of maize with legumes ground covers towards the dynamics of soil carbon and nutrients N, P, K during the two growing seasons in a dryland farming system. The experiment was designed using a randomized block design with 3 (three) treatments replicated five times. The treatments evaluated were: crop rotation patterns consisting of: without rotation (farmer practice) and rotation pattern with Phaseolus lunatus; rotation pattern with Mucuna pruriens. Legume crops planted after harvesting the food crop and allowed to grow during the dry season. Approaching the rainy season, the biomass of the legume crop is returned to the land by immersing them it together with soil cultivation. Chemical fertilizers is given as an independent variables with a dose of 150 kg/ha of Urea; 100 kg/ha of SP36 and 100 kg/ha of KCL. The results showed that the rotation pattern of Mucuna pruriens significantly increased the soil organic C content by 50.56% and legume Phaseolus lunatus by 37.64% to PS-2. The rotation pattern of the legume covers / covered crop also significantly increased the efficiency of agronomic nutrients (N, P, and K nutrients) and maize yields during the two growing seasons. On the other hand, the farmer pattern (without rotation) showed a decrease in soil carbon to PS-2 which of course affect the efficiency of nutrient absorption.
{"title":"DYNAMICS OF SOIL CARBON AND N, P, K NUTRIENTS ON CORN PLANTING PATTERN IN DRY LAND","authors":"M. Rupa, L. Lehar, I. Agung","doi":"10.24843/IJBB.2021.V08.I02.P04","DOIUrl":"https://doi.org/10.24843/IJBB.2021.V08.I02.P04","url":null,"abstract":"A study was conducted during two planting seasons, namely planting season 1 (PS-1) 2017/2018 and (PS-2): 2018/2019, in Oelnasi Village, Central Kupang sub-district, Kupang District, East Nusa Tenggara, Indonesia. This study aimed to evaluate the rotation pattern of maize with legumes ground covers towards the dynamics of soil carbon and nutrients N, P, K during the two growing seasons in a dryland farming system. The experiment was designed using a randomized block design with 3 (three) treatments replicated five times. The treatments evaluated were: crop rotation patterns consisting of: without rotation (farmer practice) and rotation pattern with Phaseolus lunatus; rotation pattern with Mucuna pruriens. Legume crops planted after harvesting the food crop and allowed to grow during the dry season. Approaching the rainy season, the biomass of the legume crop is returned to the land by immersing them it together with soil cultivation. Chemical fertilizers is given as an independent variables with a dose of 150 kg/ha of Urea; 100 kg/ha of SP36 and 100 kg/ha of KCL. The results showed that the rotation pattern of Mucuna pruriens significantly increased the soil organic C content by 50.56% and legume Phaseolus lunatus by 37.64% to PS-2. The rotation pattern of the legume covers / covered crop also significantly increased the efficiency of agronomic nutrients (N, P, and K nutrients) and maize yields during the two growing seasons. On the other hand, the farmer pattern (without rotation) showed a decrease in soil carbon to PS-2 which of course affect the efficiency of nutrient absorption.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83632191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-25DOI: 10.24843/IJBB.2021.V08.I02.P03
Ainul Haq, R. Ayunda, R. L. Puspitasari
This chicken slaughterhouse in Jampang Village is a home industry that is engaged in chicken slaughter. The problem in this research is the process of slaughtering chickens that do not meet the standards. The purpose of this study was to identify the hygiene and sanitation of chicken slaughterhouses. Data collection was done by filling out questionnaires and observations. The observation sheet is made based on SNI-01-6160-1999 concerning Poultry Slaughterhouses (Badan Standarisasi Nasional, 1999). The observation sheet was used to assess the hygiene and sanitation conditions using the scoring method. This study used descriptive statistics to analyze the abserved data. The scoring method is done by determining the weight for each variable and determining the value for each of the assessed aspects. The results showed that the workers were in good health. Not yet using boots, masks, aprons and headgear. The building is still integrated with the residence. The layout is not in line with the flow of the production process. Sewerage drain is readily available. The cage has never been washed using water and detergent. The conclusion is that the hygiene of the chicken cutters and the sanitation of the buildings still do not meet the requirements. The proposal for improvement is the health of chicken slicers and the use of equipment are things that need to be considered, the building must be separated from the residence, the layout should be improved in the direction of the production process, the building construction is made rodent-free, the chicken coop must be cleaned regularly with water and detergent.
