附加营养素和胶凝剂对选定籼稻品种体外反应的影响

S. K. Repalli, Chaitanya Kumar Geda, N. Pradhan, G. Rao
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引用次数: 4

摘要

籼稻品种对培养有抗性,因此应在培养基中补充额外的营养物质,以提供能量和渗透潜力,以获得最佳的体外反应。植物生长调节剂的组合对所选基因型的愈伤组织诱导和再生潜力有深远的影响。此外,胶凝剂的浓度和选择对籼稻品种的再生也有影响。探讨了l -脯氨酸和酪蛋白水解物对籼稻品种组织培养反应的影响,阐明了胶凝剂的最佳选择及其体外反应。
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Influence of Additional Nutrients and Gelling Agents on in Vitro Response of Selected Indica Rice Varieties
Indica rice varieties are recalcitrant to culture and hence the culture media should be supplemented with additional nutrients to provide energy and osmotic potential for best in vitro response. Combinations of plant growth regulators have profound influence on callus induction and regeneration potential of the selected genotypes. In addition, concentration and choice of gelling agents also have their effect on regeneration of indica rice varieties. Impact of L-Proline, and Casein Hydrolysate on tissue culture response of selected indica rice varieties is discussed and the best choice of gelling agent and their in vitro response is elucidated.
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