dATP诱导细胞游离凋亡的光电分析方法

Y. Ci, Chun-yang Zhang, Jun Feng, Ai-dong Lang, Chun‐hui Huang, Zhengfan Jiang
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引用次数: 2

摘要

利用ITO(一种氧化铟锡包覆硼硅酸盐的透明电极)技术,建立了一种新的分析细胞游离凋亡的光电方法。C5710小鼠肝核对白光(200-800nm)有负电电流脉冲响应。在dATP(脱氧腺苷-5-三磷酸)诱导的肝核凋亡过程中,光电流呈动态下降,这与荧光显微镜和琼脂糖凝胶电泳结果一致。这种光电分析方法为快速、连续地评价细胞凋亡提供了一种快速、灵敏的方法。
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A photoelectric method for analyzing cell-free apoptosis induced by dATP
A new photoelectric method for analyzing cell-free apoptosis had been developed with ITO (a transparent electrode of indium–tin oxide coated borosilicate) technology. The C5710 mice liver nuclei responded with a negative photoelectric current pulse to white light (200–800nm). During the apoptosis of liver nuclei induced by dATP (deoxyadenosine-5-triphosphate), the photoelectric current showed a dynamic decrease, which was in accordance with the results of fluorescence microscopy and agarose gel electrophoresis. This photoelectric analytical method might provide a rapid and sensitive way to evaluate apoptosis quickly and in a continuous fashion.
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