{"title":"ANALYSIS OF HYGIENE AND SANITATION IN CHICKEN SLAUGHTERHOUSES IN JAMPANG VILLAGE, BOGOR REGENCY, WEST JAVA PROVINCE, INDONESIA","authors":"Ainul Haq, R. Ayunda, R. L. Puspitasari","doi":"10.24843/IJBB.2021.V08.I02.P03","DOIUrl":"https://doi.org/10.24843/IJBB.2021.V08.I02.P03","url":null,"abstract":"This chicken slaughterhouse in Jampang Village is a home industry that is engaged in chicken slaughter. The problem in this research is the process of slaughtering chickens that do not meet the standards. The purpose of this study was to identify the hygiene and sanitation of chicken slaughterhouses. Data collection was done by filling out questionnaires and observations. The observation sheet is made based on SNI-01-6160-1999 concerning Poultry Slaughterhouses (Badan Standarisasi Nasional, 1999). The observation sheet was used to assess the hygiene and sanitation conditions using the scoring method. This study used descriptive statistics to analyze the abserved data. The scoring method is done by determining the weight for each variable and determining the value for each of the assessed aspects. The results showed that the workers were in good health. Not yet using boots, masks, aprons and headgear. The building is still integrated with the residence. The layout is not in line with the flow of the production process. Sewerage drain is readily available. The cage has never been washed using water and detergent. The conclusion is that the hygiene of the chicken cutters and the sanitation of the buildings still do not meet the requirements. The proposal for improvement is the health of chicken slicers and the use of equipment are things that need to be considered, the building must be separated from the residence, the layout should be improved in the direction of the production process, the building construction is made rodent-free, the chicken coop must be cleaned regularly with water and detergent.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"51 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72556581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-25DOI: 10.24843/IJBB.2021.V08.I02.P05
I. M. P. N. Mahatya, I. Wirawan, I. K. Suada
Juwet (Syzygium cumini L. Skeels) is one of Indonesia's local fruits which its existence is hard to find now. All parts of this plant can be used for many treatments, one of which is as an antioxidant. The purpose of this study was to determine the compounds contained in the fruit and to determine the antioxidant activity as well. Phytochemical analysis was performed using the gas chromatography while antioxidant activity tests were carried out quantitatively by DPPH method. The results showed that the juwet fruit was contained phenol 284.47 mg/100 g GAE, flavonoids 379.07 mg/100g, tannins 3888.67 mg/100 g TAE and anthocyanins 40.28 mg/100 g which consisted of 66 types of chemical compounds. The results of the antioxidant analysis showed that the ethanolic extract of juwet fruit could inhibit DPPH radicals with IC50 of 72,96 µg/ml.
Juwet (Syzygium cumini L. Skeels)是印度尼西亚的一种当地水果，现在很难找到它的存在。这种植物的所有部分都可以用于许多治疗，其中之一是作为抗氧化剂。本研究的目的是确定水果中所含的化合物，并确定其抗氧化活性。采用气相色谱法进行植物化学分析，DPPH法进行抗氧化活性定量测定。结果表明，黄酮类化合物含量为379.07 mg/100g，单宁3888.67 mg/100g，花青素40.28 mg/100g，共66种化合物。抗氧化分析结果表明，柑桔醇提物对DPPH自由基的抑制作用IC50分别为72、96µg/ml。
{"title":"PHYTOCHEMICAL IDENTIFICATION AND ANTIOXIDANT ACTIVITY OF JUWET FRUIT (Syzygium cumini L. Skeels) ETHANOLIC EXTRACT","authors":"I. M. P. N. Mahatya, I. Wirawan, I. K. Suada","doi":"10.24843/IJBB.2021.V08.I02.P05","DOIUrl":"https://doi.org/10.24843/IJBB.2021.V08.I02.P05","url":null,"abstract":"Juwet (Syzygium cumini L. Skeels) is one of Indonesia's local fruits which its existence is hard to find now. All parts of this plant can be used for many treatments, one of which is as an antioxidant. The purpose of this study was to determine the compounds contained in the fruit and to determine the antioxidant activity as well. Phytochemical analysis was performed using the gas chromatography while antioxidant activity tests were carried out quantitatively by DPPH method. The results showed that the juwet fruit was contained phenol 284.47 mg/100 g GAE, flavonoids 379.07 mg/100g, tannins 3888.67 mg/100 g TAE and anthocyanins 40.28 mg/100 g which consisted of 66 types of chemical compounds. The results of the antioxidant analysis showed that the ethanolic extract of juwet fruit could inhibit DPPH radicals with IC50 of 72,96 µg/ml.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74578125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-25DOI: 10.24843/IJBB.2021.V08.I02.P07
I. D. M. Arthagama, I. Dana, P. Wiguna
