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SYNERGISTIC EFFECT OF SPERM FILTRATION TECHNIQUES AND ANTIOXIDANTS ADDED TO EXTENDER OF IRAQI BUFFALO SEMEN 精子过滤技术与添加到伊拉克水牛精液扩展剂中的抗氧化剂的协同效应
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/kc809146
S. N. Alwaeli, S. M. Eidan, Researcher Prof, Depart, Anim, Coll. Agric. Engin. Sci Prod.
This study investigated the synergistic effect of glass wool and sephadex sperm filtration techniques on improving the low semen quality of buffalo bulls with or without antioxidants. Semen was collected for 14 weeks, evaluated weekly, and divided into 12 groups. Good and low semen have extended using a Tris extender. Good semen quality is divided into three groups [S1: Tris extender; S2: Tris + vitamins E (2 mM) and C (5 mM); S3: Tris + glutamine (20 mM) and arginine (1 mM)]. Low semen quality was divided into three main groups and subdivided into three sub-groups (S4; Tris extender; S5: Tris +vitamins E (2 mM) and C (5 mM); S6: Tris + glutamine (20 mM) and arginine (1 mM)). In the 2nd and 3rd main groups, glass wool and sephadex techniques are used with or without adding vitamins (E+C) and amino acids (Glutamine and arginine) and subdivided into three sub-groups with each technique, referred to as S7–S9 for glass wool and S10–S12 for sephadex techniques. Improving (P<0.01) motility, normal morphology, acrosome, and plasma membrane integrity and reducing total sperm abnormalities of low semen quality were noticed using sephadex with or without adding vitamins or amino acids post-cryopreservation. In conclusion, the sephadex technique removed immotile sperm, dead, abnormal, and good sperm harvested by this technique, which in turn may reflect positively on improving the pregnancy rate.
本研究探讨了玻璃棉和sephadex精子过滤技术在添加或不添加抗氧化剂的情况下对改善水牛公牛低精液质量的协同作用。精液收集 14 周,每周评估一次,分为 12 组。好精液和低精液使用 Tris 扩展剂进行扩展。精液质量好的分为三组 [S1: Tris 扩展剂;S2: Tris + 维生素 E(2 mM)和 C(5 mM);S3:Tris + 谷氨酰胺(20 mM)和精氨酸(1 mM)]。精液质量低分为三大组,又细分为三个亚组(S4:Tris 扩展剂;S5:Tris + 维生素 E(2 mM)和 C(5 mM);S6:Tris + 谷氨酰胺(20 mM)和精氨酸(1 mM)]:三羟甲基氨基甲烷 + 谷氨酰胺(20 毫摩尔)和精氨酸(1 毫摩尔))。在第 2 和第 3 大组中,玻璃棉和 sephadex 技术在添加或不添加维生素(E+C)和氨基酸(谷氨酰胺和精氨酸)的情况下使用,每种技术又分为 3 个小组,玻璃棉技术称为 S7-S9,sephadex 技术称为 S10-S12。结果表明,无论是否添加维生素或氨基酸,使用 sephadex 技术进行冷冻保存后,精子的活力、正常形态、顶体和质膜完整性均有所提高(P<0.01),精液质量低的精子异常总数也有所减少。总之,sephadex 技术可去除不运动精子、死精、畸形精子和通过该技术获得的好精子,这反过来又可对提高妊娠率产生积极影响。
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引用次数: 1
DETECTION OF GENES RESPONSIBLE FOR HEAVY METALS RESISTANCE IN LOCALLY ISOLATED PSEUDOMONAS SPP. 在本地分离的假单胞菌中检测重金属抗性基因
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/wgz9vb91
Al-Sajad M. S H.A.A. Alsalim, Researcher Prof.
