以花椰菜茎为底物的内生曲霉生产纤维素酶的研究

D. Prabavathy, C. Nachiyar
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引用次数: 7

摘要

农业和工业废物是造成环境污染的主要原因之一。它们转化为有用的产品可能会改善它们造成的问题。本研究以常被废弃的菜花秸秆为底物,生产纤维素酶,纤维素酶是有机废物分解的重要酶。从芦笋中分离得到的内生曲霉能够以花椰菜茎为底物产生具有一定活性的纤维素酶。FPA和CMC试验研究了时间对生物量和纤维素酶活性的影响,结果表明,生长和纤维素酶活性在6 d前逐渐增加,6 d后开始下降。
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Production of cellulase by an Endophytic Aspergillus sp, using Cauliflower stalk as substrate
Agricultural and industrial wastes are among the major causes of environmental pollution. Their conversion to useful products may ameliorate the problems they cause. In this study, Cauliflower stalk commonly discarded as waste material was used as a substrate for the production of cellulase, an important enzyme involved in the breakdown of organic wastes. Endophytic Aspergillus sp. isolated from Adathoda beddomei was found to be capable of producing cellulase with appreciable activity using cauliflower stalk as substrate. The effect of time on biomass production, cellulase activity as studied by FPA and CMC assay indicated that the growth as well as the cellulase activity increased gradually upto 6 days, after which they have started to decrease.
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