从火山土壤中分离的丙烯酰胺降解菌群

.. Rusnam, N. Gusmanizar
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引用次数: 9

摘要

在土壤中,聚丙烯酰胺是丙烯酰胺的主要来源,因为它会慢慢分解成丙烯酰胺。世界范围内对微生物介导的丙烯酰胺分解作为一种生物修复方法的兴趣一直在适度但稳定地上升。在本研究中,从印度尼西亚西苏门答腊岛马拉皮火山土壤中分离出的细菌群能够在丙烯酰胺上茁壮成长。以丙烯酰胺为唯一氮源的丙烯酰胺降解菌在1% (w/v)葡萄糖的存在下生长最好。丙烯酰胺在300至500毫克/升、pH在6.5至8.0之间、温度在30至35°C之间时生长最佳。该菌体也可以在乙酰胺作为唯一氮源的条件下生长。有毒的重金属,如汞、银和铜,减缓了丙烯酰胺联盟的发展。这是首次从火山土壤中分离出丙烯酰胺降解菌群。
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An Acrylamide-degrading Bacterial Consortium Isolated from Volcanic Soi
In soil, polyacrylamide is a key source of acrylamide because it slowly decomposes into acrylamide. There has been a modest but steady rise in worldwide interest in microbe-mediated acrylamide decomposition as a bioremediation method. A bacterial consortium isolated from the volcanic soil of Mount Marapi, West Sumatra, Indonesia, was able to thrive on acrylamide in this study. Acrylamide-degrading bacteria grew best in the presence of 1 %(w/v) glucose with acrylamide as the sole nitrogen source. Optimum growth occurs in between 300 and 500 mg/L of acrylamide, pH between 6.5 and 8.0, and temperatures between 30 and 35 °C. The consortium can also grow on acetamide as the sole nitrogen source. Toxic heavy metals, such as mercury, silver and copper slowed down the growth of this consortium on acrylamide. This is the first report of an acrylamide-degrading consortium isolated from volcanic soils.
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