Plant growth-promoting rhizobacteria (PGPR) that can tolerate heavy metals, provide the basis for microbial inoculums showing heavy metals tolerance properties. This study was aimed to detect the heavy metal resistance genes in plant-growth-promoting Pseudomonas spp. isolated from many agricultural fields. The collected isolates were screened for their plant growth-promoting (PGP) traits, hydrolytic enzymes, Siderophore, ammonia, and indole-3-acetic acid (IAA). Then, subjected to concentrations of CuSO4, CdCl2, and ZnCl2 to determine the minimum inhibitory concentration (MIC). The DNA was extracted from the selected isolates then PCR test was achieved to detect copA, copB, and czcA genes, responsible for heavy metal resistance. Seventy Pseudomonas spp. isolates were obtained; 41 (58.57%), 6 (8.57%), and 15 (21.42%) isolate produced protease, cellulase, and pectinase, respectively. The isolates were positive for siderophore and ammonia production. However, 68 (97.14%) isolates have produced indole-3-acetic acid. Eight isolates were selected and identified as Pseudomonas aeruginosa using the Vitek 2 compact system. The isolates' resistance to heavy metals differed significantly. The isolate B49 had a higher resistance to CuSO4 (MIC = 3200 µg/ml) and ZnCl2 (MIC = 2600 µg/ml), while the isolate B66 recorded a higher resistance to CdCl2 (MIC = 1000 µg/ml).   copB, and czcA genes were detected in the eight P. aeruginosa isolates, while copA gene was detected in seven, except B69.
能耐受重金属的植物生长促进根瘤菌(PGPR)为微生物接种物提供了耐重金属特性的基础。本研究旨在检测从许多农田中分离出来的促进植物生长假单胞菌中的重金属抗性基因。对收集到的分离菌株进行了植物生长促进(PGP)性状、水解酶、嗜苷酸、氨和吲哚-3-乙酸(IAA)的筛选。然后,用硫酸铜(CuSO4)、氯化镉(CdCl2)和氯化锌(ZnCl2)浓度测定最低抑菌浓度(MIC)。从所选分离菌株中提取 DNA,然后进行 PCR 检测,以检测产生重金属抗性的 copA、copB 和 czcA 基因。共分离出 70 株假单胞菌,其中 41 株(58.57%)、6 株(8.57%)和 15 株(21.42%)分别产生蛋白酶、纤维素酶和果胶酶。这些分离物对嗜苷酸盐和氨的产生呈阳性。但有 68 个(97.14%)分离菌株产生吲哚-3-乙酸。使用 Vitek 2 紧凑型系统筛选出 8 个分离株,并鉴定其为铜绿假单胞菌。分离物对重金属的抗性差异显著。分离物 B49 对 CuSO4(MIC = 3200 µg/ml)和 ZnCl2(MIC = 2600 µg/ml)的抗性较高,而分离物 B66 对 CdCl2(MIC = 1000 µg/ml)的抗性较高。在 8 个铜绿假单胞菌分离物中检测到了 copB 和 czcA 基因,而在 7 个分离物(B69 除外)中检测到了 copA 基因。
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引用次数: 0
ESTIMATION THE EFFECTS OF PLASMA ACTIVATED MEDIUM ON SOME COMPONENTS THAT RELATED WITH GROWING BREAST CANCER CELL LINES 评估血浆活化培养基对乳腺癌细胞株生长相关成分的影响
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/e5wb1502
Rawnaq Z. Fadhil, Mouruj A. Al aubaydi, Kadhim A. Aadim Researcher Prof
This study was aimed to assess the influence of plasma activated medium (PAM) on some parameters of breast cancer cell lines (MCF7 and AMJ13) in comparison to normal adipose-derived stem cells (ASCs). First, nitric oxide (NO) production within the used media (RPMI-1640 and MEM) was examined beyond different exposure time (10,15,20, and 25) minutes directly, and the finding suggested a significant increase in NO with increasing exposure time particularly after 20 and 25 min of irradiation in RPMI, while MEM showed significant results only after 25 min. The metabolic parameters were studied including glucose uptake and lactate dehydrogenase (LDH) activity. The results found a decrease in LDH activity proportional to incubation times, as over incubation to 48 hrs the activity reduced more significantly than the 24 hours with minimal influence on the enzyme activity in the media. Correlated results were demonstrated in glucose results as it’s concentration was elevated in the media with increasing irradiation doses for all three treated cell lines in compared to untreated homologues.
