聚乙二醇化纤维蛋白原的生物相容性及其对大鼠全层皮肤缺损愈合作用的初步研究

Venzin Cm, Jacot, A. Berdichevsky, Karol Aa, D. Seliktar, B. vonRechenberg, Nuss Kmr
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引用次数: 4

摘要

一种合成聚合物聚乙二醇(PEG),与纤维蛋白原偶联,作为三维可生物降解的皮肤伤口敷料基质。这种聚乙二醇纤维蛋白原(PEG-fib)通过注射进入伤口并通过光激活原位聚合后,在皮肤伤口时间过程研究中测试了其生物相容性和生物降解。材料与方法:在7天(n=6)和14天(n=6)对大鼠急性、直径8 mm、全层皮肤病变植入材料的炎症反应进行组织学评价。每个时间点有6个伤口未经治疗作为对照组。结果:14 d后,两组创面收缩率达78%,上皮化率达22%。免疫细胞如异物巨细胞、巨噬细胞、浆细胞和淋巴细胞在两个时间点均可见PEGfib处理的伤口,但数量较少,与对照组相似。免疫细胞数量在第7天至第14天之间下降。第7天在两个PEG-fib处理的伤口中发现了凝胶的残留物,14天后在任何伤口中都没有发现PEG-fib。两种治疗在两个时间点上的上皮化没有差异。讨论:组织学评价显示PEG-fib具有良好的生物相容性,因此可以排除异物对植入物的反应。免疫细胞的数量符合对植入可吸收生物材料的正常反应。结论:聚乙二醇纤维水凝胶作为皮肤创面敷料具有良好的生物相容性。它为伤口床提供最初的水分,并逐渐被吸收并被结构化的皮肤组织所取代。一个有吸引力的未来前景是在PEG-fib水凝胶中预先填充细胞(如成纤维细胞),或在其上加载生长因子或其他可溶性介质,以进一步促进复杂皮肤伤口的愈合。
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Biocompatibility of pegylated fibrinogen and its effect on healing of full-thickness skin defects: A preliminary study in rats
Introduction: A synthetic polymer polyethylene glycol (PEG), was conjugated to fibrinogen as a threedimensional and biodegradable skin wound dressing matrix. This PEG-fibrinogen (PEG-fib) was tested in vivo in a skin wound time course study for its biocompatibility and biodegradation, after being delivered into the wound by injection and polymerized in situ by photo-activation. Materials and methods: The nature of the inflammatory response to the implanted material in acute, 8 mm diameter, full-thickness skin lesions in rats was histologically evaluated at 7 days (n=6) and 14 days (n=6). Six wounds per time point were left untreated as controls. Results: After 14 days, wounds of both groups were healed by up to 78% contraction and 22% epithelialization. Immune cells such as foreign body giant cells, macrophages, plasma cells and lymphocytes were seen in the PEGfib treated wounds at both time points, however in low numbers and similar to controls. The amount of immune cells dropped between day 7 and 14. Remnants of the gel were found at day 7 in two of the PEG-fib treated wounds, no PEG-fib were found after 14 days in any of the wounds. There was no difference in epithelialization between the two treatments at both time points. Discussion: The histological evaluation showed good biocompatibility of the PEG-fib, such that a foreign body reaction to the implant could be ruled out. The amount of immune cells was in accordance to a normal reaction to an implanted resorbable biomaterial. Conclusion: The PEG-fib hydrogel is fully biocompatible as a skin wound dressing. It provides initial moisture to the wound bed and is gradually resorbed and replaced by structured skin tissue. An attractive future perspective would be to prepopulate the PEG-fib hydrogel with cells (e.g. fibroblasts), or load it with growth factors or other soluble mediators to further promote healing of complicated skin wounds.
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