葡萄抗浆原病基因型中激素信号与防御基因表达的相互作用

M. D. Rossarolla, T. C. Tomazetti, L. Welter, H. P. Santos, M. Stefanini, O. Trapp, M. P. Guerra, R. Nodari
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引用次数: 1

摘要

本研究旨在探讨葡萄品种侵染葡萄浆原菌过程中植物防御的相关途径。植株材料包括‘霞多丽’(不含Rpv)、‘摄政’(Rpv3-1)、‘布朗纳’(Rpv3-3+Rpv10)、‘Calardis Blanc’(Rpv3-1+Rpv3-2)和选材GF15 (Rpv1+Rpv3-1)。对‘Regent’、GF15、‘Bronner’和‘Chardonnay’进行了基因表达分析。测定茉莉酸(JA)、水杨酸(SA)、脱落酸(ABA)、吲哚-3-乙酸(IAA)和反式玉米蛋白核糖(tZR)的激素含量。样品采集自离体接种葡萄浆原菌孢子囊的植株,分别于接种后0、1、3、5、7 d采集,用于基因表达;0、3、5和7 DPI用于激素定量。结果表明,与病原菌识别相关的基因表达和激素通路的基因型与接种后时间存在交互作用。茉莉酸和水杨酸都参与了抗性反应。二苯乙烯在不同时间对病原菌的作用也得到了证实。在所有评估的基因型中都观察到与细胞防御相关的基因表达的变化;然而,含有r -基因座的基因型比没有r -基因座的品种反应更快,激活细胞死亡机制,导致过敏症状。
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The interplay between hormone signaling and defense gene expression in grapevine genotypes carrying genetic resistance against Plasmopara viticola
The present study aimed to investigate plant defense related pathways during Plasmopara viticola infection in Vitis vinifera varieties. Plant material consisted of 'Chardonnay' (no Rpv), 'Regent' (Rpv3-1), 'Bronner' (Rpv3-3+Rpv10), 'Calardis Blanc' (Rpv3-1+Rpv3-2), and the breeding selection GF15 (Rpv1+Rpv3-1). Gene expression analysis was carried out for the varieties 'Regent', GF15, 'Bronner', and 'Chardonnay'. Hormonal quantification was performed for jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA), indole-3-acetic acid (IAA), and trans-zeatin-ribose (tZR). The samples were collected from plants cultivated in vitro inoculated with Plasmopara viticola sporangia, and collected at 0, 1-, 3-, 5-, and 7-days post inoculation (DPI) for gene expression; and 0, 3, 5, and 7 DPI for hormonal quantification. The results showed an interaction between genotype and time post inoculation in gene expression and hormonal pathways linked with pathogen recognition. Both jasmonate and salicylic acids were involved in the resistance response. The role of stilbenes acting against the pathogen at different times was also confirmed. Changes in the expression of genes linked to cell defense were observed in all evaluated genotypes; however, genotypes with R-loci responded more quickly than the variety without R-loci, activating mechanisms of cell death, resulting in symptoms of hypersensitivity.
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