中性粒细胞活化下活性氧与半胱氨酸的相互作用

V. Lutsenko, D. Grigorieva, I. Gorudko, A. V. Sokolov, O. Panasenko, S. Cherenkevich
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引用次数: 1

摘要

活性氧(ROS)在人体生理和病理生理过程中起着至关重要的作用。长期暴露于高浓度ROS可能导致心血管、神经退行性等疾病。在这项研究中,半胱氨酸被提议登记ROS的产生。分析了半胱氨酸在活性氧(•О2)、H2O2和活性卤素(HOCl)下光谱性质的变化。结果表明,在活性氧和活性卤素的作用下,染料在溶液中被氧化。根据获得的数据,可以认为超氧阴离子自由基对活化中性粒细胞悬浮液中染料的化学转化起主要作用。这是因为半胱氨酸可以用来评估中性粒细胞,即nadph氧化酶的功能活性,以及设计和测试与氧化应激相关疾病的新型治疗剂。
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Interaction between reactive oxygen species and gallocyanine under neutrophil activation
Reactive oxygen species (ROS) have a crucial role in human physiological and pathophysiological processes. Prolonged exposure to high ROS concentrations may lead to cardiovascular, neurodegenerative, etc. diseases. In this study, gallocyanine has been proposed to register the ROS production. The gallocyanine spectral properties changes under ROS (•О2ˉ, H2O2) and reactive halogen (HOCl) species are analyzed. It is shown that the dye is oxidized in solution, both under the action of ROS and reactive halogen species. Based on the data obtained, it is possible to suggest that superoxide anion radicals make a major contribution to chemical conversion of the dye in suspensions of activated neutrophils. It is that gallocyanine can be used to assess the functional activity of neutrophils, namely, the NADPH-oxidase, as well as to design and test novel therapeutic agents for diseases associated with developing oxidative stress.
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