不同木薯渣混合栽培对普通小球藻生长的影响

N. Un, Agwa Ok
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引用次数: 2

摘要

由于木薯废料不加选择地和持续地倾倒给环境造成环境危害,因此必须将其回收利用成各种生物制品。这种开发利用了它们作为生物质发电微藻原料的潜力。木薯废弃物的近似组成为:木薯皮碳水化合物含量为86.85%,蛋白质含量为4.18%,脂肪含量为5.98%;木薯消化液碳水化合物含量为75%,蛋白质含量为2.5%,脂肪含量为7.0%。木薯废水的理化含量(mg/ ml)为:pH-3.55, DO- 6.17, BOD5-138.81, COD-246.50, TDS-912.70,硝酸盐-13.41,磷酸盐-21.42,硫酸盐-15.69,钙-17.61,镁-9.56。木薯皮提取物的ph值为:pH-3.58, DO-6.25, BOD5-141.82, COD-151.60, TDS-132.23,硝酸盐-12.30,磷酸盐- 13.14,硫酸盐-18.17,钙-10.44,镁-13.30。在不同浓度比的普通小球藻(Chlorella vulgaris)砧木上培养木薯废混合物,在室温和自然光照下保存14 d。在光密度为670 nm的条件下,所有木薯废料混合物的最佳生长条件为160:40,CP:CW的产量为1.595 (abs), CW:CP的产量为1.416 (abs)。普通小球藻在CP:CW浓度为160:40时的生长速度较好,在第2 ~ 4天呈指数增长,而CW:CP浓度为140:60时的生长速度较好。所有木薯废弃物浓度的最大加倍时间在第6天,CP:CW浓度为100:100和CW:CP浓度为160:40时,第10天和第8天的加倍时间最短。从本研究的结果可以看出,木薯皮水与木薯废水的混合物在不同的浓度下都能支持普通小球藻的生长,但在160:40的浓度下生长最佳,因此在木薯废料上培养普通小球藻可以作为一种补救措施来遏制木薯废料乱倾倒的威胁,这对环境造成了很大的危害。
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Growth Response of Chlorella vulgaris to Cultivation on Different Cassava Waste Mixtures
The environmental hazard caused by the indiscriminate and continuous dumping of cassava wastes to the environment has necessitated the need for their recycling into diverse biological products. This exploitation has harnessed their potential to serve as a microalgae feed stock for biomass generation. Proximate composition of the cassava wastes showed cassava peel: carbohydrate-86.85%, protein-4.18%, lipid 5.98% and cassava digestate Carbohydrate-75%, protein-2.5%, lipid-7.0%. Physiochemical contents of the cassava waste in mg/ ml showed cassava waste water: pH-3.55, DO- 6.17, BOD5-138.81, COD-246.50, TDS-912.70, Nitrate-13.41, Phosphate-21.42, Sulphate-15.69, Calcium-17.61 and magnesium-9.56. Cassava peel extracts showed: pH-3.58, DO-6.25, BOD5-141.82, COD-151.60, TDS-132.23, Nitrate-12.30, Phosphate- 13.14, Sulphate-18.17, Calcium-10.44 and magnesium-13.30. The cassava waste mixtures were cultivated on Chlorella vulgaris stock culture at various concentration ratios for a retention period of 14 days at an ambient temperature and natural illumination. Optimum growth was obtained with 160:40 for all cassava waste mixtures at an Optical density of 670 nm yielding 1.595 (abs) for CP:CW and 1.416 (abs) for CW:CP. The growth rate of Chlorella vulgaris at CP:CW was favourable with 160:40 concentration which increased exponentially from the 2nd to 4th day, while that of CW:CP concentrations was favourable at 140:60. Maximum doubling time for all the cassava waste concentration was observed at the 6th day while the minimum doubling time was observed on the 10th day for CP:CW concentration of 100:100 and on the 8th day for CW:CP concentration of 160:40. From the results obtained from this research, it can be deduced that a mixture of cassava peel water and cassava waste water will support the growth of the Chlorella vulgaris at various concentration mixture though optimal growth is observed at Concentration 160:40 thus the cultivation of Chlorella vulgaris on cassava waste can be exploited as a remedial measure in curbing the menace of indiscriminate dumping of cassava waste which has greatly constituted environmental nuisance.
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