Gergana Marovska, Ivanka Hambarliyska, N. Petkova, Ivan Georgiev Ivanov, I. Vasileva, A. Slavov
{"title":"薰衣草乙醇提取物的化学成分及抗氧化活性研究","authors":"Gergana Marovska, Ivanka Hambarliyska, N. Petkova, Ivan Georgiev Ivanov, I. Vasileva, A. Slavov","doi":"10.56899/152.03.09","DOIUrl":null,"url":null,"abstract":"The objectives of the present study were focused on the comparative study of lavender (Lavandula angustifolia Mill.) raw material and its essential oil industry by-products. The potential of the by-products as a natural source of bioactive polar and nonpolar compounds suitable for applications in the food, cosmetic and pharmaceutical industries was investigated. Four lavender samples were studied – three by-products from industrial steam distillation (L-SD20 M, L-SD21 M, and L-SD21 Z) and one raw untreated lavender (L-UNTR21 Z) – and their chemical composition and antioxidant capacity were determined and compared. Nine phenolic acids (gallic, protocatehuic, chlorogenic, caffeic, syringic, p-coumaric, ferulic, salicylic, and rosmarinic acids) and five flavonoids [(+)-catechin, (–)-epicatechin, rutin, hesperidin, and quercetin] were detected in the 70% ethanolic extract of L-SD20 M. The highest amount of phenolic compounds at 16.08 ± 0.38 mg gallic acid equivalents/ g dry weight (dw) were found in the L-SD21 Z 70% ethanolic extract. L-SD21 M 70% ethanolic extract was distinctive with the highest amounts of flavonoids at 3.89 ± 0.44 mg quercetin equivalents/ g dw. The antioxidant capacity of the extracts was evaluated by four methods: radical scavenging activity of 2,2 diphenyl-1-picrylhydrazyl (DPPH assay), ABTS [2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid)], ferric reducing antioxidant power (FRAP), and cupric ion reducing antioxidant capacity (CUPRAC). The highest antioxidant activity by the CUPRAC method was observed in the 70% ethanolic extract of L-SD21 M – 326.49 millimoles Trolox equivalents/g dw. The major aroma constituents found in the 70% ethanolic extracts of L-UNTR21 Z were linalyl acetate and linalool at 38.64 and 7.89%, respectively. τ-muurolol, an oxidated sesquiterpene, was also found in high amounts in the 70% ethanolic extracts from lavender by-products. Therefore, lavender and its industrially generated by-products could represent a promising source of biologically active and aroma compounds with potent high antioxidant activity.","PeriodicalId":39096,"journal":{"name":"Philippine Journal of Science","volume":"66 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chemical Composition and Antioxidant Activity of Ethanol Extracts Obtained from Lavender (Lavandula angustifolia Mill.)\",\"authors\":\"Gergana Marovska, Ivanka Hambarliyska, N. Petkova, Ivan Georgiev Ivanov, I. Vasileva, A. Slavov\",\"doi\":\"10.56899/152.03.09\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The objectives of the present study were focused on the comparative study of lavender (Lavandula angustifolia Mill.) raw material and its essential oil industry by-products. The potential of the by-products as a natural source of bioactive polar and nonpolar compounds suitable for applications in the food, cosmetic and pharmaceutical industries was investigated. Four lavender samples were studied – three by-products from industrial steam distillation (L-SD20 M, L-SD21 M, and L-SD21 Z) and one raw untreated lavender (L-UNTR21 Z) – and their chemical composition and antioxidant capacity were determined and compared. Nine phenolic acids (gallic, protocatehuic, chlorogenic, caffeic, syringic, p-coumaric, ferulic, salicylic, and rosmarinic acids) and five flavonoids [(+)-catechin, (–)-epicatechin, rutin, hesperidin, and quercetin] were detected in the 70% ethanolic extract of L-SD20 M. The highest amount of phenolic compounds at 16.08 ± 0.38 mg gallic acid equivalents/ g dry weight (dw) were found