{"title":"整合子I类和II类在产生多重耐药的肺炎克雷伯菌中的流行","authors":"Omar F. H., A. H. Ibrahim","doi":"10.36103/ijas.v51i5.1739","DOIUrl":null,"url":null,"abstract":"This study aimed to determine antibiotic resistance profiles and the distribution of Integron I, Integron II, and genes among uropathogenic Klebsiella pneumoniae isolated from patients suffering from urinary tract infection. The results revealed that one hundred and thirty-seven (91%) out of 150 clinical specimens were Klebsiella pneumoniae. The isolates (137 isolates) were given a higher percentage of resistance against Piperacillin (94.89%)and Rifampicin (93.43%) and the lowest resistance was with Meropenem (40.87%). According to PCR amplification for 15 isolates showed the proportion of class I (intl1) and class II (intl2) integrons were investigated. What are 100% (n=15) and 45% (n=7) of isolates had intl1 and intl2 genes, respectively. PCR products for (Five)isolates were subjected to direct sequencing, and both strands of PCR products were sequenced with an automatic sequencer. These sequences were analyzed for the presence of variance of these genes and the detection of the differences in the nucleotides.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"74 1","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"THE PREVALENCE OF INTEGRON CLASS I AND II AMONG MULTI-DRUG RESISTANCE PRODUCING KLEBSIELLA pneumonia\",\"authors\":\"Omar F. H., A. H. Ibrahim\",\"doi\":\"10.36103/ijas.v51i5.1739\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study aimed to determine antibiotic resistance profiles and the distribution of Integron I, Integron II, and genes among uropathogenic Klebsiella pneumoniae isolated from patients suffering from urinary tract infection. The results revealed that one hundred and thirty-seven (91%) out of 150 clinical specimens were Klebsiella pneumoniae. The isolates (137 isolates) were given a higher percentage of resistance against Piperacillin (94.89%)and Rifampicin (93.43%) and the lowest resistance was with Meropenem (40.87%). According to PCR amplification for 15 isolates showed the proportion of class I (intl1) and class II (intl2) integrons were investigated. What are 100% (n=15) and 45% (n=7) of isolates had intl1 and intl2 genes, respectively. PCR products for (Five)isolates were subjected to direct sequencing, and both strands of PCR products were sequenced with an automatic sequencer. These sequences were analyzed for the presence of variance of these genes and the detection of the differences in the nucleotides.\",\"PeriodicalId\":14562,\"journal\":{\"name\":\"IRAQI JOURNAL OF AGRICULTURAL SCIENCES\",\"volume\":\"74 1\",\"pages\":\"\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2023-06-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IRAQI JOURNAL OF AGRICULTURAL SCIENCES\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.36103/ijas.v51i5.1739\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.36103/ijas.v51i5.1739","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
THE PREVALENCE OF INTEGRON CLASS I AND II AMONG MULTI-DRUG RESISTANCE PRODUCING KLEBSIELLA pneumonia
This study aimed to determine antibiotic resistance profiles and the distribution of Integron I, Integron II, and genes among uropathogenic Klebsiella pneumoniae isolated from patients suffering from urinary tract infection. The results revealed that one hundred and thirty-seven (91%) out of 150 clinical specimens were Klebsiella pneumoniae. The isolates (137 isolates) were given a higher percentage of resistance against Piperacillin (94.89%)and Rifampicin (93.43%) and the lowest resistance was with Meropenem (40.87%). According to PCR amplification for 15 isolates showed the proportion of class I (intl1) and class II (intl2) integrons were investigated. What are 100% (n=15) and 45% (n=7) of isolates had intl1 and intl2 genes, respectively. PCR products for (Five)isolates were subjected to direct sequencing, and both strands of PCR products were sequenced with an automatic sequencer. These sequences were analyzed for the presence of variance of these genes and the detection of the differences in the nucleotides.
期刊介绍：
IRAQI JOURNAL OF AGRICULTURAL SCIENCES (IJAS)is the first agric. scientific and refereed journal established in Iraq. The first volume was published in 1966. IJAS is registered in the number 137 in 1988 of the Baghdad National Library. Years ago, it was published with one issue a year. For the time being, it is published bimonthly (6 issues for a volume). IJAS Deal with: Field Crops. Plant Breeding. Agricultural Economics. Agricultural Extension. Agricultural Mechanization. Basic sciences. Hort. Sciences. Animal Husbandry. Food Technology, Plant Pathology. Plant Entomology. Poultry Sciences. Soil Sciences. Water Resources. Veterinary. Biology.
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