This research was a potted experiment carried out in a special orchid house (arnet) at the Padang Galleria Padangsambian Kelod from September to November 2019, the aim was to determine the effect of plant media and the provision of liquid organic fertilizers on the growth of dendrobium orchids. The factorial CRD pattern was the design used in this study, which consists of two factors, first, the use of growing media of wood charcoal (K), fern stems (P), and a mixture of fern stems with wood charcoal (M) and, second, the provision of liquid organic fertilizer, namely, Dewa (D), DI Grow (I), and GDM (G). The results showed that the interaction between the use of planting media and the application of liquid organic fertilizer had no significant effect on all plant growth parameters such as plant height, number of leaves, and number of tillers. However, the plant medium as a single treatment had a significant effect on the number of orchid tillers, fern stems (P) as the plant medium gave the highest number of tillers, namely: 2.22 stems or 32.93% more than the growing media for wood charcoal (K) and a mixture of charcoal, wood and fern stems (M) with 1.67 tillers each
{"title":"EFFECT OF VARIOUS TYPES OF GROWING MEDIA AND APPLICATION OF LIQUID ORGANIC FERTILIZER ON THE GROWTH OF DENDROBIUM ORCHIDS","authors":"I. D. M. Arthagama, I. Dana, P. Wiguna","doi":"10.24843/IJBB.2021.V08.I02.P07","DOIUrl":"https://doi.org/10.24843/IJBB.2021.V08.I02.P07","url":null,"abstract":"This research was a potted experiment carried out in a special orchid house (arnet) at the Padang Galleria Padangsambian Kelod from September to November 2019, the aim was to determine the effect of plant media and the provision of liquid organic fertilizers on the growth of dendrobium orchids. The factorial CRD pattern was the design used in this study, which consists of two factors, first, the use of growing media of wood charcoal (K), fern stems (P), and a mixture of fern stems with wood charcoal (M) and, second, the provision of liquid organic fertilizer, namely, Dewa (D), DI Grow (I), and GDM (G). The results showed that the interaction between the use of planting media and the application of liquid organic fertilizer had no significant effect on all plant growth parameters such as plant height, number of leaves, and number of tillers. However, the plant medium as a single treatment had a significant effect on the number of orchid tillers, fern stems (P) as the plant medium gave the highest number of tillers, namely: 2.22 stems or 32.93% more than the growing media for wood charcoal (K) and a mixture of charcoal, wood and fern stems (M) with 1.67 tillers each","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91486108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-25DOI: 10.24843/IJBB.2021.V08.I02.P08
L. L. Komara, N. P. Kurniawan
Kutai National Park is the conservation forest in East Kalimantan, which can be used for community welfare through non-timber forest products utilization. One of the superior commoditty of non-timber forest products in Kandolo Village under the guidance of Kutai National Park is Genjah Sugar Palm (Arenga pinata Merr). This research objective was to determine the morphology, productivity and quality of the east kutai native genjah sugar palm. The materials used are fresh sap, the method of making palm sugar was by heating for 3 hours to evaporate the water. The heating is ended after it becomes thickened. After thickening, the stew is immediately removed from the heat. Before printing the thick sap is stirred so the heat is more evenly distributed. The result of research shows that the morphology of genjah sugar palm is very unique, because it has a short tree shape and a production age of 5-6 years. The productivity was quite high, the yield of sap is more than 12 liters day-1 , the duration of tapping is > 3 months tree1 . The chemical compound quality of genjah palm sugar had water content, ash, sacrose, reducing sugar, copper and mercury content of 2.24%, 1.76%, 95.48%, 1.02%, 1.62 ppm and 0.01 ppm
{"title":"MORPHOLOGY, PRODUCTIVITY AND QUALITY OF GENJAH SUGAR PALM IN KUTAI NATIONAL PARK, EAST KALIMANTAN, INDONESIA","authors":"L. L. Komara, N. P. Kurniawan","doi":"10.24843/IJBB.2021.V08.I02.P08","DOIUrl":"https://doi.org/10.24843/IJBB.2021.V08.I02.P08","url":null,"abstract":"Kutai National Park is the conservation forest in East Kalimantan, which can be used for community welfare through non-timber forest products utilization. One of the superior commoditty of non-timber forest products in Kandolo Village under the guidance of Kutai National Park is Genjah Sugar Palm (Arenga pinata Merr). This research objective was to determine the morphology, productivity and quality of the east kutai native genjah sugar palm. The materials used are fresh sap, the method of making palm sugar was by heating for 3 hours to evaporate the water. The heating is ended after it becomes thickened. After thickening, the stew is immediately removed from the heat. Before printing the thick sap is stirred so the heat is more evenly distributed. The result of research shows that the morphology of genjah sugar palm is very unique, because it has a short tree shape and a production age of 5-6 years. The productivity was quite high, the yield of sap is more than 12 liters day-1 , the duration of tapping is > 3 months tree1 . The chemical compound quality of genjah palm sugar had water content, ash, sacrose, reducing sugar, copper and mercury content of 2.24%, 1.76%, 95.48%, 1.02%, 1.62 ppm and 0.01 ppm","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73676674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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