in the L-SD21 Z 70% ethanolic extract. L-SD21 M 70% ethanolic extract was distinctive with the highest amounts of flavonoids at 3.89 ± 0.44 mg quercetin equivalents/ g dw. The antioxidant capacity of the extracts was evaluated by four methods: radical scavenging activity of 2,2 diphenyl-1-picrylhydrazyl (DPPH assay), ABTS [2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid)], ferric reducing antioxidant power (FRAP), and cupric ion reducing antioxidant capacity (CUPRAC). The highest antioxidant activity by the CUPRAC method was observed in the 70% ethanolic extract of L-SD21 M – 326.49 millimoles Trolox equivalents/g dw. The major aroma constituents found in the 70% ethanolic extracts of L-UNTR21 Z were linalyl acetate and linalool at 38.64 and 7.89%, respectively. τ-muurolol, an oxidated sesquiterpene, was also found in high amounts in the 70% ethanolic extracts from lavender by-products. 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引用次数: 0
摘要
本研究主要对薰衣草原料及其精油工业副产物进行了比较研究。研究了该副产物作为具有生物活性的极性和非极性化合物的天然来源在食品、化妆品和制药工业中的应用潜力。研究了工业蒸汽蒸馏副产物(L-SD20 M、L-SD21 M和L-SD21 Z)和未经处理的薰衣草(L-UNTR21 Z)的4种薰衣草样品,测定了它们的化学成分和抗氧化能力。L-SD20 m 70%乙醇提取物中检测到9种酚酸(没食子酸、原儿茶酸、绿原酸、咖啡酸、丁香酸、对香豆酸、阿魏酸、水杨酸和迷迭香酸)和5种黄酮类化合物[(+)-儿茶素、(-)-表儿茶素、芦丁、橙皮苷和槲皮素],L-SD21 Z 70%乙醇提取物中酚类化合物含量最高,为16.08±0.38 mg没食子酸当量/ g干重(dw)。L-SD21 M 70%乙醇提取物黄酮含量最高,为3.89±0.44 mg槲皮素当量/ g dw。采用DPPH法、ABTS[2,2 ' -氮基-二-(3-乙基苯并噻唑啉磺酸)]清除自由基能力、铁还原抗氧化能力(FRAP)和铜离子还原抗氧化能力(CUPRAC)四种方法评价提取物的抗氧化能力。用CUPRAC法测定,L-SD21 M - 326.49毫摩尔Trolox当量/g dw的70%乙醇提取物的抗氧化活性最高。L-UNTR21 Z 70%乙醇提取物的主要香气成分为乙酸芳樟醇和芳樟醇,分别为38.64%和7.89%。在薰衣草副产品70%乙醇提取物中也发现了大量的氧化倍半萜τ-穆罗尔。因此,薰衣草及其工业副产品可能是一种具有高抗氧化活性的生物活性和芳香化合物的有前途的来源。
Chemical Composition and Antioxidant Activity of Ethanol Extracts Obtained from Lavender (Lavandula angustifolia Mill.)
The objectives of the present study were focused on the comparative study of lavender (Lavandula angustifolia Mill.) raw material and its essential oil industry by-products. The potential of the by-products as a natural source of bioactive polar and nonpolar compounds suitable for applications in the food, cosmetic and pharmaceutical industries was investigated. Four lavender samples were studied – three by-products from industrial steam distillation (L-SD20 M, L-SD21 M, and L-SD21 Z) and one raw untreated lavender (L-UNTR21 Z) – and their chemical composition and antioxidant capacity were determined and compared. Nine phenolic acids (gallic, protocatehuic, chlorogenic, caffeic, syringic, p-coumaric, ferulic, salicylic, and rosmarinic acids) and five flavonoids [(+)-catechin, (–)-epicatechin, rutin, hesperidin, and quercetin] were detected in the 70% ethanolic extract of L-SD20 M. The highest amount of phenolic compounds at 16.08 ± 0.38 mg gallic acid equivalents/ g dry weight (dw) were found in the L-SD21 Z 70% ethanolic extract. L-SD21 M 70% ethanolic extract was distinctive with the highest amounts of flavonoids at 3.89 ± 0.44 mg quercetin equivalents/ g dw. The antioxidant capacity of the extracts was evaluated by four methods: radical scavenging activity of 2,2 diphenyl-1-picrylhydrazyl (DPPH assay), ABTS [2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid)], ferric reducing antioxidant power (FRAP), and cupric ion reducing antioxidant capacity (CUPRAC). The highest antioxidant activity by the CUPRAC method was observed in the 70% ethanolic extract of L-SD21 M – 326.49 millimoles Trolox equivalents/g dw. The major aroma constituents found in the 70% ethanolic extracts of L-UNTR21 Z were linalyl acetate and linalool at 38.64 and 7.89%, respectively. τ-muurolol, an oxidated sesquiterpene, was also found in high amounts in the 70% ethanolic extracts from lavender by-products. Therefore, lavender and its industrially generated by-products could represent a promising source of biologically active and aroma compounds with potent high antioxidant activity.