大豆皮蛋白的提取、糊化及抗氧化性能研究

Sean X. Liu, Diejun Chen, Mukti Singh, Jingyuan Xu
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引用次数: 4

摘要

大豆皮是豆油和豆粕加工的副产品,约占整个种子的8%。本文研究了大豆皮的持水性和糊化性能,并首次报道了大豆皮中对人体长期健康具有重要意义的酚类物质的含量和抗氧化活性。此外,还研究了从大豆皮中提取蛋白质的最佳pH条件、均质和分离方法。在pH为9的条件下,提取液和回收液中蛋白质含量较高。采用筛分法从壳中提取蛋白质可能是一种工业应用的有效方法。水洗一次纯化后,沉淀蛋白含量由51.52%提高到59.29%;然而,经过两次洗涤后,没有进一步的改善。提取的蛋白质可用于食品应用。磨碎的壳粉(10%蛋白质),干燥的上清(14%蛋白质)和沉积物(7-8%蛋白质)以及有价值的纤维应该是几种食品类别的良好食品成分。本研究探索了将低价值副产品转化为增值功能食品的巨大潜力,以及减少粮食和农业浪费的好处。
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Extraction of Proteins and Pasting and Antioxidant Properties of Soybean Hulls
Soybean hulls are a by-product from soybean processing for oil and meal production which comprise approximately 8% of the whole seed. This study investigated water holding capacities and pasting properties, and first reported the phenolic contents and antioxidant activities from soybean hulls which are important to our long-term health. In addition, the conditions for extracting proteins from soybean hulls including optimum pH, as well as homogenizing and separation methods for extraction, were also studied. Higher protein content in extracts and recoveries was obtained with extraction at pH 9. Using sieve separation may be an effective way to extract proteins from hulls for industrial applications. The precipitated protein content increased from 51.52% to 59.29% after purification by washing with water once; however, after two washes, no further improvement was shown. The extracted proteins can be used for food applications. The ground hull powders (10% protein), dried supernatant (14% protein) and sediments (7-8% proteins) along with valuable fibers should be good food ingredients for several food categories. This research explored the great potential of converting the low value by-products into value-added functional food uses along with the benefit of reducing food and agricultural wastes.
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