反相高效液相色谱法测定盐酸苯哲索片剂和尿液中的含量

Analytical chemistry, an Indian journal Pub Date : 2010-12-01 DOI:10.21608/ABSB.2010.7380

R. Farag, Ashraf M. Ahmed

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引用次数: 0

摘要

建立了一种快速、灵敏的反相高效液相色谱法测定盐酸苯甲索片剂和尿液中的含量。流动相为乙腈和水，(50% v/v)用磷酸调节pH至5。在C18反相柱(250 mmiÂ´4 mm .d.)上进行分离。流速0.6 ml/min，紫外检测波长254nm。盐酸苯甲索的保留时间为7.4 min。校准曲线在40iÂ μ -g /mL范围内呈线性。盐酸苯哲索的平均回收率为101.16。白天和白天之间的测定都很精确。该方法在治疗浓度或毒性浓度下均具有良好的灵敏度、回收率、准确性和重现性。普通赋形剂不干扰。

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RP HPLC determination of benzhexol hydrochloride in tablet formulations and urine

Anew, rapid and sensitive reverse phase HPLC method was developed and validated for the determination of benzhexol hydrochloride in tablet formulations and urine. The mobile phase used acetonitrile and water, (50 %v/v) adjust pH to 5 using phosphoric acid. The separation was achieved on C18 reversed-phase column (250 mmiÂ´4 mmi.d.). The flowrate was 0.6 ml/min and UV detection at 254nm. The retention time for benzhexol hydrochloride was 7.4 min. The calibration curve was linear up to 40iÂg/mL. The mean recovery for benzhexol hydrochloride is 101.16. The assaywas precisewithin day and between days. The method provided excellent sensitivity, recovery, accuracy and reproducibility in therapeutic or toxic concentrations. Common excipients do not interfere.

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Analytical chemistry, an Indian journal

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