Effect of the Addition of Egg Yolk to Skim Milk Extender and Storage Time on the Motility and Fertility of Kampung Rooster Spermatozoa

The aim of this study was to evaluate the egg yolk addition and storage time on the motility and fertility of Kampung rooster spermatozoa. A completely randomized design with a 3x2 factorial pattern was used in this study. E factor is the addition of egg yolk into skim milk. (E1 = skim milk + egg yolk 15%, E2 = skim milk + egg yolk 20%, E3 = skim milk + egg yolk 25%) and S factor is storage time (S1 = 60 minutes, S2 = 180 minutes), at 5 ° C. Pooled 10 kampung rooster semen was allocated into six experimental groups. Motility assessment in each treatment group was carried out five times. Fertility assessment was obtained by inseminating ten hens of Isa Brown (n=60) for each treatment for seven days egg collection. There is no interaction effect between storage time and egg yolk addition (P>0.05) on the motility and fertility of kampung rooster sperm. The mean percentage motility and fertility of kampung rooster sperm in extender containing different egg yolk were (72.5±1.73; 67.5±1.73; 65.83±1.81%, P>0.05), and (94.13±9.35; 88.64±8.97 and 91.61±11.26; P>0.05), respectively. The average sperm motility and Fertility in a different storage time were (70.83±3.53% and 67.78±3.47%, P>0.05) and (91.06±5.77% and 91.86±3.26%, P>0.05), respectively. It can be concluded that the addition of 15, 20 and 25 % egg yolk to skim milk stored at 5°C refrigerator temperature for up to three hours can be used to dilute Kampung rooster semen.