本研究旨在评估血浆活化培养基(PAM)对乳腺癌细胞系(MCF7和AMJ13)与正常脂肪衍生干细胞(ASCs)的一些参数的影响。首先,直接检测了所用培养基(RPMI-1640 和 MEM)在不同照射时间(10、15、20 和 25 分钟)后的一氧化氮(NO)产生情况,结果表明随着照射时间的延长,NO 显著增加,特别是在 RPMI 中照射 20 和 25 分钟后,而 MEM 仅在 25 分钟后才显示出显著结果。研究了代谢参数,包括葡萄糖摄取量和乳酸脱氢酶(LDH)活性。结果发现,LDH 活性的降低与培养时间成正比,培养 48 小时后,LDH 活性的降低比培养 24 小时更明显,而培养基中的酶活性受到的影响很小。葡萄糖结果显示了相关的结果,因为与未处理的同系细胞相比,随着辐照剂量的增加,所有三种处理过的细胞系培养基中的葡萄糖浓度都升高了。
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引用次数: 0
EFFECT OF GLYCEROL ON PERFORMANCE AND SOME BLOOD CHARACTERISTICS OF HOLSTEIN CALVES 甘油对荷斯坦犊牛的性能和某些血液特征的影响
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/731zw966
W. A. Khalid, N. N. Al-Anbari, Researcher Prof, Dept, Animal
This research was to study the effect of adding glycerol at different levels (T1 = control treatment without glycerol, T2 = 75 ml and T3 = 150 ml glycerol) in the body weight, body measurements and some blood characteristics of Holstein calves from 60 to 120 days of calves age. Body weight increased  (P≤0.05) in T3 calves at 80 and 100 days of age, also body weight increased in T2 and T3 calves at 120 days of age. T2 calves were significantly superior (P≤0.01) in average total gains from 60 to 120 days of age. Significantly increased (P≤0.05) at the ages of 100 and 120 days of: Wither height in T3 calves, Body length in T2 calves and Heart girth in T2 and T3 calves. Blood glucose concentration increased (P≤0.05) and blood NEFA concentration decreased (P≤0.01) in T2 calves at 80 days of age. At the age of 100 and 120 days, the concentration of blood glucose was increased (P≤0.01 and P≤0.05) and the concentration of NEFA and BHBA decreased (P≤0.01) in calves of the two treatments T2 and T3. We conclude that the addition of glycerol (75 and 150 ml/day) in the feeding of calves increases body weight, promotes growth and improves blood characteristics by increasing blood glucose and reducing NEFA and BHBA concentrations.
本研究旨在研究添加不同水平甘油(T1 = 不含甘油的对照处理,T2 = 75 毫升和 T3 = 150 毫升甘油)对荷斯坦犊牛 60 至 120 日龄体重、体型和一些血液特征的影响。80 和 100 日龄的 T3 小牛体重增加(P≤0.05),120 日龄的 T2 和 T3 小牛体重也有所增加。从 60 日龄到 120 日龄,T2 犊牛的平均总增重明显高于 T3 犊牛(P≤0.01)。100日龄和120日龄时,T2犊牛的平均总增重明显增加(P≤0.05):T3犊牛的枯高、T2犊牛的体长以及T2和T3犊牛的心围。80日龄时,T2犊牛血糖浓度升高(P≤0.05),血NEFA浓度降低(P≤0.01)。在 100 日龄和 120 日龄时,T2 和 T3 两种处理的犊牛血糖浓度升高(P≤0.01 和 P≤0.05),NEFA 和 BHBA 浓度降低(P≤0.01)。我们的结论是,在犊牛饲粮中添加甘油(75 毫升和 150 毫升/天)可增加体重、促进生长,并通过增加血糖、降低 NEFA 和 BHBA 浓度来改善血液特性。
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引用次数: 0
ENHANCE OF FLAVOR AND SOME MICROBIAL PHYSICOCHEMICAL AND SENSORIAL PROPERTIES OF YOGURT BY DRIED CANTALOUPE PULP 用干哈密瓜果肉改善酸奶的风味和某些微生物理化及感官特性
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/f4pyxy69
Al-Zobaay W. J.M, Al-Zobaay A.H.H, Researcher Assist. Prof. Dept. of Food Sci
This study was aimed to enhance the flavor compounds, improve sensorial, microbial and physicochemical characteristics of the yogurt product using dried cantaloupe pulp. This study included the estimation of three flavor compounds in the yogurt product (acetaldehyde, acetone, and ethanol), after seven day of refrigeraed storage at 4°C, a wide difference in the levels of flavor compounds among the treatments have been shown, the values ​​of acetaldehyde were in the following treatments (A) control treatment, (H) cantaloupe 1 %, (K) cantaloupe3%, as follows 13.46, 14.27, 24.54 mg/L