Kassem Houshaimy, D. Țogoe, T. Constantin, C. Micsa, A. Șonea
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引用次数: 0
摘要
长期以来,精子的冷冻和稀释在牛人工授精的生物技术改进中起着非常重要的作用。鉴于这一事实,该研究于2016年至2017年期间在种子材料改良中心Semtest-BVN t rgu- murebus和布加勒斯特兽医学院兽医学实验室完成。在本研究中,使用人工阴道从一头荷斯坦公牛(SunnyBoy)获得7ml精子。将射精液分成5份,用三甘醇扩展剂(250 g)稀释,分别添加2种浓度的维生素E (1μmol或2 μmol)、儿茶素0.1μmol、Trolox 0.1μmol,标准探针不添加抗氧化剂。用计算机辅助精子分析(CASA)分析了秸秆解冻后的活力和复合成分。作为本研究的第一个结论,可以说维生素E组比其他组表现出更高的运动性。
Preliminary Study Regarding the Additional Effect of Adding Antioxidants on Bull Frozen Semen
Abstract For a long period of time, cryopreservation and dilution of sperm represented a very important role in improvement of biotechnology of artificial insemination in the bovine. Regarding to this fact the study has been realized in period 2016- 2017 at the center of amelioration for seminal material, Semtest-BVN Târgu-Mureș and at the laboratory of theriogenology of Faculty of veterinary medicine Bucharest. In this study, 7 ml sperm from one Holstein bull (SunnyBoy) was obtained using artificial vagina. The ejaculate was divided to 5 parts and diluted using Triladyl extender (250 g) adding vitamin E with 2 concentrations 1μmol or 2 μmol, catechin 0.1μmol, Trolox 0.1 μmol, and a standard probe without adding antioxidants. Straws had been analyzed using Computer- assisted sperm analysis (CASA) for motility and composite after thawing. As a first conclusion of this study, it can say that the group with vitamin E presented a higher motility than the other groups.