肺炎克雷伯菌抗菌次级代谢物的气相色谱-质谱联用研究及其活性天然化合物的筛选

I. Hameed, Sahar Sajjad Saad Zghair, Sarhan Thajeel
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引用次数: 0

摘要

微生物次生代谢产物是具有特殊结构的低分子质量产物。结构多样的代谢物表现出多种生物活性,如抗菌药物。从肺炎克雷伯菌甲醇提取物中鉴定出39种活性物质。GC-MS分析发现,肺炎克雷伯菌中存在三环[4.3.1.1(3.8)]十一胺,3-甲氧基苯甲醛缩氨基酮,甲醛,1-甲基-,肟,(Z)-(+), 1,5,5-三甲基-6-亚甲基-环己烯,4-(2,5-二氢-3-甲氧基苯基)丁胺,帕罗霉素,9-硼比环[3.31]壬烷,9-巯基-,苯乙醇,2-(2-氨基丙氧基)-3-甲基,乙酰胺,N-(6-乙酰氨基苯并噻唑-2-基)-2-(金刚烷),环-6-羧酸,(4) - 2, 5-Dihydro-3-methoxyphenyl丁胺,N - (2 5-Dicyano-3 4-dihydro-2H-pyrrol-2-yl)乙酰胺,3,10-Dioxatricyclo[4.3.1.0(2、4)]dec-7-ene, 3-Cyclohex-3-enyl-propionic酸、花生酸、phenylmethyl酯,3,7-Diazabicyclo[3.3.1]壬烷,9日9-dimethyl,硫代氨基甲酸,S-methyl - N - (2-methyl-3-oxobutyl), dl-Homocysteine, 2 - (2-Furyl)吡啶,1,7-Dioxa-10-thia-4, 13-diazacyclopentadeca-5, 9日12-trione, 5, 7-Dodecadiyn-1, 12-diol, 1 -(β-d-Arabinofuranosyl) 4-o-difluoromethyluracil,尿酸,Pyrrolo [1.2] pyrazine-1、4-dione hexahydro, 12-Methyl-oxa-cyclododecan-2-one,邻苯二甲酸、丁十一烷基酯,9日,12日,15-Octadecatrienoic酸,2,二(acetyloxy)丙酯,1,2,4-Trioxolane-2-octanoic酸5-octyl,甲酯,12-Dimethylamino-10-oxododecanoic酸,Octahydrochromen-2-one,天门冬氨酸,N-glycyl - 2 h-oxecin-2-one, 3, 4, 7, 8, 91年,10-hexahydro-4-hydroxy-10-meth, Thiazolo(4、5 d) pyrimidine-5 7 (4 h、6 h)土卫四,2-amino-4 (2-ph Dec-9-en-6-oxo-1-ylamide,3,6,12-三甲基-1,4,7,10,13,16-六氮杂环十二烷,2 -lodohiistidine, 2,5-哌嗪二酮,3,6-二(2-甲基丙基)-,9-十八烯酰胺,(Z)-, 3',8,8'-三甲氧基-3-哌啶基-2,2'-联萘-1,1',4,4'-四元。临床病原菌抗菌活性分别为肺炎链球菌、大肠杆菌、金黄色葡萄球菌、奇异变形杆菌、表皮葡萄球菌,细菌产物(肺炎克雷伯菌产生的代谢物)抗菌活性分别为4.09±0.013、2.99±0.300、4.37±0.200、3.22±0.210、4.00±0.203,细菌产物链霉素抗菌活性分别为1.08±0.200、0.97±0.116、2.08±0.233、3.04±0.261、0.98±0.166,抗菌活性分别为1.02±0.180;卡那霉素抗菌药物分别为1.00±0.190、2.08±0.236、1.00±0.100、1.82±0.200。肺炎克雷伯菌产生许多重要的次生代谢产物,具有较高的生物活性。基于利用生物活性化合物在制药中生产治疗许多疾病的药物的意义,对肺炎克雷伯菌产生的化合物进行纯化可能是有用的。
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Characterization of antimicrobial secondary metabolites produced by Klebsiella pneumoniae and screening of its bioactive natural compounds using gas chromatography-mass spectrometry (GC-MS)
Microbial secondary metabolites are low molecular mass products with unusual structures. The structurally diverse metabolites show a variety of biological activities like antimicrobial agents. Thirty nine bioactive compounds were identified in the methanolic extract of Klebsiella pneumoniae. GC-MS analysis of Klebsiella pneumoniae revealed the existence of the Tricyclo[4.3.1.1(3.8)]undecan-1-amine, 3-Methoxybenzaldehyde semicarbazone, carboxaldehyde , 1-methyl-,oxime ,(Z)-(+), 1,5,5-Trimethyl-6-methylene-cyclohexene, 4-(2,5-Dihydro-3-methoxyphenyl)butylamine, Paromomycin , 9-Borabicyclo[3.31]nonane , 9-mercapto-, Benzenemethanol , 2-(2-aminopropoxy)-3-methyl, Acetamide , N-(6-acetylaminobenzothiazol-2-yl)-2-(adamantan, rin-6-carboxylic acid , 4-(2,5-Dihydro-3-methoxyphenyl)butylamine, N-(2,5-Dicyano-3,4-dihydro-2H-pyrrol-2-yl)-acetamide, 3,10-Dioxatricyclo [4.3.1.0(2,4)]dec-7-ene, 3-Cyclohex-3-enyl-propionic acid, Eicosanoic acid ,phenylmethyl ester, 3,7-Diazabicyclo[3.3.1]nonane , 9,9-dimethyl-, Dithiocarbamate , S-methyl-,N-(2-methyl-3-oxobutyl)-, dl-Homocysteine, 2-(2-Furyl)pyridine, 1,7-Dioxa-10-thia-4,13-diazacyclopentadeca-5,9,12-trione, 5,7-Dodecadiyn-1,12-diol, 1-(β-d-Arabinofuranosyl)-4-O-difluoromethyluracil, Uric acid, Pyrrolo[1.2-a]pyrazine-1,4-dione , hexahydro-,12-Methyl-oxa-cyclododecan-2-one, Phthalic acid , butyl undecyl ester, 9,12,15-Octadecatrienoic acid , 2,3-bis(acetyloxy)propyl ester, 1,2,4-Trioxolane-2-octanoic acid 5-octyl-, methyl ester, 12-Dimethylamino-10-oxododecanoic acid , Octahydrochromen-2-one, L-Aspartic acid , N-glycyl-,2H-Oxecin-2-one , 3,4,7,8,91,10-hexahydro-4-hydroxy-10-meth , Thiazolo[4,5-d]pyrimidine-5,7(4H,6H)-dione , 2-amino-4-(2-ph, Dec-9-en-6-oxo-1-ylamide, 3,6,12-Trimethyl-1,4,7,10,13,16-hexaaza-cyclooctadecane, 2-lodohiistidine, 2,5-Piperazinedione ,3,6-bis(2-methylpropyl)-, 9-Octadecenamide , (Z)-, 3',8,8'-Trimethoxy-3-piperidyl-2,2'-binaphthalene-1,1',4,4'-tetra. Clinical pathogens selected for antibacterial activity namely, Streptococcus pneumonia, Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Staphylococcus epidermidis, It were 4.09±0.013, 2.99±0.300, 4.37±0.200, 3.22±0.210, and 4.00±0.203 respectively for Bacterial products (Metabolites Produced by Klebsiella pneumoniae), while recorded 1.08±0.200, 0.97±0.116, 2.08±0.233, 3.04±0.261, 0.98±0.166 respectively for Bacterial products Streptomycin antibiotics, and recorded 1.02±0.180, 1.00±0.190, 2.08±0.236, 1.00±0.100, and 1.82±0.200 respectively for Kanamycin antibiotics. Klebsiella pneumoniae produce many important secondary metabolites with high biological activities. Based on the significance of employing bioactive compounds in pharmacy to produce drugs for the treatment of many diseases, the purification of compounds produced by Klebsiella pneumoniae can be useful.
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