respectively. Acetone values were 8.38, 5.33, 3.47 mg/L respectively. Ethanol were 10.25, 7.12, 4.29 mg/L respectively. Lactobacillus delbrueckii subsp. Bulgaricus log vaible count after the first day of manufacture were 7.38, 8.53, 8.77 cfu ml-1 for A, H and K treatment respectively, gradually decreased through refrigerated storage to reach after 28 days 6.25, 7.41, 7.60 cfu ml-1 respectively. The log vaible count Streptococcus silvarius subsp. Thermophilus at first day of manufacture were 8.20, 9.66, 9.71 cfu ml-1 for A, H and k treatment  respectively, gradually decreased under refrigerated storage to 7.25, 8.50, 8.30 cfu ml-1 respectively after 28 days. Chemical composition did not change, and there were no significant differences among the treatments. Sensory evaluation values showed differences in treatment, the flavor scores for treatments after 28 days of refrigerated storage were (37, 42, 43), the texture were (25, 28, 29), the appearance were  (10,14, 14), color (9, 8, 8) for A, H and k treatment respectively. The treatment K was superior as compared to the other treatments with a score of acceptance (94), compared with the control treatment whose score of acceptance was(81) out to 100
这项研究的目的是利用哈密瓜干果肉提高酸奶产品的风味化合物含量,改善感官、微生物和理化特性。这项研究包括对酸奶产品中的三种风味化合物(乙醛、丙酮和乙醇)进行评估,在 4°C 下冷藏七天后,不同处理中的风味化合物含量差异很大,乙醛值在以下处理中分别为 13.46、14.27 和 24.54 mg/L:(A)对照处理、(H)哈密瓜 1%、(K)哈密瓜 3%。丙酮值分别为 8.38、5.33、3.47 毫克/升。乙醇值分别为 10.25、7.12 和 4.29 毫克/升。保加利亚乳杆菌(Lactobacillus delbrueckii subsp. Bulgaricus)的可检出对数在生产第一天后分别为 7.38、8.53、8.77 cfu ml-1(A、H 和 K 处理),在冷藏储存过程中逐渐下降,28 天后分别为 6.25、7.41、7.60 cfu ml-1。生产第一天,A、H 和 K 处理的嗜热链球菌硅谷亚种的对数值分别为 8.20、9.66 和 9.71 cfu ml-1,在冷藏条件下逐渐下降,28 天后分别为 7.25、8.50 和 8.30 cfu ml-1。化学成分没有变化,各处理之间也没有显著差异。各处理的感官评价值存在差异,冷藏保存 28 天后,A、H 和 k 处理的风味评分分别为(37、42、43),口感评分分别为(25、28、29),外观评分分别为(10、14、14),色泽评分分别为(9、8、8)。与其他处理相比,K 处理的接受度为 94 分,而对照处理的接受度为 81 分(满分 100 分)。
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引用次数: 0
INFLUENCE OF SEX ON GROWTH PERFORMANCE, CARCASS ATTRIBUTES, MEAT QUALITY, AND BLOOD METABOLITES OF AWASSI LAMBS 性别对阿瓦西羔羊生长性能、胴体属性、肉质和血液代谢物的影响
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/tryb9y64
Ja’far Al-Khaza’leh, B. Obeidat
This study evaluated the effect of sex on growth performance, carcass characteristics, meat quality, and blood metabolite parameters of Awassi lambs. Twenty-seven Awassi lambs were allocated into two sex groups: males (n=13) and females (n=14). Lambs were individually penned and fed according to the nutritional needs of small ruminants. The experimental period of the study continued for 63 days, preceded by seven days for dietary and pen adaptation. On day 56 of the experimental period, five lambs of each sex group were randomly chosen and distributed in metabolism chambers to examine the digestibility and nitrogen balance. After 70 days of the trial, all lambs were butchered to determine their carcasses and meat quality. The average dry matter (DM) and crude protein (CP) intakes were significantly affected by sex (p < 0.05). Both sexes had similar DM and CP digestibility. Male lambs had greater nitrogen intake and retention (p < 0.05). Growth performance, weights of fasting animals, weights of hot and cold carcasses, non-carcass parts and carcass cuts were influenced (p < 0.05) by the lambs, sex.
