脱氢酶AmDH1提高转基因玉米植株的抗旱性

Huynh Thi Thu Hue, Le Quynh Lien, Nguyen Chi Mai, Tran My Linh, Bui Manh Minh
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引用次数: 0

摘要

脱氢蛋白是一种对植物细胞在渗透胁迫下具有保护作用的大蛋白群。从红树林植物中分离的过表达脱氢编码基因提高了转基因植物的抗旱性。因此,本研究从越南宣翠国家公园天然生长的红树林植物Mam bien (Avicennia marina)中分离出一个编码Dehydrin1 (DH1)蛋白的编码序列,全长573bp。编码序列命名为AmDH1,将其克隆到植物转化载体pCAM 35S-AmDH1中。将该转基因载体转化为A. tummefiaciens细胞,并将其转入K7玉米株系,获得转基因玉米幼苗。AmDH1转基因玉米T2幼苗的抗旱性比原玉米K7株系有所提高。
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Dehydrin AmDH1 improves drought tolerance in transgenic maize plants
Dehydrin is a large protein group to have protective functions to plant cells under osmotic stress. Overexpression of dehydrin encoded genes isolated from mangrove plants had improved drought tolerance of transgenic plants. Therefore, in this research, a coding sequence with the length of 573bp that encodes Dehydrin1 (DH1) protein was isolated from a mangrove plant namely Mam bien (Avicennia marina) which naturally grows in Xuan Thuy national park, Vietnam. The coding sequence was denoted as AmDH1 and cloned into the plant transformation vector pCAM 35S-AmDH1. The transgenic vector was transformed into A. tumefiaciens cells and the vector was transferred into the K7 maize line to generate transgenic maize seedlings. The AmDH1 transgenic maize T2 seedlings have shown an improved drought tolerance than the original K7 maize line.
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