添加抗氧化剂对公牛冷冻精液补充作用的初步研究

Kassem Houshaimy, D. Țogoe, T. Constantin, C. Micsa, A. Șonea
{"title":"添加抗氧化剂对公牛冷冻精液补充作用的初步研究","authors":"Kassem Houshaimy, D. Țogoe, T. Constantin, C. Micsa, A. Șonea","doi":"10.2478/alife-2018-0067","DOIUrl":null,"url":null,"abstract":"Abstract For a long period of time, cryopreservation and dilution of sperm represented a very important role in improvement of biotechnology of artificial insemination in the bovine. Regarding to this fact the study has been realized in period 2016- 2017 at the center of amelioration for seminal material, Semtest-BVN Târgu-Mureș and at the laboratory of theriogenology of Faculty of veterinary medicine Bucharest. In this study, 7 ml sperm from one Holstein bull (SunnyBoy) was obtained using artificial vagina. The ejaculate was divided to 5 parts and diluted using Triladyl extender (250 g) adding vitamin E with 2 concentrations 1μmol or 2 μmol, catechin 0.1μmol, Trolox 0.1 μmol, and a standard probe without adding antioxidants. Straws had been analyzed using Computer- assisted sperm analysis (CASA) for motility and composite after thawing. As a first conclusion of this study, it can say that the group with vitamin E presented a higher motility than the other groups.","PeriodicalId":6413,"journal":{"name":"“Agriculture for Life, Life for Agriculture” Conference Proceedings","volume":"10 1","pages":"440 - 444"},"PeriodicalIF":0.0000,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preliminary Study Regarding the Additional Effect of Adding Antioxidants on Bull Frozen Semen\",\"authors\":\"Kassem Houshaimy, D. Țogoe, T. Constantin, C. Micsa, A. Șonea\",\"doi\":\"10.2478/alife-2018-0067\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract For a long period of time, cryopreservation and dilution of sperm represented a very important role in improvement of biotechnology of artificial insemination in the bovine. Regarding to this fact the study has been realized in period 2016- 2017 at the center of amelioration for seminal material, Semtest-BVN Târgu-Mureș and at the laboratory of theriogenology of Faculty of veterinary medicine Bucharest. In this study, 7 ml sperm from one Holstein bull (SunnyBoy) was obtained using artificial vagina. The ejaculate was divided to 5 parts and diluted using Triladyl extender (250 g) adding vitamin E with 2 concentrations 1μmol or 2 μmol, catechin 0.1μmol, Trolox 0.1 μmol, and a standard probe without adding antioxidants. Straws had been analyzed using Computer- assisted sperm analysis (CASA) for motility and composite after thawing. As a first conclusion of this study, it can say that the group with vitamin E presented a higher motility than the other groups.\",\"PeriodicalId\":6413,\"journal\":{\"name\":\"“Agriculture for Life, Life for Agriculture” Conference Proceedings\",\"volume\":\"10 1\",\"pages\":\"440 - 444\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"“Agriculture for Life, Life for Agriculture” Conference Proceedings\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2478/alife-2018-0067\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"“Agriculture for Life, Life for Agriculture” Conference Proceedings","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/alife-2018-0067","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

长期以来,精子的冷冻和稀释在牛人工授精的生物技术改进中起着非常重要的作用。鉴于这一事实,该研究于2016年至2017年期间在种子材料改良中心Semtest-BVN t rgu- murebus和布加勒斯特兽医学院兽医学实验室完成。在本研究中,使用人工阴道从一头荷斯坦公牛(SunnyBoy)获得7ml精子。将射精液分成5份,用三甘醇扩展剂(250 g)稀释,分别添加2种浓度的维生素E (1μmol或2 μmol)、儿茶素0.1μmol、Trolox 0.1μmol,标准探针不添加抗氧化剂。用计算机辅助精子分析(CASA)分析了秸秆解冻后的活力和复合成分。作为本研究的第一个结论,可以说维生素E组比其他组表现出更高的运动性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Preliminary Study Regarding the Additional Effect of Adding Antioxidants on Bull Frozen Semen
Abstract For a long period of time, cryopreservation and dilution of sperm represented a very important role in improvement of biotechnology of artificial insemination in the bovine. Regarding to this fact the study has been realized in period 2016- 2017 at the center of amelioration for seminal material, Semtest-BVN Târgu-Mureș and at the laboratory of theriogenology of Faculty of veterinary medicine Bucharest. In this study, 7 ml sperm from one Holstein bull (SunnyBoy) was obtained using artificial vagina. The ejaculate was divided to 5 parts and diluted using Triladyl extender (250 g) adding vitamin E with 2 concentrations 1μmol or 2 μmol, catechin 0.1μmol, Trolox 0.1 μmol, and a standard probe without adding antioxidants. Straws had been analyzed using Computer- assisted sperm analysis (CASA) for motility and composite after thawing. As a first conclusion of this study, it can say that the group with vitamin E presented a higher motility than the other groups.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Experimental Rearing of the Golden Gray Mullet Liza Aurata (Risso, 1810) in a Recirculating System at the Black Sea Еpidemiological Data on Lead Tissue Concentration in Game Birds Induced by Lead Pellets Assessment of Micro and Macro Nutrients Contents in the Turkish Faba Bean Germplasm Effect of Trifluralin, Zero-Valent Iron and Magnetite Nanoparticles on Growth of Micromycetes Sunflower Varieties and Hybrids Registered for Cultivation in Romania in the Last Century
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1