不同工业大麻(cannabis sativa (Linnaeus 1753)(大麻科))基因型提取物对Diuraphis noxia Kurdjumov, 1913, Myzus persicae Sulzer, 1776和fabae Scopoli, 1763的影响(半翅目:蚜虫科)

Ş. Şimşek, A. Kayahan, Gamze Pekbey, G. Yilmaz, I. Karaca
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摘要

本文研究了三种不同基因型(Narlısaray、Kavacık、Maltepe)大麻甲醇提取物对库尔朱莫氏盲蝽(Diuraphis noxia Kurdjumov)、桃蚜(Myzus persicae)和fabae (Scopoli)(半翅目:蚜虫科)的影响。在研究的第一阶段,采用喷雾法在蚜虫的第2和第3若虫期施用10%浓度的大麻提取物。在24 - 48和72小时后,记录活鼠和死鼠,并确定死亡率。在第二阶段,使用效果最高的基因型进行剂量-死亡试验,并指定不同剂量(2.5%、5%、7.5%和10%)下的LD50和LD90值。在72 h后的普查中,Narlısaray基因型的死亡率最高,为54.04%。Kavacık基因型对桃蚜的毒力为23.13%,对蚕豆蚜的毒力最高,为91.76%。根据Kavacık基因型对fabae的剂量测定研究,计算出LD50和LD90值分别为0.33和0.110 (mg/个体)。根据研究结果,已观察到工业大麻植物的不同基因型提取物对蚜虫物种有效,并认为它们可用于控制这些害虫。
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Effects of different industrial cannabis (Cannabis sativa (Linnaeus 1753) (Cannabaceae)) genotype extracts on Diuraphis noxia Kurdjumov, 1913 Myzus persicae Sulzer, 1776 and Aphis fabae Scopoli, 1763 (Hemiptera: Aphididae)
In this study, the effect of methanol extracts of three different genotypes (Narlısaray, Kavacık, Maltepe) of Cannabis sativa L. on Diuraphis noxia Kurdjumov, Myzus persicae (Sulzer) and Aphis fabae (Scopoli) (Hemiptera: Aphididae) were investigated. In the first stage of the study, 10% concentrations of each cannabis extract were applied on the 2nd and 3rd nymphal stages of aphid species by spraying method. After the end of 24 - 48 and 72 hours of the applications, the alive and dead individuals were recorded and mortality rates were determined. In the second stage, the genotype with the highest effect was used in dose-death trials and LD50 and LD90 values at different doses (2.5%, 5%, 7.5% and 10%) were specified. In the census after 72 hours, Narlısaray genotype showed the highest mortality rate with 54.04% on D. noxia. While the effect of Kavacık genotype on M. persicae was found as 23.13%, the highest toxicity record of the same genotype was determined on A. fabae (as 91.76%). According to the dose measurement studies of Kavacık genotype on A. fabae, LD50 and LD90 values were calculated to be 0.33 and 0.110 (mg/individual), respectively. At the results of study, it has been observed that extracts of different genotypes of the industrial cannabis plant are found effective on aphid species and it is thought that they can be used in controlling of these pests.
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