本研究评估了性别对阿瓦西羔羊生长性能、胴体特征、肉质和血液代谢物参数的影响。27 只阿瓦西羔羊被分为两个性别组:雄性(13 只)和雌性(14 只)。羔羊被单独圈养,并根据小型反刍动物的营养需求进行喂养。研究的实验期持续了 63 天,之前有 7 天的饮食和圈舍适应期。在试验期的第 56 天,随机选择每个性别组的五只羔羊,并将其分布在代谢室中,以检测消化率和氮平衡。试验结束 70 天后,将所有羔羊屠宰,测定其胴体和肉质。干物质(DM)和粗蛋白(CP)的平均摄入量受到性别的显著影响(p < 0.05)。雌雄羔羊的干物质(DM)和粗蛋白(CP)消化率相似。雄性羔羊的氮摄入量和保留率更高(p < 0.05)。羔羊的生长性能、禁食动物的体重、热胴体和冷胴体的重量、非胴体部分和胴体切块受羔羊性别的影响(p < 0.05)。
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引用次数: 0
EVALUATION OF THE SMALL FARMERS FUND IN THE AGRICULTURAL INITIATIVE IN IRAQ THROUGH THE COLLECTION EFFICIENCY OF LOANS FOR THE PERIOD 2009 – 2018 通过 2009-2018 年期间贷款的回收效率对伊拉克农业倡议中的小农基金进行评估
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/8508sa16
Sanaa J. Mohammed, M. H. O. A. Khazeli, B. H. Al-Badri
To evaluate and analyze the small farmers fund, one of the funds financed by the allocation of the Supreme Committee for the Agricultural Initiative, this fund finance the unconditioned plastic houses, beekeeping, field crops, winter and summer vegetable crops, protected tomato houses, olive trees services, planting of green silage by fixed sprinkler irrigation and to construct consulting offices. As the total number of loans granted to this fund reached nearly 481 billion dinars in all governorates, amounting to 38991 beneficiaries, constituting about 23% of the total loans disbursed during the study period, Baghdad governorate has 68.6 billion of total amounts of that fund and that consist 16.6% of it, then Anbar came in the second rank by 14.7% then Wasit came in the third rank by 12%, while Kirkuk came in the last. The study reached an analysis of the achievement ratios of the small farmers fund for the period 2009-2018 for all governorates, the provinces of Baghdad and Basra were highest share of this fund  for the year 2009, while the lowest share for these year were for Mysan and Kirkuk.  In 2010, Anbar has the highest share while Kirkuk was the lowest. In 2011-2012, the governorate of Baghdad ranked first, and the lowest were for Kirkuk governorate, in 2013, Anbar came to the first, the lowest was Kirkuk governorate, then Wasit governorate returned to first place with collection rates for 2014 and in 2015 Najaf was the first and for 2016 and 2017, the governorate of Baghdad came first. There is a clear decrease in the rates of late loan repayment in this fund because these loans are closely related to the guarantees that document the loans. Most of the guarantees of these loans are the bill only, whereby the amount of withdrawals loans of all kinds is proportional to the amount of the guarantee on the bill of exchange 40 million dinars. The study recommended the necessity of conducting a field survey of all the purposes that the small farmers fund.
小农基金是由最高农业倡议委员会拨款资助的基金之一,该基金为无条件塑料大棚、养蜂、大田作物、冬夏蔬菜作物、番茄保护棚、橄榄树服务、通过固定喷灌种植青贮饲料以及建设咨询办公室提供资金。该基金在各省发放的贷款总额接近 4810 亿第纳尔,受益人达 38 991 人,约占研究期 间发放贷款总额的 23%,其中巴格达省的贷款总额为 686 亿第纳尔,占基金总额的 16.6%, 安巴尔省以 14.7%位居第二,瓦西特省以 12%位居第三,基尔库克省位居最后。研究分析了 2009-2018 年期间各省小农基金的绩效比率,2009 年巴格达省和巴士拉省的小农基金份额最高,而这两年份额最低的是迈桑省和基尔库克省。 2010 年,安巴尔省所占份额最高,而基尔库克省所占份额最低。2011-2012 年,巴格达省排名第一,基尔库克省排名最低;2013 年,安巴尔省排名第一,基尔库克省排名最低;2014 年,瓦西特省的收款率重回第一;2015 年,纳杰夫省排名第一;2016 年和 2017 年,巴格达省排名第一。该基金的逾期贷款偿还率明显下降,因为这些贷款与记录贷款的担保密切相关。这些贷款的担保大多仅为汇票,各类贷款的提款额与 4000 万第纳尔汇票的担保额成正比。研究建议有必要对小农户资金的所有用途进行实地调查。
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引用次数: 0
PHYTOCHEMICAL DIVERSITY AND NUTRITIONAL VALUE OF KENGER AT DIFFERENT LOCATIONS IN SULAIMANI REGION - IRAQ 伊拉克苏莱曼尼地区不同地点 Kenger 的植物化学多样性和营养价值
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/t7rmmq75
Rozhgar. M. Ahmed
