K. Ibrahim, F. Mohamed, A. Abo-Zeid, M. Elwan, M. Salam
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引用次数: 3
摘要
蛇瓜(Cucumis melo var. flexuosus)原产于从埃及到伊朗的地区;因此,埃及的当地基因型可能包含负责耐受生物和非生物胁迫的基因。利用ISSR (Inter simple sequence repeat)标记对12份来自不同地区的埃及蛇瓜材料进行了亲缘关系分析。10个标记中,6个产生68个扩增产物,其中23个为多态性,多态性为33.7%。6个引物的平均多态性信息含量(PIC)值为0.68。两两相似性系数在0.88 ~ 0.98之间,多样性较窄。聚类分析区分出两个聚类,一个来自开罗,另一个代表来自不同地区的其他加入。结果表明,遗传上相近的蛇瓜基因型在地理距离上并不一定相近。在非编码区水平上,利用6条ISSR引物在埃及蛇瓜材料中发现了一些差异,证实了该技术在检测遗传多样性方面的有效性,这可能有助于未来对这种被低估的作物进行遗传改良。
Genetic Diversity in Egyptian Snake Melon Accessions as Revealed by Inter Simple Sequence Repeat (ISSR) Markers
Snake melon (Cucumis melo var. flexuosus) is native to the region from Egypt to Iran; therefore, local genotypes in Egypt may contain genes responsible for tolerance to biotic and abiotic stresses. Inter simple sequence repeat (ISSR) markers were used to examine genetic relations among 12 Egyptian snake melon accessions collected from different regions. Of the 10 markers tested, 6 produced 68 amplification products, of which, 23 were polymorphic (33.7% polymorphism). The average polymorphism information content (PIC) value was 0.68 over the 6 primers. Pairwise Jaccord's similarity coefficient ranged from 0.88 to 0.98, indicating a narrow diversity. Cluster analysis distinguished two clusters, one from Cairo, and the second represents the rest of accessions from different regions. It was shown that snake melon genotypes that were genetically close were not necessarily close in geographical distance. On a level of non-coding region using 6 ISSR primers, some differences were found among the Egyptian snake melon accessions, confirming the usefulness of the technique in detecting genetic diversity which may help in future genetic improvement programs in this underestimated crop.