间充质干细胞条件介质如何影响HeLa细胞的Wnt/ β -连环蛋白信号、Notch-1信号和凋亡?

Hanife Guler Donmez, Handan SEVİM AKAN
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引用次数: 0

摘要

本研究旨在探讨间充质干细胞(MSCs)细胞条件培养基(MSCs- cm)对宫颈癌细胞Wnt/ β -catenin和Notch-1信号通路及凋亡的影响。鉴定的MSCs的条件培养基在使用前新鲜收集并过滤。海拉细胞在标准条件下培养,用msc - cm处理24,48,72小时。未处理的细胞作为对照。在所有潜伏期用MTT法测定细胞活力。每个时间点对β -catenin、Notch-1和cleaved caspase 3进行免疫细胞化学染色。MTT细胞活力、AO/PI、cleaved caspase 3免疫细胞化学染色结果显示,在所有孵育期间,MSCs-CM处理的HeLa细胞与对照组比较,差异均无统计学意义(p < 0.05)。治疗24小时至48小时和72小时后β -连环蛋白免疫反应性上调(p
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How mesenchymal stem cell conditioned media affect the HeLa cells on Wnt/beta-catenin signaling, Notch-1 signaling, and apoptosis?
This study aims to investigate the influence of mesenchymal stem cells (MSCs) cell-conditioned media (MSCs-CM) on the Wnt/beta-catenin and Notch-1 signaling as well as the apoptosis in cervical cancer cells. Conditioned media of characterized MSCs were freshly collected and filtered before use. HeLa cells cultured standard conditions and treated with MSCs-CM 24, 48, 72 hours. Untreated cells serve as a control. Cell viability measured with MTT assay for all incubation periods. Immunocytochemical staining of beta-catenin, Notch-1 and cleaved caspase 3 were performed for each time-point. MTT cell viability, AO/PI, and immunocytochemical staining of cleaved caspase 3 results showed that through all incubation periods, there was no statistically significant difference between the MSCs-CM treated HeLa cells and the controls (p>0.05). Beta-catenin immunoreactivity was upregulated following treatment from 24 hours to 48 and 72 hours (p
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