Kenger (Gundelia tournefortii L.) plants were collected based on the availability of these plants in the local market to determine the nutrition value, some phytochemical content, and element content during March 2023 at the vegetative stage at thirteen different locations in the Sulaimani region. Some vegetative traits and chemical contents were studied. The results indicated that the highest content of carbohydrates was (12.670%) at Mawat, the protein was (32.703%) at Hazarmerd, and fiber was (35.620%) at Penjwen locations respectively. The analysis of Kenger edible parts (stem and leaves) through GC-MS revealed the presence of a complex mixture of compounds varying from 13 to 19 compounds among the locations. Compounds such as carboxylic acids ranged from 25.217% at Penjwen to 77.043% at Temar, terpenes ranged from 15.912% in Temar to 57.749% in Penjwen, and sterols ranged from 5.351% at Qara Dagh to 24.688% at Hazarmerd. In other locations, these compounds were detected in between those ranges, with differences in their concentrations. According to the results obtained from ICP-OES analysis, macro-elements (Ca, K, P, S, Mg, and Na) contents between the studied locations, the highest concentration of 3.127% occurred at Temar location, the lowest value was obtained at Gulp location with 2.468%, with an average of 2.811% for all locations.
2023 年 3 月,在苏莱曼尼地区的 13 个不同地点采集了当地市场上的 Kenger(Gundelia tournefortii L.)植物,以确定其营养价值、某些植物化学成分和元素含量。对一些植株性状和化学成分进行了研究。结果表明,马瓦特(Mawat)的碳水化合物含量最高(12.670%),哈扎尔默德(Hazarmerd)的蛋白质含量最高(32.703%),彭杰文(Penjwen)的纤维含量最高(35.620%)。通过气相色谱-质谱对 Kenger 可食用部分(茎和叶)的分析表明,各地存在复杂的化合物混合物,从 13 种到 19 种不等。羧酸类化合物的含量从 Penjwen 的 25.217% 到 Temar 的 77.043%,萜烯类化合物的含量从 Temar 的 15.912% 到 Penjwen 的 57.749%,甾醇类化合物的含量从 Qara Dagh 的 5.351% 到 Hazarmerd 的 24.688%。在其他地点,这些化合物的含量介于上述范围之间,但浓度有所不同。根据 ICP-OES 分析得出的结果,研究地点之间的宏量元素(钙、钾、磷、硒、镁和钠)含量存在差异,最高含量为 3.127%,出现在 Temar,最低含量为 2.468%,所有地点的平均含量为 2.811%。
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引用次数: 0
ISOLATION AND CHARACTERIZATION OF LISTERIA MONOCYTOGENES FROM SOME IRAQI MISCARRIAGE WOMEN 从一些伊拉克流产妇女体内分离出李斯特菌并确定其特征
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/cvh62311
K. K. Ghaima, D. S. Qader, Prof Assist, Instit. of, Biotech. for, Postgrad. Stud
This study was aimed to find out how common Listeria monocytogenes in a group of Iraqi women who had miscarriages. Also, investigates the dependability of molecular diagnosis, specifically evaluating the target hlyA gene as an alternate solution tool for identification. A total of 200 specimens comprising cervical swabs were collected from 176 women suffered from miscarriage and 24 women as control. The detection of L. monocytogenes in miscarriages women by using HiCrome™ Listeria Agar Base as both a selective and differential agar medium for quick and direct recognition of Listeria species. The biochemical tests were achieved for confirmation identification. In addition, virulence-associated genes (hlyA gene) isolates were identified using a Real-time PCR approach. Results show out of the 200 (patients and control) specimens, 176 women with miscarriages were analyzed and it was found that 117 (66.5%) specimens from abortion cases were negative for L. monocytogenes, while 59 (33.5%) specimens were positive for Listeria species. All 24 women of control give negative result. Seventy-five (32.4%) isolates from the 59 positive women showed the presence of the hlyA gene in Real-time PCR. We found that molecular diagnostic through Real-Time PCR is a quick and accurate diagnostic method that can help minimize the time it takes to start a specific treatment and track its success. 
这项研究旨在了解李斯特菌在一群伊拉克流产妇女中的常见程度。同时,研究分子诊断的可靠性,特别是评估目标 hlyA 基因作为识别的替代解决方案工具。研究人员从 176 名流产妇女和 24 名对照组妇女身上共采集了 200 份宫颈拭子标本。使用 HiCrome™ 李斯特菌琼脂培养基作为选择性和鉴别性琼脂培养基,检测流产妇女体内的单核细胞增多症李斯特菌,以快速、直接地识别李斯特菌的种类。生化测试可用于确认鉴定。此外,还使用实时聚合酶链式反应(Real-time PCR)方法鉴定了分离出的毒力相关基因(hlyA 基因)。结果显示,在 200 份(患者和对照组)标本中,对 176 名流产妇女进行了分析,发现 117 份(66.5%)来自流产病例的标本对单核细胞增多症李斯特菌呈阴性,而 59 份(33.5%)标本对李斯特菌呈阳性。所有 24 名对照组妇女的结果均为阴性。在实时聚合酶链式反应(Real-time PCR)中,59 名阳性妇女中的 75 个(32.4%)分离物显示存在 hlyA 基因。我们发现,通过实时 PCR 进行分子诊断是一种快速、准确的诊断方法,有助于最大限度地缩短开始特定治疗和跟踪治疗成功所需的时间。
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引用次数: 0
MOLECULAR CHARACTERIZATION OF M. PHASEOLINA AND ITS MANAGEMENT USING AGROCHEMICALS AND T. HARZIANUM M.Phaseolina的分子特征及其利用农用化学品和T.
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/4p59fb72
K. A. Sido, W. A. Hassan
Systemic Acquired Resistance (SAR) induced by agrochemicals of chitosan (CH) and salicylic acid (SA) at (25, 50 and 100 ppm) , in addition to a biocontrol agent of  T. harzianum (Th) at 4 ×106  were examined against M. phaseolina  the causal agent of charcoal rot of sunflower. The results depended on estimation of diseases severity and microsclerotia density in the soil. Thus, the seeds immersion in CH 75 ppm for 6 h., gave the highest and considerable reduction (p=0.05) in disease severity by 48.25% and reduced microsclerotia survived in the soil up to 70%. Application of SA at 50 and 75 ppm proved an obvious reduction of charcoal rot severity by up to 39% and 37% for both concentrations, respectively and not varied with Th. The results also confirmed that CH at 75 ppm revealed significant reduction 40.63% in disease severity and similarized with SA at same concentration. However, the lowest dose of SA at 25 ppm realized the highest reduction of micro sclerotia density by 80.28 % compared to 74.91% when used CH at 75 ppm. For molecular identification of a pathogen Polymerase Chain Reaction (PCR) using ITS4 and ITS5 universal primers were applied to amplify and sequence of DNA for six isolates of M. phaseolina viz., OL901219, OL636051, OL901220, OL901204, OL636050 and OL636053 compared for identity of rDNA sequence according to NCBI GenBank databases by BLAST mode and the results showed the entire similarity ratio reached to 100%
研究了壳聚糖(CH)和水杨酸(SA)等农用化学品(25、50 和 100 ppm)以及 4 ×106 的向日葵炭腐病病原菌 T. harzianum(Th)生物控制剂诱导的系统获得抗性(SAR)。结果取决于对土壤中病害严重程度和小圆菌体密度的估计。因此,将种子浸泡在 75 ppm 的 CH 中 6 小时,病害严重程度降低了 48.25%(p=0.05),土壤中存活的小圆菌体减少了 70%。事实证明,施用 50 和 75 ppm 的 SA 能明显降低炭腐病的严重程度,两种浓度分别降低 39% 和 37%,且不随 Th 的变化而变化。结果还证实,施用 75 ppm 的 CH 能显著降低病害严重程度 40.63%,与施用相同浓度的 SA 相似。然而,最低剂量的 SA(25 ppm)与 CH(75 ppm)相比,微小硬菌密度减少了 80.28%,而 CH(75 ppm)则减少了 74.91%。为了对病原体进行分子鉴定,使用 ITS4 和 ITS5 通用引物进行聚合酶链式反应 (PCR),以扩增六种相思豆分离物(即 OL901219、OL636051、OL901220、OL901204、OL636050 和 OL636053)的 DNA 序列,并通过 BLAST 模式与 NCBI GenBank 数据库中的 rDNA 序列进行比对,结果显示整个相似率达到 100%。
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引用次数: 0
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IRAQI JOURNAL OF AGRICULTURAL SCIENCES
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